Construct: ORF TRCN0000470266
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018923.3_s317c1
- Derived from:
- ccsbBroadEn_15251
- DNA Barcode:
- TGAGCCCCTGCAGCTATTTTACCA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- DGKK (139189)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470266
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 139189 | DGKK | diacylglycerol kinase kappa | NM_001013742.4 | 99.5% | 18.5% | (many diffs) |
| 2 | human | 139189 | DGKK | diacylglycerol kinase kappa | XM_017029268.2 | 97.2% | 19% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 882
- ORF length:
- 813
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggaccgcgga gctgccgcag cccagggcac tgccccgcct caggatggag 121 agcagcccgc tgagtctcca gagcctccgc cgccttggcc gccgccgcca ccaccaccgg 181 ctccgccgcc ggctccgccg ctgctctccg aggcttcgcc agaacccata ccagagccct 241 gtccagagct tgctccaggt ccctgtccag aggcgacctc agaatcagcc acagaactgt 301 acacagaacc gaccccagaa ccagccacag agccggcctc agaaccggcc ccagaacctg 361 ccacagagcc ggccccagaa cctgccacag agccggcccc agaaccggcc ccagaacctg 421 ccacagagtc tgccccagag ccgactccag aacctgccct agagtcggtc ccagagccgg 481 ccccagagct gactccagaa gttgccccag agctggcccc agagccgacc ccagaacctg 541 tgacagagct ggccccagag ttctgccctg aggcggctcc agagttccgt ccaagtccag 601 caccatgtct attgcaatgt ccggtggaca ctcgagagag aggtctaaag acctcgccat 661 cgccatcgcc atcgccatcg cccagaacgc caatgtcgtg gtccagaata aagaaaatat 721 tgaaggaagg acctatgctg aagaactgta actctttcag agatggaagc ttagatattt 781 tctggttcaa ggacagaagc tctactttgc acaccatccc gcgtttgcac actttgaaac 841 gattgatctg tctcaagcca ctggtggcag aaaggcagct gtagaaaccc tttggccaac 901 aggattgttg ttattacacc acaacgaaaa atcactctgg ctgcacccaa ccggaaagac 961 atggaagaat ggattaacat cataaaaacc atccaacagg gagaaattta taagatacct 1021 gcagcagaaa acaacccttt tcttgttgga atgcattgtt ggtactccag ttacagccac 1081 cggacccagc actgcaatgt ttgtcgagag agcattcctg ccttatctag agatgccatc 1141 atctgtgaag tgtgcaaagt gaaatctcac agattgtgtg ctttgagagc aagcaaagac 1201 tgcaagtgga atacattgtc tatcactgat gacctccttc tgcctgcaga tgaagtaaac 1261 atgccccatc aatgggtaga aggaaacatg cctgtcagct ctcagtgtgc agtgtgtcat 1321 gagagctgtg gcagttatca aagacttcaa gacttccgct gcctgtggtg taattctacg 1381 gtgcatgatg actgtaggag acggttttcc aaggaatgtt gcttcagaag ccatcgctca 1441 tcagtcattc ctcccactgc tctaagcgac cccaaaggcg atggccaatt agtagtatct 1501 tcagacttct ggaatcttga ttggtcatca gcctgttcat gtcccttgct catcttcatc 1561 aactccaaaa gtggcgatca tcaggggatc gtcttcctcc gaaaattcaa gcaatacctt 1621 aacccatctc aagtgttcga cttattgaag ggtggacctg aagcagggct gtctatgttc 1681 aagaactttg ctcgctttcg cattctggtt tgtggtggag atggcagcgt gagctgggtc 1741 ttatctctga ttgatgcctt tggattacat gaaaagtgtc agttggcagt catcccactt 1801 ggaaccggca atgatctggc tcgtgttctg ggctggggtg cattctggaa caaaagcaag 1861 tcacctctgg acatcctcaa cagagtggag caggctagtg tgaggatcct agacagatgg 1921 agtgtgatga ttcgtgagac tcccagacaa accccgctgc taaaaggaca ggttgaaatg 1981 gatgtaccac gatttgaggc tgctgccatc caacacttag aatctgcagc caccgagttg 2041 aacaaaatcc tgaaggccaa gtaccccaca gagatgatca tcgcaaccag attcttgtgt 2101 tcagctgtgg aagattttgt ggttgatatt gtaaaggcct ggggtcagat aaaacagaac 2161 aacactgcaa tagtgtctgt gattttgaaa agtgacttaa tgtatgatag gctcagtgtc 2221 ctgatcgatg tcctggctga ggaggcagca gctacttctg ctgaaaaaag tgctacagaa 2281 tatgcagaca gcagcaaggc agataggaag cccttcattc ctcaaataga ccacatagcc 2341 aagtgcaagt tggagctggc tacaaaggcc cagagtctcc agaaatcctt gaaactcatc 2401 atatttcaag ttgaacaagc tctggatgag gaaagcagac agacaatatc tgttaagaac 2461 tttagttcaa ctttcttcct ggaagatgac ccagaagata ttaaccagac aagcccacga 2521 cgccgttctc gtcgtggcac tttgtcttct atatcttctc tcaaaagtga ggacctggac 2581 aaccttaact tggatcactt acattttaca cctgaatcta tacgcttcaa agaaaaatgt 2641 gtcatgaaca actacttcgg aattggactg gatgctaaaa tttctctgga cttcaacacc 2701 agaagagatg aacacccagg gcaatacaat agccgcctta agaacaagat gtggtatggc 2761 cttctgggaa ccaaagaact tttgcagcgc tcttacagga aactggaaga acgagtgcat 2821 ttggagtgtg atggagaaac catctccttg ccaaacctgc aaggcattgt agtgctcaac 2881 attaccagct atgctggagg tatcaacttc tggggaagca acacagcaac cacggaatat 2941 gaggctcctg caatcgatga tgggaaactg gaggtggtgg caatctttgg ttctgtgcag 3001 atggcaatgt cccgtatcat caacctgcat catcatcgca ttgcccagtg ccatgaggtg 3061 atgataacca ttgattgtga agaaggtatc ccagggcagg tgatggggga ggcttggatt 3121 cagagaccag gctttatcaa aaatagatac aagaacgctg cccagatgct gacaagagat 3181 cgggactttg agaactcaat gaaaatgtgg gaatacaagc atactgaaat tcaagctgcc 3241 cctcaacccc agctggactt ccaggactct caagagagcc tctctgacga ggagtatgcc 3301 cagatgcagc acttagctcg gcttgcagaa aacctcatca gcaaacttaa tgacctgagc 3361 aagatccacc agcatgtgtc tgtcctcatg ggttctgtga atgccagcgc taacatcctg 3421 aataatatat tttacggcca agacagtggc aatgagatgg gtgcagcttc ctgtattcCC 3481 ATTGAAACTC TAAGCAGAAA TGATGCCGTA GATGTTACAT TTAGTCTTAA AGGTCTCTAC 3541 GATGACACCA CAGCTTTCCT GGATGAAAAG CTGCTGAGAA GTGCTGAGGA TGAGACTGCA 3601 CTACAAAGCG CCCTGGATGC CATGAATAAG GAGTTCAAAA AGCTATCTGA GATTGACTGG 3661 ATGAATCCAA TCTTTGTTCC AGAGGAAAAA TCTTCGGACA CTGACAGTAG AAGCCTCAGG 3721 CTGAAAATTA AGTTCCCCAA ATTGGGAAAG AAAAAGGTAG AAGAGGAACG CAAGCCTAAA 3781 TCAGGCCAGA GTGTCCAGAG TTTTATTGGC AATTTATGGC ACCGCAGACA TCGTGAAGAT 3841 GAAGCAGAGG GTGATGATCC TCTAACACCA TCGAGATCTC AACTGTTGCC AACTTTCTTG 3901 TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG 3961 TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT 4021 GGAAAGGACG ATGAGCCCCT GCAGCTATTT TACCAACGCG TTAAGTCgac aatcaacctc 4081 tggattacaa aatttgtgaa agatt