Construct: ORF TRCN0000470408
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012800.1_s317c1
- Derived from:
- ccsbBroadEn_11110
- DNA Barcode:
- GGGGCCCAAATCAATACGGCCTAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RPS16 (6217)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470408
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6217 | RPS16 | ribosomal protein S16 | NM_001363860.2 | 99.5% | 100% | 15C>T;27T>G |
| 2 | human | 6217 | RPS16 | ribosomal protein S16 | NM_001020.6 | 67.3% | 56.6% | (many diffs) |
| 3 | human | 6217 | RPS16 | ribosomal protein S16 | NM_001321111.2 | 57.7% | 46.3% | (many diffs) |
| 4 | human | 7643 | ZNF90 | zinc finger protein 90 | XR_001753753.2 | 7.8% | (many diffs) | |
| 5 | mouse | 20055 | Rps16 | ribosomal protein S16 | NM_013647.2 | 60.4% | 56.6% | (many diffs) |
| 6 | mouse | 100862433 | LOC100862433 | 40S ribosomal protein S16 | XM_003688749.3 | 54.2% | 49.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 522
- ORF length:
- 456
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc gtccaagggt ccgctgcagt cggtgcaggT CTTCGGACGC AAGAAGACAG 121 CGACAGCTGT GGCGCACTGC AAACGCGGCA ATGGTCTCAT CAAGGTGAAC GGGCGGCCCC 181 TGGAGATGAT TGAGCCGCGC ACGCTACAGT ACAAGCTGCT GGAGCCAGTT CTGCTTCTCG 241 GCAAGGAGCG ATTTGCTGGT GTAGACATCC GTGTCCGTGT AAAGGGTGGT GGTCACGTGG 301 CCCAGATTTA TGGTGAGTCC CAGGAACTGG GCGCATGGAG GAGGTGGCTC TGGGAGGGAG 361 GCCTTCACAG CGCTCCTGTA CCCTTTAATT GTGTGTCTTT CTCACAGCTA TCCGTCAGTC 421 CATCTCCAAA GCCCTGGTGG CCTATTACCA GAAATATGTG GATGAGGCTT CCAAGAAGGA 481 GATCAAAGAC ATCCTCATCC AGTATGACCG GACCCTGCTG GTACCCAACT TTCTTGTACA 541 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 601 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 661 AGGACGAGGG GCCCAAATCA ATACGGCCTA TACGCGTTAA GTCgacaatc aacctctgga 721 ttacaaaatt tgtgaaagat t