Construct: ORF TRCN0000470050
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016297.1_s317c1
- Derived from:
- ccsbBroadEn_02567
- DNA Barcode:
- GCGGTGGTACATGGCCCCTTATCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- C11orf58 (10944)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470050
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10944 | C11orf58 | chromosome 11 open reading ... | NM_014267.6 | 100% | 100% | |
| 2 | human | 10944 | C11orf58 | chromosome 11 open reading ... | XM_017017143.2 | 75.9% | 75.9% | 0_1ins132 |
| 3 | mouse | 56372 | 1110004F10Rik | RIKEN cDNA 1110004F10 gene | NM_019772.2 | 86.7% | 95% | (many diffs) |
| 4 | mouse | 56372 | 1110004F10Rik | RIKEN cDNA 1110004F10 gene | XM_011241867.1 | 76.8% | 83.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 615
- ORF length:
- 549
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag tgctgccaga gagtctcacc cgcatggggt gaagcgttca gcctccccag 121 acgacgatct gggatctagc aattgggagg cagcagactt gGGTAATGAA GAGAGAAAAC 181 AAAAGTTCTT GAGACTTATG GGTGCAGGAA AGAAAGAACA TACTGGTCGT CTTGTTATAG 241 GAGATCACAA ATCAACATCT CACTTCCGAA CCGGGGAAGA AGACAAGAAA ATTAATGAAG 301 AACTGGAGTC TCAATATCAG CAAAGTATGG ACAGTAAATT ATCAGGAAGA TATCGGCGAC 361 ATTGTGGACT TGGCTTCAGT GAGGTAGAAG ACCATGATGG AGAAGGTGAT GTGGCTGGAG 421 ATGATGATGA TGACGATGAT GATTCACCTG ATCCTGAAAG TCCAGATGAT TCTGAAAGCG 481 ATTCAGAGTC AGAGAAAGAA GAATCTGCTG AAGAACTCCA AGCTGCTGAG CACCCTGATG 541 AAGTGGAGGA TCCCAAAAAC AAAAAAGATG CAAAAAGCAA TTATAAAATG ATGTTTGTTA 601 AATCCAGTGG TTCATACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 661 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 721 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA GCGGTGGTAC ATGGCCCCTT 781 ATCGACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt