Construct: ORF TRCN0000470172

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF002550.1_s317c1
Derived from:
ccsbBroadEn_00465
DNA Barcode:
TAGCTCTCCGCGAGATGAGTTAGG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
DTNB (1838)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000470172
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 1838 DTNB dystrobrevin beta NM_183361.2 100% 100%
2 human 1838 DTNB dystrobrevin beta NM_033148.3 98.7% 98.7% 1465_1485del
3 human 1838 DTNB dystrobrevin beta NM_001351383.1 97.7% 97.7% 1366_1383del;1483_1503del
4 human 1838 DTNB dystrobrevin beta NM_001351386.1 96% 92.6% (many diffs)
5 human 1838 DTNB dystrobrevin beta NM_001351390.1 95.5% 95.4% 1465_1485del;1642_1643insAAGCAGAGG;1647_1648ins45
6 human 1838 DTNB dystrobrevin beta NM_001351385.1 95% 91.7% (many diffs)
7 human 1838 DTNB dystrobrevin beta NM_001320933.1 94.9% 94.9% 1623_1712del
8 human 1838 DTNB dystrobrevin beta NM_001351382.1 94.9% 91.5% (many diffs)
9 human 1838 DTNB dystrobrevin beta XM_017003530.2 94% 90.1% (many diffs)
10 human 1838 DTNB dystrobrevin beta XM_017003529.1 93.9% 93.9% 1366_1383del;1641_1730del
11 human 1838 DTNB dystrobrevin beta NM_001351387.1 93.9% 90.6% (many diffs)
12 human 1838 DTNB dystrobrevin beta NM_001320934.1 93.8% 93.8% 1465_1485del;1644_1733del
13 human 1838 DTNB dystrobrevin beta NM_033147.3 93.8% 93.6% 1079_1168del;1555_1575del
14 human 1838 DTNB dystrobrevin beta NM_001351391.2 93.2% 90.3% (many diffs)
15 human 1838 DTNB dystrobrevin beta XM_011532676.1 92.8% 92.8% 1366_1383del;1483_1503del;1662_1751del
16 human 1838 DTNB dystrobrevin beta NM_001351384.1 91.8% 91.5% 1079_1168del;1711_1712insAAGCAGAGG;1716_1717ins45
17 human 1838 DTNB dystrobrevin beta NM_001256304.2 91.3% 87.9% (many diffs)
18 human 1838 DTNB dystrobrevin beta NM_001351388.1 90.7% 90.4% (many diffs)
19 human 1838 DTNB dystrobrevin beta XM_011532675.1 90.4% 87.1% (many diffs)
20 human 1838 DTNB dystrobrevin beta NM_001256303.1 90.3% 90.1% 1079_1168del;1713_1802del
21 human 1838 DTNB dystrobrevin beta NM_183360.2 90.2% 86.9% (many diffs)
22 human 1838 DTNB dystrobrevin beta XM_011532673.1 89.4% 89.2% 1079_1168del;1456_1473del;1731_1820del
23 human 1838 DTNB dystrobrevin beta XM_011532674.1 89.3% 86.1% (many diffs)
24 human 1838 DTNB dystrobrevin beta NM_021907.4 89.3% 89.1% 1079_1168del;1555_1575del;1734_1823del
25 human 1838 DTNB dystrobrevin beta NM_001320936.1 88.5% 84.8% (many diffs)
26 human 1838 DTNB dystrobrevin beta XM_011532671.1 88.4% 88.3% (many diffs)
27 human 1838 DTNB dystrobrevin beta XM_011532672.2 87.6% 84% (many diffs)
28 human 1838 DTNB dystrobrevin beta NM_001351389.2 87.6% 86.8% (many diffs)
29 human 1838 DTNB dystrobrevin beta NM_001351394.1 85.5% 78.1% (many diffs)
30 human 1838 DTNB dystrobrevin beta XM_011532679.1 82.3% 81.5% (many diffs)
31 human 1838 DTNB dystrobrevin beta NM_001256308.2 81.8% 78.7% (many diffs)
32 human 1838 DTNB dystrobrevin beta XM_011532677.2 79.4% 79.3% (many diffs)
33 human 1838 DTNB dystrobrevin beta NM_001351393.1 72.6% 72.6% (many diffs)
34 human 1838 DTNB dystrobrevin beta XR_001738659.2 70.6% 1_180del;1803_1978del;2037_2379del
35 human 1838 DTNB dystrobrevin beta NM_001351392.1 69.5% 66.6% (many diffs)
36 human 1838 DTNB dystrobrevin beta NM_001351381.1 69% 69% (many diffs)
37 human 1838 DTNB dystrobrevin beta XM_017003539.2 68.3% 68.3% (many diffs)
38 human 1838 DTNB dystrobrevin beta NR_147180.1 67.6% (many diffs)
39 human 1838 DTNB dystrobrevin beta XR_939672.3 66.9% (many diffs)
40 human 1838 DTNB dystrobrevin beta XM_024452740.1 66.8% 63.9% (many diffs)
41 human 1838 DTNB dystrobrevin beta XM_017003538.2 66.4% 66.2% (many diffs)
42 human 1838 DTNB dystrobrevin beta XM_011532681.2 65.7% 65.5% (many diffs)
43 human 1838 DTNB dystrobrevin beta XM_011532680.3 65% 64.9% (many diffs)
44 human 1838 DTNB dystrobrevin beta XM_017003544.2 59.6% 59.6% 0_1ins612;853_873del;1032_1121del
45 human 1838 DTNB dystrobrevin beta XM_017003545.2 59.6% 59.6% 0_1ins612;853_873del;1032_1121del
46 human 1838 DTNB dystrobrevin beta XM_017003542.2 59% 59% (many diffs)
47 human 1838 DTNB dystrobrevin beta XM_017003543.2 59% 59% (many diffs)
48 human 1838 DTNB dystrobrevin beta NM_001320932.1 57.6% 54.8% (many diffs)
49 human 1838 DTNB dystrobrevin beta NM_001320935.1 57.4% 57.2% 0_1ins612;467_556del;1101_1190del
50 human 1838 DTNB dystrobrevin beta XM_024452743.1 56.7% 56.6% (many diffs)
51 human 1838 DTNB dystrobrevin beta XM_024452741.1 56.2% 56% (many diffs)
52 human 1838 DTNB dystrobrevin beta XM_024452742.1 56.2% 56% (many diffs)
53 human 1838 DTNB dystrobrevin beta NM_001320937.2 54.9% 52.7% (many diffs)
54 human 1838 DTNB dystrobrevin beta NM_001351395.1 43.3% 33.6% (many diffs)
55 mouse 13528 Dtnb dystrobrevin, beta XM_017314957.1 87.3% 91.7% (many diffs)
56 mouse 13528 Dtnb dystrobrevin, beta XM_017314955.1 87.3% 91.8% (many diffs)
57 mouse 13528 Dtnb dystrobrevin, beta XM_017314956.1 87.3% 91.8% (many diffs)
58 mouse 13528 Dtnb dystrobrevin, beta XM_017314954.1 83.9% 88.1% (many diffs)
59 mouse 13528 Dtnb dystrobrevin, beta NM_007886.2 83.5% 84.5% (many diffs)
60 mouse 13528 Dtnb dystrobrevin, beta XM_006514959.3 83.5% 84.5% (many diffs)
61 mouse 13528 Dtnb dystrobrevin, beta XM_006514960.3 83% 87.1% (many diffs)
62 mouse 13528 Dtnb dystrobrevin, beta XM_006514961.3 82.9% 86.9% (many diffs)
63 mouse 13528 Dtnb dystrobrevin, beta XM_017314953.1 82.8% 85.9% (many diffs)
64 mouse 13528 Dtnb dystrobrevin, beta XM_006514958.3 82.6% 83.6% (many diffs)
65 mouse 13528 Dtnb dystrobrevin, beta XM_017314952.1 81.9% 85% (many diffs)
66 mouse 13528 Dtnb dystrobrevin, beta XM_011243814.2 79% 81.7% (many diffs)
67 mouse 13528 Dtnb dystrobrevin, beta XM_011243813.2 78.1% 80.9% (many diffs)
68 mouse 13528 Dtnb dystrobrevin, beta XM_017314951.1 78.1% 80.9% (many diffs)
69 mouse 13528 Dtnb dystrobrevin, beta NM_001162465.1 76.8% 80.2% (many diffs)
70 mouse 13528 Dtnb dystrobrevin, beta XM_017314958.1 62.7% 65.4% (many diffs)
71 mouse 13528 Dtnb dystrobrevin, beta NR_027870.1 62.1% (many diffs)
72 mouse 13528 Dtnb dystrobrevin, beta NR_027869.1 61.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1746
ORF length:
1680
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgat tgaggaaagt gggaacaagc ggaagaccat ggcagagaag aggcagctgt
121tcatagaaat gcgtgctcag aattttgatg tcatacgact atcaacttac agaacagcct
181gcaaattacg atttgtacaa aaacgatgca accttcatct tgttgatatc tggaacatga
241ttgaagcctt ccgagacaat ggccttaata cactggacca taccaccgag atcagtgtgt
301cccgcctcga aactgtcatc tcctccatct actatcagtt gaacaagcgc cttccttcta
361ctcaccaaat tagtgtggaa caatctatca gcctcctcct caactttatg attgctgcat
421atgacagtga gggccgaggc aagttgacgg tattttcagt taaagctatg ttagcaacca
481tgtgtggtgg aaaaatgctg gacaaattga gatatgtttt ctcccagatg tcagattcca
541atggcttaat gatatttagc aagtttgacc agtttctgaa ggaagttctg aagctcccaa
601cagctgtctt tgaagggcca tcttttggtt acacagagca ctcagtccgc acctgttttc
661cacagcagag aaagataatg ctaaatatgt ttttagacac aatgatggct gaccctcctc
721cccagtgcct tgtctggcta cctctcatgc acaggcttgc ccatgttgag aatgtcttcc
781atcccgtgga gtgctcctac tgccgatgtg agagtatgat gggtttccgg taccgatgcc
841agcagtgcca caactatcag ctctgccaga attgcttttg gcgtggccat gccggcggcc
901ctcacagcaa ccagcaccag atgaaggagc attcctcttg gaaatctcct gcaaagaagc
961tgagccatgc aattagtaaa tctttggggt gtgtacccac gagagaaccc ccgcatcctg
1021tttttcctga gcaaccagag aaaccacttg accttgcaca tatagttcct cctcgccctc
1081tgactaatat gaatgacacc atggttagcc acatgtcctc tggagtgccc actcccacca
1141agagtgttct ggacagtcct agccgactgg atgaggaaca ccgtcttata gctcgctatg
1201ctgcccggct ggctgcagaa gcaggaaacg tgactcgtcc tcccactgac ttgagcttta
1261actttgatgc caacaaacaa caaagacagc ttattgcaga actggaaaac aaaaacagag
1321agatccTGCA GGAGATTCAG CGTCTCCGCC TGGAACACGA GCAGGCCTCC CAGCCCACCC
1381CTGAGAAGGC ACAGCAGAAC CCCACGCTGC TGGCAGAGCT GCGGCTGCTG AGGCAAAGGA
1441AGGATGAACT GGAGCAGAGG ATGTCGGCCC TGCAGGAGAG CAGGCGGGAG CTGATGGTCC
1501AGCTGGAAGA GCTGATGAAG TTGCTGAAGG CTCAGGCCAC AGGGTCACCA CATACATCGC
1561CCACCCATGG AGGCGGCCGG CCAATGCCCA TGCCAGTGCG CTCCACGTCT GCCGGCTCCA
1621CCCCCACCCA CTGTCCGCAG GACTCGCTGA GCGGAGTCGG GGGAGACGTG CAGGAGGCCT
1681TCGCACAAGC AGAGGAAGGT GCAGAGGAAG AAGAAGAGAA GATGCAGAAT GGGAAAGACA
1741GAGGTTACCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC
1801TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT
1861CTTGGCTTTA TATATCTTGT GGAAAGGACG ATAGCTCTCC GCGAGATGAG TTAGGACGCG
1921TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt

Download FASTA (ORF) (Full)