Construct: ORF TRCN0000470408

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF012800.1_s317c1
Derived from:
ccsbBroadEn_11110
DNA Barcode:
GGGGCCCAAATCAATACGGCCTAT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
RPS16 (6217)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000470408
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 6217 RPS16 ribosomal protein S16 NM_001363860.2 99.5% 100% 15C>T;27T>G
2 human 6217 RPS16 ribosomal protein S16 NM_001020.6 67.3% 56.6% (many diffs)
3 human 6217 RPS16 ribosomal protein S16 NM_001321111.2 57.7% 46.3% (many diffs)
4 human 7643 ZNF90 zinc finger protein 90 XR_001753753.2 7.8% (many diffs)
5 mouse 20055 Rps16 ribosomal protein S16 NM_013647.2 60.4% 56.6% (many diffs)
6 mouse 100862433 LOC100862433 40S ribosomal protein S16 XM_003688749.3 54.2% 49.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
522
ORF length:
456
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgcc gtccaagggt ccgctgcagt cggtgcaggT CTTCGGACGC AAGAAGACAG
121CGACAGCTGT GGCGCACTGC AAACGCGGCA ATGGTCTCAT CAAGGTGAAC GGGCGGCCCC
181TGGAGATGAT TGAGCCGCGC ACGCTACAGT ACAAGCTGCT GGAGCCAGTT CTGCTTCTCG
241GCAAGGAGCG ATTTGCTGGT GTAGACATCC GTGTCCGTGT AAAGGGTGGT GGTCACGTGG
301CCCAGATTTA TGGTGAGTCC CAGGAACTGG GCGCATGGAG GAGGTGGCTC TGGGAGGGAG
361GCCTTCACAG CGCTCCTGTA CCCTTTAATT GTGTGTCTTT CTCACAGCTA TCCGTCAGTC
421CATCTCCAAA GCCCTGGTGG CCTATTACCA GAAATATGTG GATGAGGCTT CCAAGAAGGA
481GATCAAAGAC ATCCTCATCC AGTATGACCG GACCCTGCTG GTACCCAACT TTCTTGTACA
541AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT
601AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA
661AGGACGAGGG GCCCAAATCA ATACGGCCTA TACGCGTTAA GTCgacaatc aacctctgga
721ttacaaaatt tgtgaaagat t

Download FASTA (ORF) (Full)