Construct: ORF TRCN0000470372
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009864.1_s317c1
- Derived from:
- ccsbBroadEn_07561
- DNA Barcode:
- CGGTATTACTATGAGTGCGCAACC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- YAF2 (10138)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470372
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10138 | YAF2 | YY1 associated factor 2 | NM_001190979.2 | 99.8% | 100% | 501T>C |
| 2 | human | 10138 | YAF2 | YY1 associated factor 2 | NM_005748.5 | 88% | 88.2% | 151_152ins72;429T>C |
| 3 | human | 10138 | YAF2 | YY1 associated factor 2 | XM_011537728.3 | 85.5% | 85.7% | 152_253del;603T>C |
| 4 | human | 10138 | YAF2 | YY1 associated factor 2 | XM_024448792.1 | 84.8% | 71.1% | (many diffs) |
| 5 | human | 10138 | YAF2 | YY1 associated factor 2 | XM_006719185.3 | 82.6% | 73.1% | (many diffs) |
| 6 | human | 10138 | YAF2 | YY1 associated factor 2 | XM_011537729.2 | 77.2% | 75.9% | (many diffs) |
| 7 | human | 10138 | YAF2 | YY1 associated factor 2 | NM_001320080.1 | 69.2% | 57.5% | (many diffs) |
| 8 | human | 10138 | YAF2 | YY1 associated factor 2 | NM_001190977.2 | 67.4% | 67.6% | 24_25ins198;303T>C |
| 9 | human | 10138 | YAF2 | YY1 associated factor 2 | XM_017018670.2 | 34.8% | 31.3% | (many diffs) |
| 10 | human | 10138 | YAF2 | YY1 associated factor 2 | XR_245886.4 | 23.4% | (many diffs) | |
| 11 | human | 10138 | YAF2 | YY1 associated factor 2 | XR_001748538.1 | 22.4% | (many diffs) | |
| 12 | human | 10138 | YAF2 | YY1 associated factor 2 | XR_002957280.1 | 21.9% | (many diffs) | |
| 13 | human | 10138 | YAF2 | YY1 associated factor 2 | XR_001748537.1 | 21.4% | (many diffs) | |
| 14 | human | 10138 | YAF2 | YY1 associated factor 2 | NR_135139.1 | 13.7% | (many diffs) | |
| 15 | human | 10138 | YAF2 | YY1 associated factor 2 | NR_135140.1 | 13.6% | (many diffs) | |
| 16 | mouse | 67057 | Yaf2 | YY1 associated factor 2 | XM_006521292.2 | 84.3% | 87.2% | (many diffs) |
| 17 | mouse | 67057 | Yaf2 | YY1 associated factor 2 | NM_024189.6 | 75.4% | 80.8% | (many diffs) |
| 18 | mouse | 67057 | Yaf2 | YY1 associated factor 2 | NR_028315.1 | 22.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 678
- ORF length:
- 612
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg agacaagaag agccccacca ggccgaagcg gcagccgaag ccgtcctcgg 121 atgagggtta ctgggactgt agcgtctgca ccttccggaa cagcgccgag gccttcaagt 181 gcatgatgtg cgatgtgcgG AAGGGCACCT CCACCCGATC CACATTGTTT GAAGTTATTG 241 TGAGTGCCTC GAGGACCAAG GAACCGTTGA AATTTCCAAT TTCAGGAAGG AAACCTCGAC 301 CTGTCTCCCA GTTGGTTGCA CAGCAGGTTA CTCAGCAGTT TGTGCCTCCT ACACAGTCAA 361 AGAAAGAGAA AAAAGATAAA GTAGAAAAAG AAAAAAGTGA AAAGGAAACA ACTAGCAAAA 421 AGAATAGCCA TAAGAAAACC AGGCCAAGAT TGAAAAATGT GGATCGGAGT AGTGCTCAGC 481 ATTTGGAAGT TACTGTTGGA GATCTGACAG TCATTATTAC AGACTTTAAG GAGAAAACAA 541 AGTCACCGCC TGCATCTAGT GCTGCCTCTG CAGATCAACA CAGTCAAAGC GGCTCTAGCT 601 CTGATAACAC AGAGAGAGGA ATGTCCAGGT CATCTTCACC CAGAGGAGAA GCCTCATCAT 661 TGAATGGAGA ATCTCATTAC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 721 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 781 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGACGGTATT ACTATGAGTG 841 CGCAACCACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt