Construct: ORF TRCN0000470316
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017202.1_s317c1
- Derived from:
- ccsbBroadEn_00216
- DNA Barcode:
- GCGTAGCCTTGGAGAACGACCAAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CAPN3 (825)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470316
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 825 | CAPN3 | calpain 3 | NM_173089.1 | 100% | 100% | |
| 2 | human | 825 | CAPN3 | calpain 3 | NM_173090.1 | 100% | 100% | |
| 3 | human | 825 | CAPN3 | calpain 3 | NM_173088.1 | 50.4% | 50.4% | 1_459del |
| 4 | human | 825 | CAPN3 | calpain 3 | NM_173087.1 | 21.3% | 21.3% | 1_1719del |
| 5 | human | 825 | CAPN3 | calpain 3 | NM_024344.1 | 19.1% | 19.1% | 1_1977del |
| 6 | human | 825 | CAPN3 | calpain 3 | NM_000070.3 | 19% | 19% | 1_1995del |
| 7 | mouse | 12335 | Capn3 | calpain 3 | NM_001109761.2 | 19.7% | 21% | (many diffs) |
| 8 | mouse | 12335 | Capn3 | calpain 3 | NM_001177799.1 | 19.2% | 20.4% | (many diffs) |
| 9 | mouse | 12335 | Capn3 | calpain 3 | NM_007601.3 | 17% | 18.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 534
- ORF length:
- 468
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gatctgtgca gatgagctca agaaggtcct taacacagtc gtgaacaaac 121 acaaggacct gaagacacac gggttcacac tggagtcctg ccgtagcatg attgcgctca 181 tggatacaga tggctctgga aagctcaacc TGCAGGAGTT CCACCACCTC TGGAACAAGA 241 TTAAGGCCTG GCAGAAAATT TTCAAACACT ATGACACAGA CCAGTCCGGC ACCATCAACA 301 GCTACGAGAT GCGAAATGCA GTCAACGACG CAGGATTCCA CCTCAACAAC CAGCTCTATG 361 ACATCATTAC CATGCGGTAC GCAGACAAAC ACATGAACAT CGACTTTGAC AGTTTCATCT 421 GCTGCTTCGT TAGGCTGGAG GGCATGTTCA GAGCTTTTCA TGCATTTGAC AAGGATGGAG 481 ATGGTATCAT CAAGCTCAAC GTTCTGGAGT GGCTGCAGCT CACCATGTAT GCCTGCCCAA 541 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 601 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 661 TATCTTGTGG AAAGGACGAG CGTAGCCTTG GAGAACGACC AACACGCGTT AAGTCgacaa 721 tcaacctctg gattacaaaa tttgtgaaag att