Construct: ORF TRCN0000470172
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002550.1_s317c1
- Derived from:
- ccsbBroadEn_00465
- DNA Barcode:
- TAGCTCTCCGCGAGATGAGTTAGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DTNB (1838)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470172
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1838 | DTNB | dystrobrevin beta | NM_183361.2 | 100% | 100% | |
| 2 | human | 1838 | DTNB | dystrobrevin beta | NM_033148.3 | 98.7% | 98.7% | 1465_1485del |
| 3 | human | 1838 | DTNB | dystrobrevin beta | NM_001351383.1 | 97.7% | 97.7% | 1366_1383del;1483_1503del |
| 4 | human | 1838 | DTNB | dystrobrevin beta | NM_001351386.1 | 96% | 92.6% | (many diffs) |
| 5 | human | 1838 | DTNB | dystrobrevin beta | NM_001351390.1 | 95.5% | 95.4% | 1465_1485del;1642_1643insAAGCAGAGG;1647_1648ins45 |
| 6 | human | 1838 | DTNB | dystrobrevin beta | NM_001351385.1 | 95% | 91.7% | (many diffs) |
| 7 | human | 1838 | DTNB | dystrobrevin beta | NM_001320933.1 | 94.9% | 94.9% | 1623_1712del |
| 8 | human | 1838 | DTNB | dystrobrevin beta | NM_001351382.1 | 94.9% | 91.5% | (many diffs) |
| 9 | human | 1838 | DTNB | dystrobrevin beta | XM_017003530.2 | 94% | 90.1% | (many diffs) |
| 10 | human | 1838 | DTNB | dystrobrevin beta | XM_017003529.1 | 93.9% | 93.9% | 1366_1383del;1641_1730del |
| 11 | human | 1838 | DTNB | dystrobrevin beta | NM_001351387.1 | 93.9% | 90.6% | (many diffs) |
| 12 | human | 1838 | DTNB | dystrobrevin beta | NM_001320934.1 | 93.8% | 93.8% | 1465_1485del;1644_1733del |
| 13 | human | 1838 | DTNB | dystrobrevin beta | NM_033147.3 | 93.8% | 93.6% | 1079_1168del;1555_1575del |
| 14 | human | 1838 | DTNB | dystrobrevin beta | NM_001351391.2 | 93.2% | 90.3% | (many diffs) |
| 15 | human | 1838 | DTNB | dystrobrevin beta | XM_011532676.1 | 92.8% | 92.8% | 1366_1383del;1483_1503del;1662_1751del |
| 16 | human | 1838 | DTNB | dystrobrevin beta | NM_001351384.1 | 91.8% | 91.5% | 1079_1168del;1711_1712insAAGCAGAGG;1716_1717ins45 |
| 17 | human | 1838 | DTNB | dystrobrevin beta | NM_001256304.2 | 91.3% | 87.9% | (many diffs) |
| 18 | human | 1838 | DTNB | dystrobrevin beta | NM_001351388.1 | 90.7% | 90.4% | (many diffs) |
| 19 | human | 1838 | DTNB | dystrobrevin beta | XM_011532675.1 | 90.4% | 87.1% | (many diffs) |
| 20 | human | 1838 | DTNB | dystrobrevin beta | NM_001256303.1 | 90.3% | 90.1% | 1079_1168del;1713_1802del |
| 21 | human | 1838 | DTNB | dystrobrevin beta | NM_183360.2 | 90.2% | 86.9% | (many diffs) |
| 22 | human | 1838 | DTNB | dystrobrevin beta | XM_011532673.1 | 89.4% | 89.2% | 1079_1168del;1456_1473del;1731_1820del |
| 23 | human | 1838 | DTNB | dystrobrevin beta | XM_011532674.1 | 89.3% | 86.1% | (many diffs) |
| 24 | human | 1838 | DTNB | dystrobrevin beta | NM_021907.4 | 89.3% | 89.1% | 1079_1168del;1555_1575del;1734_1823del |
| 25 | human | 1838 | DTNB | dystrobrevin beta | NM_001320936.1 | 88.5% | 84.8% | (many diffs) |
| 26 | human | 1838 | DTNB | dystrobrevin beta | XM_011532671.1 | 88.4% | 88.3% | (many diffs) |
| 27 | human | 1838 | DTNB | dystrobrevin beta | XM_011532672.2 | 87.6% | 84% | (many diffs) |
| 28 | human | 1838 | DTNB | dystrobrevin beta | NM_001351389.2 | 87.6% | 86.8% | (many diffs) |
| 29 | human | 1838 | DTNB | dystrobrevin beta | NM_001351394.1 | 85.5% | 78.1% | (many diffs) |
| 30 | human | 1838 | DTNB | dystrobrevin beta | XM_011532679.1 | 82.3% | 81.5% | (many diffs) |
| 31 | human | 1838 | DTNB | dystrobrevin beta | NM_001256308.2 | 81.8% | 78.7% | (many diffs) |
| 32 | human | 1838 | DTNB | dystrobrevin beta | XM_011532677.2 | 79.4% | 79.3% | (many diffs) |
| 33 | human | 1838 | DTNB | dystrobrevin beta | NM_001351393.1 | 72.6% | 72.6% | (many diffs) |
| 34 | human | 1838 | DTNB | dystrobrevin beta | XR_001738659.2 | 70.6% | 1_180del;1803_1978del;2037_2379del | |
| 35 | human | 1838 | DTNB | dystrobrevin beta | NM_001351392.1 | 69.5% | 66.6% | (many diffs) |
| 36 | human | 1838 | DTNB | dystrobrevin beta | NM_001351381.1 | 69% | 69% | (many diffs) |
| 37 | human | 1838 | DTNB | dystrobrevin beta | XM_017003539.2 | 68.3% | 68.3% | (many diffs) |
| 38 | human | 1838 | DTNB | dystrobrevin beta | NR_147180.1 | 67.6% | (many diffs) | |
| 39 | human | 1838 | DTNB | dystrobrevin beta | XR_939672.3 | 66.9% | (many diffs) | |
| 40 | human | 1838 | DTNB | dystrobrevin beta | XM_024452740.1 | 66.8% | 63.9% | (many diffs) |
| 41 | human | 1838 | DTNB | dystrobrevin beta | XM_017003538.2 | 66.4% | 66.2% | (many diffs) |
| 42 | human | 1838 | DTNB | dystrobrevin beta | XM_011532681.2 | 65.7% | 65.5% | (many diffs) |
| 43 | human | 1838 | DTNB | dystrobrevin beta | XM_011532680.3 | 65% | 64.9% | (many diffs) |
| 44 | human | 1838 | DTNB | dystrobrevin beta | XM_017003544.2 | 59.6% | 59.6% | 0_1ins612;853_873del;1032_1121del |
| 45 | human | 1838 | DTNB | dystrobrevin beta | XM_017003545.2 | 59.6% | 59.6% | 0_1ins612;853_873del;1032_1121del |
| 46 | human | 1838 | DTNB | dystrobrevin beta | XM_017003542.2 | 59% | 59% | (many diffs) |
| 47 | human | 1838 | DTNB | dystrobrevin beta | XM_017003543.2 | 59% | 59% | (many diffs) |
| 48 | human | 1838 | DTNB | dystrobrevin beta | NM_001320932.1 | 57.6% | 54.8% | (many diffs) |
| 49 | human | 1838 | DTNB | dystrobrevin beta | NM_001320935.1 | 57.4% | 57.2% | 0_1ins612;467_556del;1101_1190del |
| 50 | human | 1838 | DTNB | dystrobrevin beta | XM_024452743.1 | 56.7% | 56.6% | (many diffs) |
| 51 | human | 1838 | DTNB | dystrobrevin beta | XM_024452741.1 | 56.2% | 56% | (many diffs) |
| 52 | human | 1838 | DTNB | dystrobrevin beta | XM_024452742.1 | 56.2% | 56% | (many diffs) |
| 53 | human | 1838 | DTNB | dystrobrevin beta | NM_001320937.2 | 54.9% | 52.7% | (many diffs) |
| 54 | human | 1838 | DTNB | dystrobrevin beta | NM_001351395.1 | 43.3% | 33.6% | (many diffs) |
| 55 | mouse | 13528 | Dtnb | dystrobrevin, beta | XM_017314957.1 | 87.3% | 91.7% | (many diffs) |
| 56 | mouse | 13528 | Dtnb | dystrobrevin, beta | XM_017314955.1 | 87.3% | 91.8% | (many diffs) |
| 57 | mouse | 13528 | Dtnb | dystrobrevin, beta | XM_017314956.1 | 87.3% | 91.8% | (many diffs) |
| 58 | mouse | 13528 | Dtnb | dystrobrevin, beta | XM_017314954.1 | 83.9% | 88.1% | (many diffs) |
| 59 | mouse | 13528 | Dtnb | dystrobrevin, beta | NM_007886.2 | 83.5% | 84.5% | (many diffs) |
| 60 | mouse | 13528 | Dtnb | dystrobrevin, beta | XM_006514959.3 | 83.5% | 84.5% | (many diffs) |
| 61 | mouse | 13528 | Dtnb | dystrobrevin, beta | XM_006514960.3 | 83% | 87.1% | (many diffs) |
| 62 | mouse | 13528 | Dtnb | dystrobrevin, beta | XM_006514961.3 | 82.9% | 86.9% | (many diffs) |
| 63 | mouse | 13528 | Dtnb | dystrobrevin, beta | XM_017314953.1 | 82.8% | 85.9% | (many diffs) |
| 64 | mouse | 13528 | Dtnb | dystrobrevin, beta | XM_006514958.3 | 82.6% | 83.6% | (many diffs) |
| 65 | mouse | 13528 | Dtnb | dystrobrevin, beta | XM_017314952.1 | 81.9% | 85% | (many diffs) |
| 66 | mouse | 13528 | Dtnb | dystrobrevin, beta | XM_011243814.2 | 79% | 81.7% | (many diffs) |
| 67 | mouse | 13528 | Dtnb | dystrobrevin, beta | XM_011243813.2 | 78.1% | 80.9% | (many diffs) |
| 68 | mouse | 13528 | Dtnb | dystrobrevin, beta | XM_017314951.1 | 78.1% | 80.9% | (many diffs) |
| 69 | mouse | 13528 | Dtnb | dystrobrevin, beta | NM_001162465.1 | 76.8% | 80.2% | (many diffs) |
| 70 | mouse | 13528 | Dtnb | dystrobrevin, beta | XM_017314958.1 | 62.7% | 65.4% | (many diffs) |
| 71 | mouse | 13528 | Dtnb | dystrobrevin, beta | NR_027870.1 | 62.1% | (many diffs) | |
| 72 | mouse | 13528 | Dtnb | dystrobrevin, beta | NR_027869.1 | 61.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1746
- ORF length:
- 1680
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgat tgaggaaagt gggaacaagc ggaagaccat ggcagagaag aggcagctgt 121 tcatagaaat gcgtgctcag aattttgatg tcatacgact atcaacttac agaacagcct 181 gcaaattacg atttgtacaa aaacgatgca accttcatct tgttgatatc tggaacatga 241 ttgaagcctt ccgagacaat ggccttaata cactggacca taccaccgag atcagtgtgt 301 cccgcctcga aactgtcatc tcctccatct actatcagtt gaacaagcgc cttccttcta 361 ctcaccaaat tagtgtggaa caatctatca gcctcctcct caactttatg attgctgcat 421 atgacagtga gggccgaggc aagttgacgg tattttcagt taaagctatg ttagcaacca 481 tgtgtggtgg aaaaatgctg gacaaattga gatatgtttt ctcccagatg tcagattcca 541 atggcttaat gatatttagc aagtttgacc agtttctgaa ggaagttctg aagctcccaa 601 cagctgtctt tgaagggcca tcttttggtt acacagagca ctcagtccgc acctgttttc 661 cacagcagag aaagataatg ctaaatatgt ttttagacac aatgatggct gaccctcctc 721 cccagtgcct tgtctggcta cctctcatgc acaggcttgc ccatgttgag aatgtcttcc 781 atcccgtgga gtgctcctac tgccgatgtg agagtatgat gggtttccgg taccgatgcc 841 agcagtgcca caactatcag ctctgccaga attgcttttg gcgtggccat gccggcggcc 901 ctcacagcaa ccagcaccag atgaaggagc attcctcttg gaaatctcct gcaaagaagc 961 tgagccatgc aattagtaaa tctttggggt gtgtacccac gagagaaccc ccgcatcctg 1021 tttttcctga gcaaccagag aaaccacttg accttgcaca tatagttcct cctcgccctc 1081 tgactaatat gaatgacacc atggttagcc acatgtcctc tggagtgccc actcccacca 1141 agagtgttct ggacagtcct agccgactgg atgaggaaca ccgtcttata gctcgctatg 1201 ctgcccggct ggctgcagaa gcaggaaacg tgactcgtcc tcccactgac ttgagcttta 1261 actttgatgc caacaaacaa caaagacagc ttattgcaga actggaaaac aaaaacagag 1321 agatccTGCA GGAGATTCAG CGTCTCCGCC TGGAACACGA GCAGGCCTCC CAGCCCACCC 1381 CTGAGAAGGC ACAGCAGAAC CCCACGCTGC TGGCAGAGCT GCGGCTGCTG AGGCAAAGGA 1441 AGGATGAACT GGAGCAGAGG ATGTCGGCCC TGCAGGAGAG CAGGCGGGAG CTGATGGTCC 1501 AGCTGGAAGA GCTGATGAAG TTGCTGAAGG CTCAGGCCAC AGGGTCACCA CATACATCGC 1561 CCACCCATGG AGGCGGCCGG CCAATGCCCA TGCCAGTGCG CTCCACGTCT GCCGGCTCCA 1621 CCCCCACCCA CTGTCCGCAG GACTCGCTGA GCGGAGTCGG GGGAGACGTG CAGGAGGCCT 1681 TCGCACAAGC AGAGGAAGGT GCAGAGGAAG AAGAAGAGAA GATGCAGAAT GGGAAAGACA 1741 GAGGTTACCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1801 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1861 CTTGGCTTTA TATATCTTGT GGAAAGGACG ATAGCTCTCC GCGAGATGAG TTAGGACGCG 1921 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt