Construct: ORF TRCN0000470069
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016310.1_s317c1
- Derived from:
- ccsbBroadEn_11631
- DNA Barcode:
- AAAATGCGTTGGCGATTGACGTTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CEP162 (22832)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470069
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 22832 | CEP162 | centrosomal protein 162 | XM_011535594.2 | 69.4% | 68.9% | 1_228del;1797_1798insGTAG;1818_2283del |
| 2 | human | 22832 | CEP162 | centrosomal protein 162 | XR_002956270.1 | 50.9% | 1_597del;2166_2167insGTAG;2187_3112del | |
| 3 | human | 22832 | CEP162 | centrosomal protein 162 | NM_001286206.2 | 39.8% | 39.5% | 1569_1570insGTAG;1590_3981del |
| 4 | human | 22832 | CEP162 | centrosomal protein 162 | XM_006715380.3 | 39.8% | 39.5% | 1569_1570insGTAG;1590_3981del |
| 5 | human | 22832 | CEP162 | centrosomal protein 162 | XM_017010484.2 | 39.8% | 39.5% | 1569_1570insGTAG;1590_3981del |
| 6 | human | 22832 | CEP162 | centrosomal protein 162 | XM_005248674.4 | 38.7% | 38.4% | 1_114del;1683_1684insGTAG;1704_4095del |
| 7 | human | 22832 | CEP162 | centrosomal protein 162 | XM_017010483.2 | 38.7% | 38.4% | 1_114del;1683_1684insGTAG;1704_4095del |
| 8 | human | 22832 | CEP162 | centrosomal protein 162 | NM_014895.4 | 37.7% | 37.4% | 1_228del;1797_1798insGTAG;1818_4209del |
| 9 | human | 22832 | CEP162 | centrosomal protein 162 | XR_001743263.2 | 34.1% | 1_597del;2166_2167insGTAG;2187_4644del | |
| 10 | human | 22832 | CEP162 | centrosomal protein 162 | XR_001743264.2 | 34% | 1_597del;2166_2167insGTAG;2187_4657del | |
| 11 | human | 22832 | CEP162 | centrosomal protein 162 | XR_001743262.2 | 33.3% | 1_597del;2166_2167insGTAG;2187_4763del | |
| 12 | human | 22832 | CEP162 | centrosomal protein 162 | XR_942351.3 | 31.7% | 1_597del;2166_2167insGTAG;2187_5000del | |
| 13 | human | 22832 | CEP162 | centrosomal protein 162 | XM_011535592.3 | 16.3% | 15.9% | 0_1ins939;630_631insGTAG;651_3042del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1659
- ORF length:
- 1593
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga aatagaagag gagtctgctg aaaagattca atttcttaag agcagtggaa 121 cctctctctt aagtactgat agcttagaaa caaatgaact agtagtttct gagctcaacc 181 atagtagtct cggagtggga ttggacacat tagaagaaca agaggagaaa gaacaatttt 241 ttgccaggct tgagaaaggc ttgacatctt ccattgatta ttcgagatta aataaggaat 301 tggattctaa tgactctaca cattttaaag ctttacatag taatcaagcc aacgcagaac 361 taactgatga cgaacatgag aatgaatcga aacatgaaga actggcagaa aattacagtg 421 atgattttga agatgagtat gttggtgcac cgttgactac taaagatgaa gagatgcctt 481 ccaaagagaa ttccaaatca gaaaaaataa gtgtgcccaa acaggaagaa gaaaaaactg 541 gcatgcttgc taatgttgtg ctgcttgatt cattagactc tgttgcagag gtcaatcttg 601 atgaacaaga taaaataaca cctaagccaa ggtgcctacc agaaatgact gagaatgaaa 661 tgacaggaac aggtgtttct tatggacaaa gcagtagtga cgttgaagcc ctacatcaag 721 cttattgtca tatagcccat tcattgggag atgaagacaa acaaaaaatt gagagtaaca 781 cagtggaaga tatcaagagc tcagtgaaag gtcatcctca agaaaatgaa gagaattcaa 841 aaaacatctc tactatggaa tctgatctgc ccacagtaga ggagctgatg aaacctatca 901 gaatagattc ctttgggatc agtggttttg atttacaacc tgtcagctct gagaaagtgg 961 ccgaaagaaa agaaactgaa ttttttagct ctttacccct gaagatgaac ccaaatattt 1021 tgtctcaaga ctcacaacat gtgaaccttt tttttgacaa aaatgatgag aatgtgattt 1081 tacaaaagac cacaaatgag agtatggaaa acagctgtcc acaagtaact gaagtaactg 1141 ccacagaaga acatgttgat aaaatgtacc ttaatatttt gaggaaaaaa ataactgtta 1201 attcttcatc attatctcag gatgacaaaa ttaataaaac ttacagatct caacttagtt 1261 ctgaagaaga agggGCTGTA ATGGGTAAAC AGGTACCATA CAAGAAGGCC AGAAGTGCAC 1321 CTCCTTTACT TAAAAGGAAA CCCCAGAGTG GATTATATGC GTCAGTTAGG AGCTCAGGCT 1381 ATGGCAAACC CAGTTCACCA CTCAAGATGT TTTCTACTCT TGAAAAGAAA ACTTCAGAGG 1441 ACATTATAAA AAGCAAAAAC TTGAGATCGA TTTCTACCTC CAATCAACCT AGGAAAAAAG 1501 AAATCTTATC TGGAACAAAA CTCATCAAGC CTGCAGCTTT GGATAAACCA GCTCACAAAA 1561 CTGAAAGTTG CCTGTCTACT CGTAAGAAGT CTGAAAATCC CACAGAAACT GATTCCTGTA 1621 TTCAGTTTCA GACTGTAGGA TTCCTTAGGA TACTGTGGTG CCCAACTTTC TTGTACAAAG 1681 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1741 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1801 ACGAAAAATG CGTTGGCGAT TGACGTTTAC GCGTTAAGTC gacaatcaac ctctggatta 1861 caaaatttgt gaaagatt