Construct: ORF TRCN0000470096
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017152.1_s317c1
- Derived from:
- ccsbBroadEn_08125
- DNA Barcode:
- GCTTAGCTGTGCTACTGTAGGAAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- REPIN1 (29803)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470096
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 29803 | REPIN1 | replication initiator 1 | NM_001099696.2 | 100% | 100% | |
| 2 | human | 29803 | REPIN1 | replication initiator 1 | NM_001362746.2 | 100% | 100% | |
| 3 | human | 29803 | REPIN1 | replication initiator 1 | NM_001362747.2 | 100% | 100% | |
| 4 | human | 29803 | REPIN1 | replication initiator 1 | NM_013400.3 | 100% | 100% | |
| 5 | human | 29803 | REPIN1 | replication initiator 1 | XM_006715952.2 | 100% | 100% | |
| 6 | human | 29803 | REPIN1 | replication initiator 1 | XM_017012081.2 | 100% | 100% | |
| 7 | human | 29803 | REPIN1 | replication initiator 1 | XM_017012082.1 | 100% | 100% | |
| 8 | human | 29803 | REPIN1 | replication initiator 1 | XM_024446737.1 | 100% | 100% | |
| 9 | human | 29803 | REPIN1 | replication initiator 1 | XM_024446738.1 | 100% | 100% | |
| 10 | human | 29803 | REPIN1 | replication initiator 1 | XM_006715950.3 | 91% | 91% | 1_168del |
| 11 | human | 29803 | REPIN1 | replication initiator 1 | XM_006715951.2 | 91% | 91% | 1_168del |
| 12 | human | 29803 | REPIN1 | replication initiator 1 | NM_001099695.2 | 90.8% | 90.8% | 1_171del |
| 13 | human | 29803 | REPIN1 | replication initiator 1 | NM_001362745.2 | 90.8% | 90.8% | 1_171del |
| 14 | human | 29803 | REPIN1 | replication initiator 1 | XM_006715947.3 | 90.8% | 90.8% | 1_171del |
| 15 | human | 29803 | REPIN1 | replication initiator 1 | XM_006715949.3 | 90.8% | 90.8% | 1_171del |
| 16 | mouse | 58887 | Repin1 | replication initiator 1 | NM_001079905.1 | 81.2% | 84.3% | (many diffs) |
| 17 | mouse | 58887 | Repin1 | replication initiator 1 | NM_175099.3 | 81.2% | 84.3% | (many diffs) |
| 18 | mouse | 58887 | Repin1 | replication initiator 1 | NM_001079902.1 | 74.4% | 77.2% | (many diffs) |
| 19 | mouse | 58887 | Repin1 | replication initiator 1 | NM_001079901.1 | 74.3% | 77% | (many diffs) |
| 20 | mouse | 58887 | Repin1 | replication initiator 1 | NM_001079904.1 | 74.1% | 76.8% | (many diffs) |
| 21 | mouse | 58887 | Repin1 | replication initiator 1 | NM_001079903.1 | 73.9% | 76.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1767
- ORF length:
- 1701
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct ggaacgtcgt tgcaggggcc ccctggccat gggcctggcc cagccccgac 121 tcctttctgg gccctcccag gagtcacccc agaccctggg gaaggagtcc cgcgggctga 181 ggcaacaagg cacgtcagtg gcccagtctg gtgcccaagc cccaggcagg gcccatcgct 241 gtgcccactg tcgaaggcac ttccctggct gggtggctct gtggcttcac acccgccggt 301 gccaggcccg gctgcccttg ccctgccctg agtgtggccg tcgctttcgc catgccccct 361 tcttagcact gcaccgccag gtccatgctg ctgccacccc agacctgggc tttgcctgcc 421 acctctgtgg gcagagcttc cgaggctggg tggccctggt tctgcatctg cgggcccatt 481 cagctgcaaa gcggcccatc gcttgtccca aatgcgagag acgcttctgg cgacgaaagc 541 agcttcgagc tcatctgcgg cggtgccacc ctcccgcccc ggaggcccgg cccttcatat 601 gcggcaactg tggccggagc tttgcccagt gggaccagct agttgcccac aagcgggtgc 661 acgtagctga ggccctggag gaggccgcag ccaaggctct ggggccccgg cccaggggcc 721 gccccgcggt gaccgccccc cggcccggtg gagatgccgt cgaccgcccc ttccagtgtg 781 cctgttgtgg caagcgcttc cggcacaagc ccaacttgat cgctcaccgc cgcgtgcaca 841 cgggcgagcg gccccaccag tgccccgagt gcgggaagcg ctttaccaat aagccctatc 901 tgacttcgca ccggcgcatc cacaccggcg agaagcccta cccgtgcaaa gagtgcggcc 961 gccgcttccg gcacaaaccc aacctgctgt ctcacagcaa gattcacaag cgatccgagg 1021 ggtcggccca ggccgccccc ggcccgggga gcccccagct gccagccggc ccccaggagt 1081 ccgcggccga gcccaccccg gcggtacctc tgaaaccggc ccaggagccg ccgccagggg 1141 ccccgccaga gcacccgcag gacccgatcg aagccccccc ctccctctac agctgcgacg 1201 actgcggcag gagcttccgg ctggagcgct tcctgcgggc ccaccagcgg cagcacaccg 1261 gggagcggcc cttcacctgc gccgagtgcg ggaagaactt cggcaagaag acgcacctgg 1321 tggcgcactc gcgcgtgcac tccggcgagc ggcccttcgc cTGCGAGGAG TGCGGCCGCC 1381 GCTTCTCCCA GGGCAGCCAT CTGGCGGCGC ATCGGCGCGA CCACGCCCCC GATCGGCCCT 1441 TCGTGTGTCC CGACTGCGGC AAGGCCTTCC GCCACAAACC CTACCTGGCG GCGCACCGGC 1501 GCATCCACAC CGGCGAGAAG CCCTACGTCT GCCCCGACTG CGGCAAAGCC TTCAGCCAGA 1561 AGTCCAACCT GGTGTCGCAC CGGCGCATCC ACACGGGCGA GCGGCCCTAC GCCTGTCCCG 1621 ACTGCGACCG CAGCTTCAGC CAGAAGTCCA ACCTCATCAC CCACCGCAAG AGCCACATCC 1681 GGGACGGCGC CTTCTGCTGT GCCATCTGTG GCCAGACCTT CGACGACGAG GAGAGACTCC 1741 TGGCCCACCA GAAGAAGCAC GATGTCTGCC CAACTTTCTT GTACAAAGTG GTTGATATCG 1801 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1861 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAGCTTAGCT 1921 GTGCTACTGT AGGAAAACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1981 aagatt