Construct: ORF TRCN0000470324
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001417.1_s317c1
- Derived from:
- ccsbBroadEn_08942
- DNA Barcode:
- CTAGTTCACTCCTCAGTACGAGAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BEND5 (79656)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470324
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79656 | BEND5 | BEN domain containing 5 | NM_024603.4 | 99.9% | 100% | 733C>A |
| 2 | human | 79656 | BEND5 | BEN domain containing 5 | XM_017002331.1 | 84% | 84% | (many diffs) |
| 3 | human | 79656 | BEND5 | BEN domain containing 5 | NM_001349795.1 | 79.7% | 79.8% | 0_1ins255;478C>A |
| 4 | human | 79656 | BEND5 | BEN domain containing 5 | XM_011542141.3 | 79.7% | 79.8% | 0_1ins255;478C>A |
| 5 | human | 79656 | BEND5 | BEN domain containing 5 | XM_017002333.2 | 62.2% | 59.8% | (many diffs) |
| 6 | human | 79656 | BEND5 | BEN domain containing 5 | NR_146232.1 | 60.1% | (many diffs) | |
| 7 | human | 79656 | BEND5 | BEN domain containing 5 | NM_001302082.1 | 59.7% | 59.8% | 0_1ins507;226C>A |
| 8 | human | 79656 | BEND5 | BEN domain containing 5 | NM_001349793.1 | 59.7% | 59.8% | 0_1ins507;226C>A |
| 9 | human | 79656 | BEND5 | BEN domain containing 5 | NM_001349794.1 | 59.7% | 59.8% | 0_1ins507;226C>A |
| 10 | human | 79656 | BEND5 | BEN domain containing 5 | XM_011542142.2 | 59.7% | 59.8% | 0_1ins507;226C>A |
| 11 | human | 79656 | BEND5 | BEN domain containing 5 | XR_001737408.2 | 57.2% | (many diffs) | |
| 12 | mouse | 67621 | Bend5 | BEN domain containing 5 | NM_026279.3 | 94.3% | 98.5% | (many diffs) |
| 13 | mouse | 67621 | Bend5 | BEN domain containing 5 | XM_006503323.3 | 66.8% | 70% | (many diffs) |
| 14 | mouse | 67621 | Bend5 | BEN domain containing 5 | NR_033793.1 | 62.4% | (many diffs) | |
| 15 | mouse | 67621 | Bend5 | BEN domain containing 5 | XM_011240594.2 | 56.7% | 59.6% | (many diffs) |
| 16 | mouse | 67621 | Bend5 | BEN domain containing 5 | XM_017320366.1 | 56.7% | 59.6% | (many diffs) |
| 17 | mouse | 67621 | Bend5 | BEN domain containing 5 | XR_376343.3 | 34.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1329
- ORF length:
- 1263
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgta cgcctttgtg cggttcctgg aggacaacgt ctgctacgcg ctgcccgtgt 121 cgtgcgtgcg cgacttcagc ccccgctcgc ggctggattt tgacaaccag aaggtgtacg 181 ccgtgtaccg gggcccggag gaattgggcg ccgggcccga gagccccccg cgcgcccccc 241 gcgactgggg cgcgctgttg ctccacaagg cccagatcct ggcgctggca gaagacaaat 301 ctgaccttga aaacagtgtg atgcagaaga aaataaaaat ccccaagctt tctcttaatc 361 atgtagaaga agatggagag gttaaagatt atggggaaga agatttacag cttagacaca 421 tcaagagacc tgaggggcgg aagccgagcg aagtggcgca caagagcatc gaggcagtgg 481 tggctcggct agagaagcag aacggcctga gcctgggcca tagcacgtgt ccggaagagg 541 tcttcgtgga ggcctcgcca ggcacagagg acatggacag tctagaagat gctgtggtgc 601 cccgggctct gtatgaggag ctgctgcgca actaccagca gcaacaggaa gagatgcgcc 661 acctccagca ggagctggag cggactcgga ggcagctggt acaacaggcc aagaagctca 721 aggagtacgg ggcacttgtg tctgaaatga aggagctccg tgaccttaac cggaggctcc 781 aggacgtgct gctcctgagg cttggcagcg gtcccgccat tgatctggaa aaagtaaagt 841 cagaatgtct cgagcccgag ccggagttac ggagcacttt cagtgaggaa gcaaatacgt 901 cgtcctatta ccccgctcct gcgcctgtca tggacaagta tatcctagac aatggcaagg 961 tccatctggg AAGCGGGATT TGGGTTGATG AGGAGAAATG GCACCAGCTA CAAGTAACCC 1021 AAGGAGATTC CAAGTACACG AAGAACTTGG CAGTTATGAT TTGGGGAACA GATGTTCTGA 1081 AAAACAGAAG CGTCACAGGC GTCGCCACAA AAAAAAAGAA AGATGCAGTC CCTAAACCAC 1141 CCCTCTCGCC TCACAAACTA AGCATCGTCA GAGAGTGTTT GTATGACAGA ATAGCACAAG 1201 AAACTGTGGA TGAAACTGAA ATTGCACAGA GACTCTCCAA AGTCAACAAG TACATCTGTG 1261 AAAAAATCAT GGATATCAAT AAATCCTGTA AAAATGAAGA ACGAAGGGAA GCAAAATACA 1321 ATTTGCAATA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1381 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1441 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGACTAGTT CACTCCTCAG TACGAGATAC 1501 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt