Construct: ORF TRCN0000470343
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010675.1_s317c1
- Derived from:
- ccsbBroadEn_03790
- DNA Barcode:
- AGGAACAGCCGACTATTCGATCCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CHMP1B (57132)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470343
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57132 | CHMP1B | charged multivesicular body... | NM_020412.5 | 100% | 100% | |
| 2 | mouse | 67064 | Chmp1b | charged multivesicular body... | NM_024190.2 | 92.2% | 97.9% | (many diffs) |
| 3 | mouse | 67028 | 2610002M06Rik | RIKEN cDNA 2610002M06 gene | NM_025921.3 | 82% | 95.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 663
- ORF length:
- 597
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc taacatggag aaacacctgt tcaacctgaa gttcgcggcc aaagaactga 121 gtaggagtgc caaaaaatgc gataaggagg aaaaggccga aaaggccaaa attaaaaagg 181 ccattcagaa gggcaacatg gaagttgcga ggatacacgc cgaaaatgcc aTCCGCCAGA 241 AGAACCAGGC GGTGAATTTC TTGAGAATGA GTGCGCGAGT CGATGCAGTG GCTGCCAGGG 301 TCCAGACGGC GGTGACGATG GGCAAGGTGA CCAAGTCGAT GGCTGGTGTG GTTAAGTCGA 361 TGGATGCGAC ATTGAAGACC ATGAATCTGG AGAAGATTTC TGCTTTGATG GACAAATTCG 421 AGCACCAGTT TGAGACTCTG GACGTCCAGA CGCAGCAAAT GGAAGACACG ATGAGCAGCA 481 CGACGACGCT CACCACTCCC CAGAACCAAG TGGATATGCT GCTCCAGGAA ATGGCAGATG 541 AGGCGGGCCT CGACCTCAAC ATGGAGCTGC CGCAGGGCCA GACCGGCTCC GTGGGCACGA 601 GCGTGGCTTC GGCGGAGCAG GATGAACTGT CTCAGAGACT GGCCCGCCTT CGGGATCAAG 661 TGTGCCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 721 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 781 GGCTTTATAT ATCTTGTGGA AAGGACGAAG GAACAGCCGA CTATTCGATC CGACGCGTTA 841 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt