Construct: ORF TRCN0000470216

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF008049.1_s317c1
Derived from:
ccsbBroadEn_08219
DNA Barcode:
AGTTCCATTTCCCTCCTGACCCGT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MECR (51102)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000470216
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 51102 MECR mitochondrial trans-2-enoyl... NM_016011.5 99.8% 99.7% 286T>C;597A>G
2 human 51102 MECR mitochondrial trans-2-enoyl... NM_001349716.2 92.8% 92.7% 177_260del;370T>C;681A>G
3 human 51102 MECR mitochondrial trans-2-enoyl... NM_001349715.2 91.2% 90.9% 177_281del;391T>C;702A>G
4 human 51102 MECR mitochondrial trans-2-enoyl... NM_001349717.2 86.4% 84.1% (many diffs)
5 human 51102 MECR mitochondrial trans-2-enoyl... XM_011541540.2 86.3% 77.9% (many diffs)
6 human 51102 MECR mitochondrial trans-2-enoyl... XM_011541539.1 81.1% 73.2% (many diffs)
7 human 51102 MECR mitochondrial trans-2-enoyl... NM_001024732.4 79.4% 79.3% 0_1ins228;58T>C;369A>G
8 human 51102 MECR mitochondrial trans-2-enoyl... NM_001349711.2 79.4% 79.3% 0_1ins228;58T>C;369A>G
9 human 51102 MECR mitochondrial trans-2-enoyl... NM_001349712.2 79.4% 79.3% 0_1ins228;58T>C;369A>G
10 human 51102 MECR mitochondrial trans-2-enoyl... NM_001349713.2 79.4% 79.3% 0_1ins228;58T>C;369A>G
11 human 51102 MECR mitochondrial trans-2-enoyl... NM_001349714.2 79.4% 79.3% 0_1ins228;58T>C;369A>G
12 human 51102 MECR mitochondrial trans-2-enoyl... XM_017001413.1 79.4% 79.3% 0_1ins228;58T>C;369A>G
13 human 51102 MECR mitochondrial trans-2-enoyl... XM_024447442.1 79.4% 79.3% 0_1ins228;58T>C;369A>G
14 human 51102 MECR mitochondrial trans-2-enoyl... XM_024447443.1 79.4% 79.3% 0_1ins228;58T>C;369A>G
15 human 51102 MECR mitochondrial trans-2-enoyl... XM_024447444.1 79.4% 79.3% 0_1ins228;58T>C;369A>G
16 human 51102 MECR mitochondrial trans-2-enoyl... XM_024447446.1 79.4% 79.3% 0_1ins228;58T>C;369A>G
17 human 51102 MECR mitochondrial trans-2-enoyl... XM_024447448.1 79.4% 79.3% 0_1ins228;58T>C;369A>G
18 human 51102 MECR mitochondrial trans-2-enoyl... XM_024447450.1 79.4% 79.3% 0_1ins228;58T>C;369A>G
19 human 51102 MECR mitochondrial trans-2-enoyl... XM_011541541.1 79.3% 74.7% (many diffs)
20 human 51102 MECR mitochondrial trans-2-enoyl... XR_946663.2 70.9% (many diffs)
21 human 51102 MECR mitochondrial trans-2-enoyl... XM_011541543.1 68.7% 60.4% (many diffs)
22 human 51102 MECR mitochondrial trans-2-enoyl... XM_011541545.1 68.7% 60.4% (many diffs)
23 human 51102 MECR mitochondrial trans-2-enoyl... XM_011541546.1 68.7% 60.4% (many diffs)
24 human 51102 MECR mitochondrial trans-2-enoyl... XM_011541547.1 68.7% 60.4% (many diffs)
25 human 51102 MECR mitochondrial trans-2-enoyl... XM_011541549.1 68.7% 60.4% (many diffs)
26 human 51102 MECR mitochondrial trans-2-enoyl... XM_011541550.1 68.7% 60.4% (many diffs)
27 human 51102 MECR mitochondrial trans-2-enoyl... XM_011541553.1 68.7% 60.4% (many diffs)
28 human 51102 MECR mitochondrial trans-2-enoyl... XM_011541554.1 68.7% 60.4% (many diffs)
29 human 51102 MECR mitochondrial trans-2-enoyl... XM_017001411.1 68.7% 60.4% (many diffs)
30 human 51102 MECR mitochondrial trans-2-enoyl... XM_017001412.1 68.7% 60.4% (many diffs)
31 human 51102 MECR mitochondrial trans-2-enoyl... XR_001737209.1 66.4% (many diffs)
32 human 51102 MECR mitochondrial trans-2-enoyl... XM_017001416.1 65.2% 60.1% (many diffs)
33 human 51102 MECR mitochondrial trans-2-enoyl... XM_024447452.1 65.2% 60.1% (many diffs)
34 human 51102 MECR mitochondrial trans-2-enoyl... XR_002956765.1 61.4% (many diffs)
35 human 51102 MECR mitochondrial trans-2-enoyl... XR_001737210.1 57.3% (many diffs)
36 human 51102 MECR mitochondrial trans-2-enoyl... NR_146213.2 49.9% (many diffs)
37 human 51102 MECR mitochondrial trans-2-enoyl... NR_146215.2 47.2% (many diffs)
38 human 51102 MECR mitochondrial trans-2-enoyl... NR_146212.2 40.6% (many diffs)
39 human 51102 MECR mitochondrial trans-2-enoyl... NR_146214.2 35.3% (many diffs)
40 mouse 26922 Mecr mitochondrial trans-2-enoyl... XM_011250264.1 68.2% 69.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1185
ORF length:
1119
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgtg ggtctgcagt accctgtggc gggtgcgaac ccccgcccgg cagtggcggg
121ggctgctccc agcttctggc tgtcacggac ctgccgcctc ctcctactcc gcatccgccg
181agcctgcccg ggtccgggcg cttgtctatg ggcaccacgg ggatccagcc aaggtcgtcg
241aactcaagaa cctggagcta gctgctgtga gaggatcaga tgtccgtgtg aagatgctgg
301cggcccctat caatccatct gacataaata tgatccaagg aaactacgga ctccttcctg
361aactgcctgc tgttggaggg aacgaaggtg ttgcacaggt ggtagcggtg ggcagcaatg
421tgaccgggct gaagccagga gactgggtga ttccagcaaa tgctggttta ggaacctggc
481ggaccgaggc tgtgttcagc gaggaagcac tgatccaagt tccgagtgac atccctcttc
541agagcgctgc caccctgggt gtcaatccct gcacagccta caggatgttg atggacttcg
601agcaactgca gccaggggat tctgtcatcc agaatgcatc caacagcgga gtggggcaag
661cggtcatcca gatcgccgca gccctgggcc taagaaccat caatgtggtc cgagacagac
721cTGATATCCA GAAGCTGAGT GACAGACTGA AGAGTCTGGG GGCTGAGCAT GTCATCACAG
781AAGAGGAGCT AAGAAGGCCC GAAATGAAAA ACTTCTTTAA GGACATGCCC CAGCCACGGC
841TTGCTCTCAA CTGTGTTGGT GGGAAAAGCT CCACAGAGCT GCTGCGGCAG TTAGCGCGTG
901GAGGAACCAT GGTAACCTAT GGGGGGATGG CCAAGCAGCC CGTCGTAGCC TCTGTGAGCC
961TGCTCATTTT TAAGGATCTC AAACTTCGAG GCTTTTGGTT GTCCCAGTGG AAGAAGGATC
1021ACAGTCCAGA CCAGTTCAAG GAGCTGATCC TCACACTGTG CGATCTCATC CGCCGAGGCC
1081AGCTCACAGC CCCTGCCTGC TCCCAGGTCC CGCTGCAGGA CTACCAGTCT GCCTTGGAAG
1141CCTCCATGAA GCCCTTCATA TCTTCAAAGC AGATTCTCAC CATGTGCCCA ACTTTCTTGT
1201ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT
1261AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG
1321GAAAGGACGA AGTTCCATTT CCCTCCTGAC CCGTACGCGT TAAGTCgaca atcaacctct
1381ggattacaaa atttgtgaaa gatt

Download FASTA (ORF) (Full)