Construct: ORF TRCN0000470354
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010749.1_s317c1
- Derived from:
- ccsbBroadEn_09706
- DNA Barcode:
- AAGCATGACCCCTATGGCTCGTTA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- SAMD3 (154075)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470354
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 154075 | SAMD3 | sterile alpha motif domain ... | XM_017010310.2 | 87.3% | 32.1% | (many diffs) |
| 2 | human | 154075 | SAMD3 | sterile alpha motif domain ... | XM_017010309.2 | 85.2% | 31% | (many diffs) |
| 3 | human | 154075 | SAMD3 | sterile alpha motif domain ... | XM_017010308.2 | 58.4% | 21.3% | (many diffs) |
| 4 | human | 154075 | SAMD3 | sterile alpha motif domain ... | NM_001017373.4 | 42% | 15.3% | (many diffs) |
| 5 | human | 154075 | SAMD3 | sterile alpha motif domain ... | NM_001258275.3 | 42% | 15.3% | (many diffs) |
| 6 | human | 154075 | SAMD3 | sterile alpha motif domain ... | XM_024446334.1 | 42% | 15.3% | (many diffs) |
| 7 | human | 154075 | SAMD3 | sterile alpha motif domain ... | XM_024446335.1 | 42% | 15.3% | (many diffs) |
| 8 | human | 154075 | SAMD3 | sterile alpha motif domain ... | XM_024446336.1 | 42% | 15.3% | (many diffs) |
| 9 | human | 154075 | SAMD3 | sterile alpha motif domain ... | XM_024446337.1 | 42% | 15.3% | (many diffs) |
| 10 | human | 154075 | SAMD3 | sterile alpha motif domain ... | XM_017010307.1 | 41.9% | 15.4% | (many diffs) |
| 11 | human | 154075 | SAMD3 | sterile alpha motif domain ... | XM_024446333.1 | 40.5% | 14.8% | (many diffs) |
| 12 | human | 154075 | SAMD3 | sterile alpha motif domain ... | XM_017010305.1 | 40.3% | 14.8% | (many diffs) |
| 13 | human | 154075 | SAMD3 | sterile alpha motif domain ... | NM_001277185.1 | 40.2% | 14.7% | (many diffs) |
| 14 | human | 154075 | SAMD3 | sterile alpha motif domain ... | XR_001743174.2 | 34.6% | (many diffs) | |
| 15 | human | 154075 | SAMD3 | sterile alpha motif domain ... | XR_001743172.2 | 33.9% | (many diffs) | |
| 16 | human | 154075 | SAMD3 | sterile alpha motif domain ... | XR_001743171.2 | 30.8% | (many diffs) | |
| 17 | human | 154075 | SAMD3 | sterile alpha motif domain ... | XR_001743173.2 | 23.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 345
- ORF length:
- 279
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga aacctggtca gttgagcagg tctgcagttg gttggtggag aaaaatttag 121 gagagctagt tcatagattt caagaggaag aagtaagtgg ggccgctctt cttgcactta 181 atgatcggat ggttcagcaa ctgGTAAAGA AAATTGGGCA CCAGGCTGTT CTGATGGATT 241 TAATTAAAAA ATACAAGCAG AACACTCAAG GACTGAAGTC CCCCAGAAAA CCCCAAAAAG 301 GCAGCCCTGG TCATGCAAAC AGAAGCAGCT CGAGATTACA GGGATAAAGA GTCCTCCAGT 361 CCAGCCAGGC ATGGGGAGCA GATGCCATCT TTCTATCCAG CTGAAAACCT TGATAATGGA 421 CTAATTGACC AAAGAGTATT GAAACAGAGA AGAAATGTGA AACAAATTCT AGCAAGAAGC 481 AAAGCATTAC AGTGGACGAA GTCCTATGTT TTACCAGAGT TTCCCTATGA TGTCAAATGC 541 ATGTTAGCAG AGCAGAAGTG CCCGGATCAC AGCATGAGGA TAAGGATCAT TGAGTTTCTC 601 CAGGCCGACA TGACTAAGTA TCTGGAAGGC TCACTGTACC CCAGCACCCA GCAGTACAAT 661 GACGTGGTTA ATGCCCTGCT GCAGGCCCAC CCTTTCCTGG ATGAGGATGG CTGTGGCTTC 721 GCTGGAGTAT ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC 781 CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG 841 ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAAAGCA TGACCCCTAT GGCTCGTTAA 901 CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg tgaaagatt