Construct: ORF TRCN0000470256
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002093.1_s317c1
- Derived from:
- ccsbBroadEn_02724
- DNA Barcode:
- TAGGAGACCTACACAGTATGTTGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SEPTIN6 (23157)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470256
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23157 | SEPTIN6 | septin 6 | NM_145802.4 | 100% | 100% | |
| 2 | human | 23157 | SEPTIN6 | septin 6 | NM_145799.4 | 99.4% | 99.5% | 1281_1281delTinsCTTTTTT |
| 3 | human | 23157 | SEPTIN6 | septin 6 | NM_145800.4 | 99.4% | 99.5% | 1281_1281delTinsCTTTTTT |
| 4 | human | 23157 | SEPTIN6 | septin 6 | XM_006724750.3 | 99.4% | 99.5% | 1281_1281delTinsCTTTTTT |
| 5 | human | 23157 | SEPTIN6 | septin 6 | XM_006724748.3 | 98.9% | 99% | 1280_1281insCTTT;1284_1293delGGGCTTCCTG |
| 6 | human | 23157 | SEPTIN6 | septin 6 | NM_015129.6 | 98.6% | 98.3% | (many diffs) |
| 7 | human | 23157 | SEPTIN6 | septin 6 | XM_011531318.3 | 91.4% | 91% | 1283C>T;1286_1289delACTG;1292_1407del |
| 8 | human | 23157 | SEPTIN6 | septin 6 | XM_005262400.4 | 87.3% | 86.9% | 1283C>T;1286_1289delACTG;1292_1473del |
| 9 | human | 23157 | SEPTIN6 | septin 6 | XM_011531317.3 | 81.4% | 81.1% | 1283C>T;1286_1289delACTG;1292_1578del |
| 10 | human | 23157 | SEPTIN6 | septin 6 | XR_001755677.2 | 46.7% | 1_91del;877_878ins169;1210_2221del | |
| 11 | human | 23157 | SEPTIN6 | septin 6 | XR_001755676.2 | 25.1% | (many diffs) | |
| 12 | human | 23157 | SEPTIN6 | septin 6 | XR_001755675.2 | 19.4% | (many diffs) | |
| 13 | mouse | 56526 | Sept6 | septin 6 | NM_019942.5 | 91.3% | 96.2% | (many diffs) |
| 14 | mouse | 56526 | Sept6 | septin 6 | NM_001177323.2 | 91% | 96% | (many diffs) |
| 15 | mouse | 56526 | Sept6 | septin 6 | NM_001253706.1 | 90.7% | 95.8% | (many diffs) |
| 16 | mouse | 56526 | Sept6 | septin 6 | XM_006541389.3 | 90.7% | 95.8% | (many diffs) |
| 17 | mouse | 56526 | Sept6 | septin 6 | XM_006541390.2 | 90.7% | 95.8% | (many diffs) |
| 18 | mouse | 56526 | Sept6 | septin 6 | XM_006541388.2 | 90.2% | 95.3% | (many diffs) |
| 19 | mouse | 56526 | Sept6 | septin 6 | NM_001177324.1 | 90% | 94.7% | (many diffs) |
| 20 | mouse | 56526 | Sept6 | septin 6 | XM_017318580.1 | 89.4% | 94.4% | (many diffs) |
| 21 | mouse | 56526 | Sept6 | septin 6 | XM_017318581.1 | 88.8% | 93.9% | (many diffs) |
| 22 | mouse | 56526 | Sept6 | septin 6 | XM_006541391.2 | 88.1% | 92.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1353
- ORF length:
- 1287
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc agcgaccgat atagctcgcc aggtgggtga aggttgccga actgtccccc 121 tggctggaca tgtggggttt gacagcttgc ctgaccagct ggtgaataag tccgtcagcc 181 agggcttctg cttcaacatc ctgtgcgtgg gagagacagg tttgggcaag tccaccctca 241 tggacaccct gttcaacacc aaattcgaag gggagccagc cacccacaca cagccgggtg 301 tccagctcca gtctaatacc tatgacctcc aagagagcaa cgtgaggcta aagctcacga 361 tcgttagcac agttggcttt ggggaccaga tcaacaaaga ggacagctac aagcctatcg 421 tggaattcat cgatgcacaa ttcgaggcct acctgcagga agagctaaag atccgaagag 481 tgctacacac ctaccatgac tcccgaatcc atgtctgctt gtatttcatt gcccccacgg 541 gtcattccct gaagtctctg gacctagtga ctatgaagaa gctggacagt aaggtgaaca 601 tcatccccat cattgccaaa gcagatgcca tttcgaagag tgagctaaca aagttcaaaa 661 tcaaaatcac cagcgagctt gtcagcaacg gagtccagat ctatcagttt cctacagatg 721 atgagtcggt ggcagagatc aatggaacca tgaacgccca cctgccgttt gctgtcattg 781 gcagcacaga agaactgaag ataggcaaca agatgatgag ggcgcggcag tatccttggg 841 gcactgtgca ggttgaaaac gaggcccact gcgactttgt gaagctgcgg gagatgctga 901 ttcgggtcaa catggaggat ctgcGGGAGC AGACCCACAC CCGGCACTAT GAGCTGTATC 961 GCCGCTGTAA GCTGGAGGAG ATGGGCTTCA AGGACACCGA CCCTGACAGC AAACCCTTCA 1021 GTTTACAGGA GACATATGAG GCCAAAAGGA ACGAGTTCCT AGGGGAACTC CAGAAAAAAG 1081 AAGAGGAGAT GAGACAGATG TTCGTCCAGC GAGTCAAAGA GAAAGAAGCG GAGCTCAAAG 1141 AGGCAGAGAA AGAGCTGCAC GAGAAGTTTG ACCGTCTGAA GAAACTGCAC CAGGACGAGA 1201 AGAAGAAACT GGAGGATAAG AAGAAATCCC TGGATGATGA AGTGAATGCT TTCAAGCAAA 1261 GAAAGACGGC GGCTGAGCTG CTCCAGTCCC AGGGCTCCCA GGCTGGAGGC TCACAGACTC 1321 TGAAGAGAGA CAAAGAGAAG AAAAACTTTT TTTACCCAAC TTTCTTGTAC AAAGTGGTTG 1381 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1441 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATA 1501 GGAGACCTAC ACAGTATGTT GAACGCGTTA AGTCgacaat caacctctgg attacaaaat 1561 ttgtgaaaga tt