Construct: ORF TRCN0000470356
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003657.1_s317c1
- Derived from:
- ccsbBroadEn_06702
- DNA Barcode:
- GTTAATAAGAAGTCGGGGCTCCGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PDE3B (5140)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470356
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5140 | PDE3B | phosphodiesterase 3B | NM_000922.4 | 99.8% | 99.9% | (many diffs) |
| 2 | human | 5140 | PDE3B | phosphodiesterase 3B | XM_017017911.2 | 96.4% | 96.4% | (many diffs) |
| 3 | human | 5140 | PDE3B | phosphodiesterase 3B | NM_001363569.1 | 95.2% | 95.3% | (many diffs) |
| 4 | human | 5140 | PDE3B | phosphodiesterase 3B | NM_001363570.1 | 93.3% | 93.3% | (many diffs) |
| 5 | human | 5140 | PDE3B | phosphodiesterase 3B | XM_017017912.1 | 86.4% | 86.5% | (many diffs) |
| 6 | human | 5140 | PDE3B | phosphodiesterase 3B | XM_006718249.3 | 65.9% | 66% | (many diffs) |
| 7 | human | 5140 | PDE3B | phosphodiesterase 3B | XR_001747903.2 | 54.1% | (many diffs) | |
| 8 | mouse | 18576 | Pde3b | phosphodiesterase 3B, cGMP-... | NM_011055.2 | 83% | 80.1% | (many diffs) |
| 9 | mouse | 18576 | Pde3b | phosphodiesterase 3B, cGMP-... | XM_006507438.3 | 82% | 79.2% | (many diffs) |
| 10 | mouse | 18576 | Pde3b | phosphodiesterase 3B, cGMP-... | XR_001785491.1 | 34.6% | (many diffs) | |
| 11 | mouse | 18576 | Pde3b | phosphodiesterase 3B, cGMP-... | XR_001785490.1 | 32.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3405
- ORF length:
- 3336
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaggagggac gagcgagacg ccaaagccat gcggtccctg cagccgccgg 121 atggggccgg ctcgcccccc gagagtctga ggaacggcta cgtgaagagc tgcgtgagcc 181 ccttgcggca ggaccctccg cgcggcttct tcttccacct ctgccacttc tgcaacgtgg 241 agctgcggcc gccgccggcc tctccccagc agccgcggcg ctgctccccc ttctgccggg 301 cgcgcctctc gctgggcgcc ctggctgcct ttgtcctcgc cctgctgctg ggcgcggaac 361 ccgagagctg ggctgccggg gccgcctggc tgcggacgct gctgagcgtg tgttcgcaca 421 gcttgagccc cctcttcagc atcgcctgtg ccttcttctt cctcacctgc ttcctcaccc 481 ggaccaagcg gggacccggc ccgggccgga gctgcggctc ctggtggctg ctggcgctgc 541 ccgcctgctg ttacctgggg gacttcttgg tgtggcagtg gtggtcttgg ccttgggggg 601 atggcgacgc agggtccgcg gccccgcaca cgcccccgga ggcggcagcg ggcaggttgc 661 tgctggtgct gagctgcgta gggctgctgc tgacgctcgc gcacccgctg cggctgcggc 721 actgcgttct ggtgctgctc ctggccagct tcgtctggtg ggtctccttc accagcctcg 781 ggtcgctgcc ctccgccctc aggccgctgc tctccggcct ggtggggggc gctggctgcc 841 tgctggccct ggggttggat cacttctttc aaatcaggga agcgcctctt catcctcgac 901 tgtccagtgc cgccgaagaa aaagtgcctg tgatccgacc ccggaggagg tccagctgcg 961 tgtcgttagg agaaactgca gccagttact atggcagttg caaaatattc aggagaccgt 1021 cgttgccttg tatttccaga gaacagatga ttctttggga ttgggactta aaacaatggt 1081 ataagcctca ttatcaaaat tctggaggtg gaaatggagt tgatctttca gtgctaaatg 1141 aggctcgcaa tatggtgtca gatcttctga ctgatccaag ccttccacca caagtcattt 1201 cctctctacg gagtattagt agcttaatgg gtgctttctc aggttcctgt aggccaaaga 1261 ttaatcctct cacaccattt cctggatttt acccctgttc tgaaatagag gacccagctg 1321 agaaagggga tagaaaactt aacaagggac taaataggaa tagtttgcca actccacagc 1381 tgaggagaag ctcgggaact tcaggattgc tacctgttga acagtcttca aggtgggatc 1441 gtaataatgg caaaaggcct caccaagaat ttggcatttc aagtcaagga tgctatctaa 1501 atgggccttt taattcaaat ctactgacta tcccgaagca aaggtcatct tctgtatcac 1561 tgactcacca tgtaggtctc agaagagctg gtgttttgtc cagtctgagt cctgtgaatt 1621 cttccaacca tggaccagtg tctactggct ctctaactaa tcgatcaccc atagaatttc 1681 ctgatactgc tgattttctt aataagccaa gcgttatctt gcagagatct ctgggcaatg 1741 cacctaatac tccagatttt tatcagcaac ttagaaattc tgatagcaat ctgtgtaaca 1801 gctgtggaca tcaaatgctg aaatatgttt caacatctga atcagatggt acagattgct 1861 gcagtggaaa atcaggtgaa gaagaaaaca ttttctcgaa agaatcattc aaacttatgg 1921 aaactcaaca agaagaggaa acagagaaga aagacagcag aaaattattt caggaaggtg 1981 ataagtggct aacagaagag gcacagagtg aacagcaaac aaatattgaa caggaagtat 2041 cactggacct gattttagta gaagagtatg actcattaat agaaaagatg agcaactgga 2101 attttccaat ttttgaactt gtagaaaaga tgggagagaa atcaggaagg attctcagtc 2161 aggttatgta taccttattt caagacactg gtttattgga aatatttaaa attcccactc 2221 aacaatttat gaactatttt cgtgcattag aaaatggcta tcgagacatt ccttatcaca 2281 atcgtataca tgccacagat gtgctacatg cagtttggta tctgacaaca cggccagttc 2341 ctggcttaca gcagatccac aatggttgtg gaacaggaaa tgaaacagat tctgatggta 2401 gaattaacca tgggcgaatt gcttatattt cttcgaagag ctgctctaat cctgatgaga 2461 gttatggctg cctgtcttca aacattcctg cattagaatt gatggctcta tacgtggcag 2521 ctgccatgca tgattatgat cacccaggga ggacaaatgc atttctagtg gctacaaatg 2581 cccctcaggc agttttatac aatgacagat ctgttctgga aaatcatcat gctgcgtcag 2641 cttggaatct atatctttct cgcccagaat acaacttcct tcttcatctt gatcatgtgg 2701 aattcaagcg ctttcgtttt ttagtcattg aagcaatcct tgctacggat cttaaaaagc 2761 attttgattt tctcgcagaa ttcaatgcca aggcaaatga tgtaaatagt aatggcatag 2821 aatggagtaa tgaaaatgat cgcctcttgg tatgccaggt gtgcatcaaa ctggcagata 2881 taaatggccc agcaaaagtt cgagacttgc atttgaaatg gacagaaggc attgtcaatg 2941 aattttatga gcagggagat gaagaagcaa atcttggtct gcccatcagt ccattcatgg 3001 atcgttcttc tcctcaacta gcaaaactcc aagaaTCTTT TATCACCCAC ATAGTGGGTC 3061 CCCTGTGTAA CTCCTATGAT GCTGCTGGTT TGCTACCAGG TCAGTGGTTA GAAGCAGAAG 3121 AGGATAATGA TACTGAAAGT GGTGATGATG AAGACGGTGA AGAATTAGAT ACAGAAGATG 3181 AAGAAATGGA AAACAATCTA AATCCAAAAC CACCAAGAAG GAAAAGCAGA CGGCGAATAT 3241 TTTGTCAGCT AATGCACCAC CTCACTGAAA ACCACAAGAT ATGGAAGGAA ATCGTAGAGG 3301 AAGAAGAAAA ATGTAAAGCT GATGGGAATA AACTGCAGGT GGAGAATTCC TCCTTACCTC 3361 AAGCAGATGA GATTCAGGTA ATTGAAGAGG CAGATGAAGA GGAATTGCCA ACTTTCTTGT 3421 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 3481 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 3541 GAAAGGACGA GTTAATAAGA AGTCGGGGCT CCGAACGCGT TAAGTCgaca atcaacctct 3601 ggattacaaa atttgtgaaa gatt