Construct: ORF TRCN0000470399

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF013525.3_s317c1
Derived from:
ccsbBroadEn_14744
DNA Barcode:
AATCGTTTATGTAGTTAGTTCAAT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PFKFB3 (5209)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000470399
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5209 PFKFB3 6-phosphofructo-2-kinase/fr... NM_004566.4 100% 100%
2 human 5209 PFKFB3 6-phosphofructo-2-kinase/fr... NM_001314063.2 98.2% 97.3% (many diffs)
3 human 5209 PFKFB3 6-phosphofructo-2-kinase/fr... XM_017016329.2 96.3% 96.3% 440_441ins57
4 human 5209 PFKFB3 6-phosphofructo-2-kinase/fr... NM_001145443.3 95.8% 94.8% (many diffs)
5 human 5209 PFKFB3 6-phosphofructo-2-kinase/fr... NM_001282630.2 95.4% 92.7% (many diffs)
6 human 5209 PFKFB3 6-phosphofructo-2-kinase/fr... NM_001323016.2 94.1% 92.1% (many diffs)
7 human 5209 PFKFB3 6-phosphofructo-2-kinase/fr... NM_001363545.2 90.4% 89.2% (many diffs)
8 human 5209 PFKFB3 6-phosphofructo-2-kinase/fr... XM_005252464.2 88.8% 88.8% 1341_1342ins174
9 human 5209 PFKFB3 6-phosphofructo-2-kinase/fr... XM_017016326.2 87.1% 85.9% (many diffs)
10 human 5209 PFKFB3 6-phosphofructo-2-kinase/fr... XM_017016327.2 86.7% 84.5% (many diffs)
11 human 5209 PFKFB3 6-phosphofructo-2-kinase/fr... XM_017016328.2 84.3% 83% (many diffs)
12 human 5209 PFKFB3 6-phosphofructo-2-kinase/fr... XM_011519493.2 82.1% 82.1% 977_978ins105;1236_1237ins174
13 human 5209 PFKFB3 6-phosphofructo-2-kinase/fr... NM_001323017.2 64.8% 64.8% 0_1ins549
14 human 5209 PFKFB3 6-phosphofructo-2-kinase/fr... XM_024448037.1 58.3% 57% (many diffs)
15 human 5209 PFKFB3 6-phosphofructo-2-kinase/fr... NR_136554.2 28.9% (many diffs)
16 mouse 170768 Pfkfb3 6-phosphofructo-2-kinase/fr... NM_133232.3 88.4% 97.6% (many diffs)
17 mouse 170768 Pfkfb3 6-phosphofructo-2-kinase/fr... NM_001177754.1 87.3% 95.5% (many diffs)
18 mouse 170768 Pfkfb3 6-phosphofructo-2-kinase/fr... NM_001177757.1 85.8% 95% (many diffs)
19 mouse 170768 Pfkfb3 6-phosphofructo-2-kinase/fr... NM_001177758.1 84.8% 92.5% (many diffs)
20 mouse 170768 Pfkfb3 6-phosphofructo-2-kinase/fr... XM_017315954.1 83.9% 93% (many diffs)
21 mouse 170768 Pfkfb3 6-phosphofructo-2-kinase/fr... NM_001177755.1 83.7% 92.5% (many diffs)
22 mouse 170768 Pfkfb3 6-phosphofructo-2-kinase/fr... NM_001177756.1 82.7% 90.5% (many diffs)
23 mouse 170768 Pfkfb3 6-phosphofructo-2-kinase/fr... NM_001177753.1 81.4% 90% (many diffs)
24 mouse 170768 Pfkfb3 6-phosphofructo-2-kinase/fr... NM_001177752.1 78% 86.7% (many diffs)
25 mouse 170768 Pfkfb3 6-phosphofructo-2-kinase/fr... XM_006497367.1 78% 85.2% (many diffs)
26 mouse 170768 Pfkfb3 6-phosphofructo-2-kinase/fr... XM_006497368.2 75.1% 83% (many diffs)
27 mouse 170768 Pfkfb3 6-phosphofructo-2-kinase/fr... XM_006497369.2 75.1% 83% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1626
ORF length:
1560
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgcc gttggaactg acgcagagcc gagtgcagaa gatctgggtg cccgtggacc
121acaggccctc gttgcccaga tcctgtgggc caaagctgac caactccccc accgtcatcg
181tcatggtggg cctccccgcc cggggcaaga cctacatctc caagaagctg actcgctacc
241tcaactggat tggcgtcccc acaaaagtgt tcaacgtcgg ggagtatcgc cgggaggctg
301tgaagcagta cagctcctac aacttcttcc gccccgacaa tgaggaagcc atgaaagtcc
361ggaagcaatg tgccttagct gccttgagag atgtcaaaag ctacctggcg aaagaagggg
421gacaaattgc ggttttcgat gccaccaata ctactagaga gaggagacac atgatccttc
481attttgccaa agaaaatgac tttaaggcgt ttttcatcga gtcggtgtgc gacgacccta
541cagttgtggc ctccaatatc atggaagtta aaatctccag cccggattac aaagactgca
601actcggcaga agccatggac gacttcatga agaggatcag ttgctatgaa gccagctacc
661agcccctcga ccccgacaaa tgcgacaggg acttgtcgct gatcaaggtg attgacgtgg
721gccggaggtt cctggtgaac cgggtgcagg accacatcca gagccgcatc gtgtactacc
781tgatgaacat ccacgtgcag ccgcgtacca tctacctgtg ccggcacggc gagaacgagc
841acaacctcca gggccgcatc gggggcgact caggcctgtc cagccggggc aagaagtttg
901ccagtgctct gagcaagttc gtggaggagc agaacctgaa ggacctgcgc gtgtggacca
961gccagctgaa gagcaccatc cagacggccg aggcgctgcg gctgccctac gagcagtgga
1021aggcgctcaa tgagatcgac gcgggcgtct gtgaggagct gacctacgag gagatcaggg
1081acacctaccc tgaggagtat gcgctgcggg agcaggacaa gtactattac cgctacccca
1141ccggggagtc ctaccaggac ctggtccagc gcttggagcc agtgatcatg gagctggagc
1201ggcaggagaa tgtgctggtc atctgccacc aggccgtccT GCGCTGCCTG CTTGCCTACT
1261TCCTGGATAA GAGTGCAGAG GAGATGCCCT ACCTGAAATG CCCTCTTCAC ACCGTCCTGA
1321AACTGACGCC TGTCGCTTAT GGCTGCCGTG TGGAATCCAT CTACCTGAAC GTGGAGTCCG
1381TCTGCACACA CCGGGAGAGG TCAGAGGATG CAAAGAAGGG ACCTAACCCG CTCATGAGAC
1441GCAATAGTGT CACCCCGCTA GCCAGCCCCG AACCCACCAA AAAGCCTCGC ATCAACAGCT
1501TTGAGGAGCA TGTGGCCTCC ACCTCGGCCG CCCTGCCCAG CTGCCTGCCC CCGGAGGTGC
1561CCACGCAGCT GCCTGGACAA AACATGAAAG GCTCCCGGAG CAGCGCTGAC TCCTCCAGGA
1621AACACTGCCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC
1681TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT
1741CTTGGCTTTA TATATCTTGT GGAAAGGACG AAATCGTTTA TGTAGTTAGT TCAATACGCG
1801TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt

Download FASTA (ORF) (Full)