Construct: ORF TRCN0000470033

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF002358.1_s317c1
Derived from:
ccsbBroadEn_00139
DNA Barcode:
TTCTCGCCAATTACAGACCAGTTT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ATP6V1G2 (534)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000470033
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 534 ATP6V1G2 ATPase H+ transporting V1 s... NM_130463.4 100% 100%
2 human 534 ATP6V1G2 ATPase H+ transporting V1 s... NM_001204078.2 66.1% 66.1% 100_101ins120
3 human 534 ATP6V1G2 ATPase H+ transporting V1 s... NM_138282.3 65.2% 65.2% 0_1ins123
4 mouse 66237 Atp6v1g2 ATPase, H+ transporting, ly... NM_023179.3 89.8% 94.9% (many diffs)
5 mouse 66237 Atp6v1g2 ATPase, H+ transporting, ly... NM_001347351.1 58.1% 61% (many diffs)
6 mouse 66237 Atp6v1g2 ATPase, H+ transporting, ly... XM_006524795.2 58.1% 61% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
93
ORF end:
447
ORF length:
354
Sequence:
1TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT
61GATCAACAAG TTTGTACAAA AAAGTTGGCA CCATGGCCAG TCAGTCCCAA GGTATCCAGC
121AGCTTCTGCA AGCTGAGAAG CGGGCAGCTG AGAAGGTGGC AGATGCCAGA AAGAGGAAGG
181CCCGGCGACT GAAGCAGGCA AAGGAGGAGG CACAGATGGA GGTGGAGCAA TACCGCAGAG
241AGCGAGAGCA CGAATTCCAG AGCAAGCAGC AGGCGGCCAT GGGCTCCCAG GGGAACCTGT
301CTGCTGAGGT GGAGCAGGCT ACAAGGCGCC AGGTGCAGGG CATGCAGAGC TCCCAGCAGA
361GAAACCGAGA GCGTGTCCTG GCCCAGCTTC TTGGCATGGT CTGCGACGTC AGGCCCCAGG
421TCCACCCCAA CTACCGGATT TCTGCCTTGC CAACTTTCTT GTACAAAGTG GTTGATATCG
481GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT
541AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GATTCTCGCC
601AATTACAGAC CAGTTTACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga
661aagatt

Download FASTA (ORF) (Full)