Construct: ORF TRCN0000470031
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001048.1_s317c1
- Derived from:
- ccsbBroadEn_14044
- DNA Barcode:
- TCACGCTCTAAATTTCTGACACAG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- PCGF3 (10336)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470031
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10336 | PCGF3 | polycomb group ring finger 3 | NM_001317836.2 | 99.8% | 78.1% | 570_571insA |
| 2 | human | 10336 | PCGF3 | polycomb group ring finger 3 | NM_006315.6 | 99.8% | 78.1% | 570_571insA |
| 3 | mouse | 69587 | Pcgf3 | polycomb group ring finger 3 | NM_172716.4 | 88% | 75.7% | (many diffs) |
| 4 | mouse | 69587 | Pcgf3 | polycomb group ring finger 3 | XM_006535208.2 | 80% | 70.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 663
- ORF length:
- 594
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gttgaccagg aagatcaagc tgtgggacat caacgcccac atcacctgcc 121 gcctgtgcag cgggtacctc atcgacgcca ccacggtgac cgagtgtctg cacaccttct 181 gcaggagctg cctggtgaag tacctggagg agaacaacac ctgccccacc tgcaggattg 241 tgatccacca gagccacccc ctgcagtaca tcggtcatga cagaaccatg caagatattg 301 tttacaaatt ggtaccaggc ctccaagaag cggaaatgag aaagcagagg gagttctatc 361 acaaattGGG CATGGAGGTG CCGGGAGACA TCAAGGGGGA GACCTGCTCT GCAAAACAGC 421 ACTTAGATTC CCATCGGAAT GGTGAAACCA AAGCAGACGA CAGTTCAAAC AAAGAGGCCG 481 CGGAGGAGAA GCCGGAGGAG GACAACGACT ACCACCGCAG CGACGAGCAG GTGAGCATCT 541 GCCTGGAGTG TAACAGCAGC AAACTGCGCG GGCTGAAGCG GAAGTGGATC CGCTGCTCAG 601 CCCAGGCGAC CGTCTTGCAT CTGAAGAAGT TCATCGCCAA AAAAACTCAA CCTTTCATCC 661 TTTAACGAGC TGGACATTTT ATGCAACGAG GAGATCCTGG GCAAGGACCA CACACTCAAG 721 TTCGTGGTTG TCACTAGGTG GAGATTCAAG AAGGCGCCGC TCCTGCTGCA CTACAGACCC 781 AAGATGGACT TGCTGTTGCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC 841 CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGCCCGTA ACTTGAAAGT 901 ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG ATCACGCTCT AAATTTCTGA 961 CACAGACGCG TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt