Construct: ORF TRCN0000470274

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF003264.1_s317c1
Derived from:
ccsbBroadEn_15371
DNA Barcode:
AATAGACTGCACTAGCTTCGTGAT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CA8 (767)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000470274
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 767 CA8 carbonic anhydrase 8 NM_001321837.2 99.8% 100% 327A>G
2 human 767 CA8 carbonic anhydrase 8 NM_004056.6 99.8% 100% 327A>G
3 human 767 CA8 carbonic anhydrase 8 NM_001321839.2 88.8% 88.9% 327A>G;415_416ins96
4 human 767 CA8 carbonic anhydrase 8 NM_001321838.2 88.5% 86.2% (many diffs)
5 human 767 CA8 carbonic anhydrase 8 XM_017013818.1 69% 67% (many diffs)
6 human 767 CA8 carbonic anhydrase 8 XM_011517588.3 53% 46.2% (many diffs)
7 human 767 CA8 carbonic anhydrase 8 XM_011517587.2 51.9% 48.4% (many diffs)
8 human 767 CA8 carbonic anhydrase 8 NR_135821.2 15% (many diffs)
9 mouse 12319 Car8 carbonic anhydrase 8 NM_007592.3 89.5% 98.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
936
ORF length:
870
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc ggacctgagc ttcatcgaag ataccgtcgc cttccccgag aaggaagagg
121atgaggagga agaagaggag ggtgtggagt ggggctacga ggaaggtgtt gagtggggtc
181tggtgtttcc tgatgctaat ggggaatacc agtctcctat taacctaaac tcaagagagg
241ctaggtatga cccctcgctg ttggatgtcc gcctctcccc aaattatgtg gtgtgccgag
301actgtgaagt caccaatgat ggacatacca ttcaggttat cctgaagtca aaatcagttc
361tttcgggagg accattgcct caagggcatg agtttgaact gtacgaagtg agatttcact
421ggggaagaga aaaccagcgt ggttctgagc acacggttaa tttcaaagct tttcccatgg
481agctccatct gatccactgg aactccactc tgtttggcag cattgatgag gctgtgggga
541agccgcacgg aatcgccatc attgctctgt ttgttcagat aggaaaggaa catgttggct
601tgaaggctgt gactgaaaTC CTCCAAGATA TTCAGTATAA GGGGAAGTCC AAAACAATAC
661CTTGCTTTAA TCCTAACACT TTATTACCAG ACCCTCTGCT GCGGGATTAC TGGGTGTATG
721AAGGCTCTCT CACCATCCCA CCTTGCAGTG AAGGTGTCAC CTGGATATTA TTCCGATACC
781CTTTAACTAT ATCCCAGCTA CAGATAGAAG AATTTCGAAG GCTGAGGACA CATGTTAAGG
841GGGCAGAACT TGTGGAAGGC TGTGATGGGA TTTTGGGAGA CAACTTTCGG CCCACTCAGC
901CTCTTAGTGA CAGAGTCATT AGAGCTGCAT TTCAGTACCC AACTTTCTTG TACAAAGTGG
961TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA
1021CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG
1081AAATAGACTG CACTAGCTTC GTGATACGCG TTAAGTCgac aatcaacctc tggattacaa
1141aatttgtgaa agatt

Download FASTA (ORF) (Full)