Construct: ORF TRCN0000470033
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002358.1_s317c1
- Derived from:
- ccsbBroadEn_00139
- DNA Barcode:
- TTCTCGCCAATTACAGACCAGTTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ATP6V1G2 (534)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470033
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
---|---|---|---|---|---|---|---|---|
1 | human | 534 | ATP6V1G2 | ATPase H+ transporting V1 s... | NM_130463.4 | 100% | 100% | |
2 | human | 534 | ATP6V1G2 | ATPase H+ transporting V1 s... | NM_001204078.2 | 66.1% | 66.1% | 100_101ins120 |
3 | human | 534 | ATP6V1G2 | ATPase H+ transporting V1 s... | NM_138282.3 | 65.2% | 65.2% | 0_1ins123 |
4 | mouse | 66237 | Atp6v1g2 | ATPase, H+ transporting, ly... | NM_023179.3 | 89.8% | 94.9% | (many diffs) |
5 | mouse | 66237 | Atp6v1g2 | ATPase, H+ transporting, ly... | NM_001347351.1 | 58.1% | 61% | (many diffs) |
6 | mouse | 66237 | Atp6v1g2 | ATPase, H+ transporting, ly... | XM_006524795.2 | 58.1% | 61% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 93
- ORF end:
- 447
- ORF length:
- 354
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA CCATGGCCAG TCAGTCCCAA GGTATCCAGC 121 AGCTTCTGCA AGCTGAGAAG CGGGCAGCTG AGAAGGTGGC AGATGCCAGA AAGAGGAAGG 181 CCCGGCGACT GAAGCAGGCA AAGGAGGAGG CACAGATGGA GGTGGAGCAA TACCGCAGAG 241 AGCGAGAGCA CGAATTCCAG AGCAAGCAGC AGGCGGCCAT GGGCTCCCAG GGGAACCTGT 301 CTGCTGAGGT GGAGCAGGCT ACAAGGCGCC AGGTGCAGGG CATGCAGAGC TCCCAGCAGA 361 GAAACCGAGA GCGTGTCCTG GCCCAGCTTC TTGGCATGGT CTGCGACGTC AGGCCCCAGG 421 TCCACCCCAA CTACCGGATT TCTGCCTTGC CAACTTTCTT GTACAAAGTG GTTGATATCG 481 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 541 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GATTCTCGCC 601 AATTACAGAC CAGTTTACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 661 aagatt