Construct: ORF TRCN0000470064
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006301.1_s317c1
- Derived from:
- ccsbBroadEn_05082
- DNA Barcode:
- GTCAAGTCTTCAATCTTACTTGGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BMT2 (154743)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470064
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 154743 | BMT2 | base methyltransferase of 2... | NM_152556.3 | 100% | 100% | |
| 2 | mouse | 101148 | Bmt2 | base methyltransferase of 2... | NM_175312.4 | 88.8% | 94% | (many diffs) |
| 3 | mouse | 101148 | Bmt2 | base methyltransferase of 2... | XM_011241037.2 | 83% | 84.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1284
- ORF length:
- 1215
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggagccaggg gccggcggcc gaaatactgc tcgtgcgcag agggccgggt 121 ccccgaacac tccgccgccc cgggagcagg agcggaaact ggagcaggag aagctctccg 181 gtgtggtgaa gagcgtccac cggcggctcc gcaagaagta ccgagaagtg ggagattttg 241 ataagatctg gcgagaacac tgtgaggatg aagaaactct ttgtgaatat gccgttgcaa 301 tgaaaaattt ggcagataat cattgggcaa aaacttgtga gggcgaaggt cgtattgaat 361 ggtgttgtag tgtatgcaga gaatatttcc aaaatggtgg gaagagaaaa gcacttgaga 421 aagatgaaaa aagagctgta cttgccacta agaccactcc agccttaaat atgcatgagt 481 cttctcaact tgaaggtcat ttaaccaacc tcagctttac aaaccctgaa tttataactg 541 agttgctaca agcctcagga aaaatcagat tacttgatgt tggcagctgc tttaacccat 601 ttctgaagtt tgaagaattt ctaactgttg gcatagatat tgtacctgct gtagagagtg 661 tctataaatg tgatttcctg aacttacagc ttcagcaacc actccagctt gcacaggatg 721 ctatagatgc ttttttgaag cagctgaaaa accctattga ttctcttcct ggagagcttt 781 tccatgtggt tgttttctct ctccttcttt cttattttcc atctccttac cagcgatgga 841 tttgctgcaa gaaagcccat gaactgttag tgttaaatgg tttattacta atcaTCACAC 901 CTGATTCCTC CCATCAGAAC CGTCATGCTA TGATGATGAA AAGCTGGAAG ATTGCTATAG 961 AGTCCCTGGG CTTTAAACGC TTCAAGTACT CAAAATTTTC ACATATGCAT CTGATGGCAT 1021 TTAGGAAAAT CTCTCTAAAA ACCACAAGTG ACTTGGTTAG TAGGAACTAC CCAGGAATGT 1081 TATATATTCC TCAAGATTTC AACAGTATAG AAGATGAGGA ATATTCTAAC CCTTCCTGCT 1141 ATGTTCGATC AGATATAGAA GATGAACAAC TAGCATATGG TTTCACAGAA CTCCCTGATG 1201 CGCCATATGA CTCAGATTCT GGAGAAAGTC AAGCCAGCTC TATTCCTTTC TATGAGCTAG 1261 AAGACCCCAT ATTACTTTTA AGTTTGCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1321 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1381 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAG TCAAGTCTTC 1441 AATCTTACTT GGCACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1501 att