Construct: ORF TRCN0000470282
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006937.1_s317c1
- Derived from:
- ccsbBroadEn_08804
- DNA Barcode:
- TAGTGTCAACTCCATCTTCCCAAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CELF5 (60680)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470282
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 60680 | CELF5 | CUGBP Elav-like family memb... | NM_021938.4 | 99.9% | 100% | 159G>C |
| 2 | human | 60680 | CELF5 | CUGBP Elav-like family memb... | XM_006722832.1 | 99.9% | 100% | 159G>C |
| 3 | human | 60680 | CELF5 | CUGBP Elav-like family memb... | XM_006722833.1 | 99.7% | 99.7% | 159G>C;391_392insGTA |
| 4 | human | 60680 | CELF5 | CUGBP Elav-like family memb... | NM_001172673.2 | 84.2% | 75.1% | (many diffs) |
| 5 | human | 60680 | CELF5 | CUGBP Elav-like family memb... | XM_006722836.2 | 77.7% | 76.5% | (many diffs) |
| 6 | human | 60680 | CELF5 | CUGBP Elav-like family memb... | XR_001753733.1 | 76.7% | (many diffs) | |
| 7 | human | 60680 | CELF5 | CUGBP Elav-like family memb... | XM_006722837.4 | 76.4% | 76.4% | 0_1ins342 |
| 8 | human | 60680 | CELF5 | CUGBP Elav-like family memb... | XM_006722838.4 | 76.4% | 76.4% | 0_1ins342 |
| 9 | human | 60680 | CELF5 | CUGBP Elav-like family memb... | XM_011528173.1 | 76.4% | 76.4% | 0_1ins342 |
| 10 | human | 60680 | CELF5 | CUGBP Elav-like family memb... | XM_011528174.3 | 76.2% | 76.2% | 0_1ins342;49_50insGTA |
| 11 | human | 60680 | CELF5 | CUGBP Elav-like family memb... | XM_017027111.1 | 71.3% | 71.3% | 0_1ins417 |
| 12 | human | 60680 | CELF5 | CUGBP Elav-like family memb... | NR_033342.2 | 64.5% | (many diffs) | |
| 13 | human | 60680 | CELF5 | CUGBP Elav-like family memb... | XR_430147.1 | 39.3% | (many diffs) | |
| 14 | mouse | 319586 | Celf5 | CUGBP, Elav-like family mem... | XM_011243472.2 | 82.3% | 88.8% | (many diffs) |
| 15 | mouse | 319586 | Celf5 | CUGBP, Elav-like family mem... | XM_006513743.3 | 82.1% | 88.6% | (many diffs) |
| 16 | mouse | 319586 | Celf5 | CUGBP, Elav-like family mem... | NM_176954.3 | 72.9% | 69.9% | (many diffs) |
| 17 | mouse | 319586 | Celf5 | CUGBP, Elav-like family mem... | NM_001311124.1 | 72.7% | 69.7% | (many diffs) |
| 18 | mouse | 319586 | Celf5 | CUGBP, Elav-like family mem... | XM_006513744.3 | 72.6% | 74.7% | (many diffs) |
| 19 | mouse | 319586 | Celf5 | CUGBP, Elav-like family mem... | XM_006513749.3 | 68.3% | 74% | (many diffs) |
| 20 | mouse | 319586 | Celf5 | CUGBP, Elav-like family mem... | XM_006513747.1 | 68.2% | 74.2% | (many diffs) |
| 21 | mouse | 319586 | Celf5 | CUGBP, Elav-like family mem... | XM_006513748.3 | 68% | 74% | (many diffs) |
| 22 | mouse | 319586 | Celf5 | CUGBP, Elav-like family mem... | NM_001311127.1 | 58.9% | 55.6% | (many diffs) |
| 23 | mouse | 319586 | Celf5 | CUGBP, Elav-like family mem... | NM_001311126.1 | 58.7% | 55.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1521
- ORF length:
- 1455
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ccgcctgacg gagagcgagg cgcgccggca gcagcagcag ctcctgcagc 121 cgcggccctc gcccgtgggc agcagcgggc ccgagccccc cggggggcag cccgacggca 181 tgaaggacct ggacgccatc aaactcttcg tgggccagat cccccggcac ctggacgaga 241 aggacctcaa gccgctcttc gagcagttcg gccgcatcta cgagctcacg gtgctcaaag 301 acccctacac ggggatgcac aaaggctgtg ccttcctcac ctactgtgcc agggattccg 361 ccatcaaagc tcagactgcc ctgcacgagc agaagacctt gcccggaatg gcgcggccaa 421 tccaggtgaa gcctgcggac agtgaaagcc gcggaggtag ggaccggaag ctgttcgtgg 481 ggatgctgaa caagcagcag tcggaggagg acgtgctgcg gctgttccag cccttcgggg 541 tcattgacga gtgcaccgtg ctccgggggc ctgacggcag cagcaaaggc tgtgctttcg 601 tgaagttctc ctcccacacg gaggcgcagg cggccatcca cgccttgcat gggagccaga 661 ccatgccggg agcctcctcc agcctggtgg tcaagttcgc cgacacggac aaggagcgga 721 cgctccggcg catgcagcag atggtgggcc agctgggcat cctgacgccg tccctcacat 781 tgcccttcag cccctacagt gcctacgccc aggctctcat gcaacagcag acaacagtcc 841 tgtccacctc gggcagctac ctgagtcccg gcgtggcctt ctcaccctgt cacatccagc 901 agataggcgc cgtcagcctc aacgggctgc ctgccacacc catcgctcct gcctctgggc 961 tgcactcacc cccgctgctg ggcaccaccg ctgtgcctgg cctcgtggct cccatcacca 1021 atggctttgc aggtgtcgtg ccctttccag gtgggcaccc tgccctggaa accgtctatg 1081 ccaatggcct tgtgccctac ccagctcaga gcccgactgt ggccgagaca ctgcatcctg 1141 ccttctccgg agtccagcag tacacagcca tgtaccccac cgcggccatc acgcccatcg 1201 cgcacagcgT CCCCCAGCCG CCGCCCCTCC TGCAGCAGCA GCAGCGAGAA GGTCCCGAGG 1261 GCTGTAACCT GTTTATCTAC CACCTCCCCC AGGAGTTTGG AGACACGGAG CTGACGCAGA 1321 TGTTCCTACC CTTCGGCAAT ATCATTTCCT CCAAGGTGTT TATGGATCGA GCTACCAACC 1381 AGAGCAAGTG TTTCGGCTTC GTGAGCTTTG ATAACCCGGC CAGCGCCCAG GCAGCCATCC 1441 AGGCCATGAA CGGCTTCCAG ATCGGCATGA AGAGGCTCAA AGTCCAGCTG AAGCGGCCCA 1501 AAGACCCGGG ACACCCCTAC TGCCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1561 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1621 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGATAGT GTCAACTCCA 1681 TCTTCCCAAA ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt