Construct: ORF TRCN0000470396

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF016090.3_s317c1
Derived from:
ccsbBroadEn_14786
DNA Barcode:
GGAACTTTCTCCTGTCTCTTCTCG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PRKAR2A (5576)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000470396
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5576 PRKAR2A protein kinase cAMP-depende... NM_001321983.2 100% 100%
2 human 5576 PRKAR2A protein kinase cAMP-depende... NM_001321982.2 94.5% 94.5% 873_938del
3 human 5576 PRKAR2A protein kinase cAMP-depende... NM_004157.4 94.5% 94.5% 873_938del
4 human 5576 PRKAR2A protein kinase cAMP-depende... XM_011533942.3 94.5% 94.5% 873_938del
5 human 5576 PRKAR2A protein kinase cAMP-depende... NM_001321989.2 87.6% 87.6% 350_351ins84;789_854del
6 human 5576 PRKAR2A protein kinase cAMP-depende... XM_005265315.4 84% 77% (many diffs)
7 human 5576 PRKAR2A protein kinase cAMP-depende... XM_011533943.2 82.8% 77.9% (many diffs)
8 human 5576 PRKAR2A protein kinase cAMP-depende... XR_002959546.1 67.3% 1_237del;1110_1175del;1450_1701del
9 human 5576 PRKAR2A protein kinase cAMP-depende... XR_002959547.1 38.1% (many diffs)
10 mouse 19087 Prkar2a protein kinase, cAMP depend... NM_008924.2 81.6% 81.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1212
ORF length:
1146
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgag ccacatccag atcccgccgg ggctcacgga gctgctgcag ggctacacgg
121tggaggtgct gcgacagcag ccgcctgacc tcgtcgaatt cgcagtggag tacttcaccc
181gcctgcgcga ggcccgcgcc ccagcctcag tcctgcccgc cgccacccca cgccagagcc
241tgggccaccc cccgccagaa cccggcccgg accgtgtcgc cgacgccaaa ggggacagcg
301agtcggagga ggacgaggac ttggaagttc cagttcctag cagatttaat agacgagtat
361cagtctgtgc tgagacctat aaccctgatg aggaagagga agatacagat ccaagggtga
421ttcatcctaa aactgatgaa cagagatgca gacttcagga agcttgcaaa gatattctcc
481ttttcaaaaa tcttgatcag gaacagcttt ctcaagttct cgatgccatg tttgaaagga
541tagtcaaagc tgatgagcat gtcattgacc aaggagatga tggagacaac ttttatgtca
601tagaacgggg aacttatgac attttagtaa caaaagataa tcaaacccgc tctgttggtc
661aatatgacaa ccgtggcagt tttggagaac tagctctgat gtacaacacc ccgagagctg
721ctaccattgt tgctacctca gaaggctccc tttggggact ggaccgggtg acttttagaa
781gaatcatagt gaaaaataat gcaaagaaga ggaagatgtt tgaatcattt attGAGTCTG
841TGCCCCTCCT TAAATCACTA GAGGTGTCAG AACGAATGAA GATTGTGGAT GTAATAGGAG
901AGAAGATCTA TAAGGATGGA GAACGCATAA TCACTCAGAC TAAATCAAAC AAGGATGGTG
961GGAACCAGGA GGTCGAGATT GCCCGCTGCC ATAAGGGGCA GTACTTTGGA GAGCTTGCCC
1021TGGTCACCAA CAAACCCAGA GCTGCCTCAG CTTATGCAGT TGGAGATGTC AAATGCTTAG
1081TTATGGATGT ACAAGCATTC GAGAGGCTTC TGGGGCCCTG CATGGACATC ATGAAGAGGA
1141ACATCTCACA CTATGAGGAA CAGCTGGTGA AGATGTTTGG CTCCAGCGTG GATCTGGGCA
1201ACCTCGGGCA GTACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA
1261ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT
1321CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAGGA ACTTTCTCCT GTCTCTTCTC
1381GACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t

Download FASTA (ORF) (Full)

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