Construct: ORF TRCN0000470431
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015264.1_s317c1
- Derived from:
- ccsbBroadEn_07476
- DNA Barcode:
- CGCCTCAAGTTATAAGACGTAATG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- UTP14C (9724)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470431
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9724 | UTP14C | UTP14C small subunit proces... | NM_021645.6 | 99.9% | 99.8% | 49G>C |
| 2 | human | 10813 | UTP14A | UTP14A small subunit proces... | NM_006649.4 | 93.7% | 89.5% | (many diffs) |
| 3 | human | 10813 | UTP14A | UTP14A small subunit proces... | NM_001166221.1 | 87.4% | 83.8% | (many diffs) |
| 4 | human | 10813 | UTP14A | UTP14A small subunit proces... | XM_017029237.1 | 74.6% | 69.4% | (many diffs) |
| 5 | human | 10813 | UTP14A | UTP14A small subunit proces... | XM_017029238.2 | 54.7% | 52.6% | (many diffs) |
| 6 | human | 440138 | ALG11 | ALG11 alpha-1,2-mannosyltra... | NR_036571.3 | 42.9% | 1_551del;600G>C;2850_5347del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2367
- ORF length:
- 2298
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaatgtgaac caggttgcag agaatctggc tttgagccac caggaacaac 121 tagtggattt gccaaaaaac taccccttga gtgaaaatga agatgagggg gacagtgatg 181 gagagagaaa gcatcaaaag cttctggaag caatcatttc ccttgatgga aagaataggc 241 ggaaattggc tgagaggtct gaggctagtc tgaaagtgtc agagttcagt gtcagttctg 301 aaggatcagg agaaaagctg ggccttgcag atctgcttga gcccgttaaa acttcatctt 361 ctttggccac tgtaaaaaag caactgaata gagtcaaatc aaagaaggtg gtggagttac 421 ctcttaacaa agaaaaaatt gaacagatcc acagagaagt agcattcagt aaaacctcac 481 aggtcctctc caaatgggac cctatcatcc tgaagaacca gcaggcagag cagctggttt 541 ttcccctggg gaaggagcag ccagccattg ctcccattga acatgcgctc agtggctgga 601 aggcaagaac tcccctggag caggaaattt ttaacctcct ccataagaac aagcagccag 661 tgacagatcc tttactgact cccatggaaa aggcctctct ccaagccatg agcctggaag 721 aggcaaagat gcaccgagca gagcttcaga gggctcgggc tctgcagtcc tactatgagg 781 ccaaggctcg aaaagagaag aaaatcaaaa gtaaaaagta tcacaaagtc gtgaagaaag 841 gaaaggccaa gaaagcctta aaagagtttg agcagctaca gaaggttaat ccaactgtgg 901 cactggaaga aatggaaaaa attgaaaatg ccagaatgat ggaaagaatg agccttaagc 961 accaaaacag tgggaaatgg gccaagtcaa aggcaattat ggccaaatat gacctggagg 1021 ctcgccaagc tatgcaggaa cagttggcca agaacaaaga actgacacag aaactccagg 1081 tagcctctga gagtgaggaa gaggagggag gcacagaagt ggaagaactc cttgtccctc 1141 atgtagcgaa tgaagtgcag atgaatgtgg acggaccgaa tccctggatg ttcaggagct 1201 gcaccagtga caccaaagag gctgcaacac aggaggaccc tgagcaagtg ccagagcttg 1261 cagctcatga ggtttctgca agtgaggcag aagaaagacc agtggcagag gaagaaattt 1321 tgttgagaga atttgaggaa aggcaatccc ttagaaaaag atctgagctc aaccaggatg 1381 ctgagccagc aagcagtcaa gaaacaaaag attctagcag ccaggaggtg ctgtccgaat 1441 tgagggcact atctcagaaa ttgaaggaaa aacatcagtc caggaagcaa aaagcaagtt 1501 cagaggggac tgttccccag gtccagagag aggaacctgc cccagaagaa gcggaacccc 1561 tattgctaca gaggtcagag agagtacaaa ctctggaaga gctagaagag ctgggaaaag 1621 aagattgttt tcaaaataag gagcttccca gacctgtgtt agaaggacag cagtcagaga 1681 ggaccccaaa taatcggcct gatgccccta aggagaagaa agagaaggag caactgatca 1741 acctacagaa cttcctgacc acacagtctc cttccgtgag gtctttggca gttcccacaa 1801 taatagagga gctggaagat gaagaggaga gagaccaaag gcagatgata aaggaagctt 1861 ttgctgggga tgatgtcatc agagatttct tgaaagagaa gagggaagct gtggaggcga 1921 gtaagccaaa ggacgtggac ctgacactac ctggctgggg cgagtggggt ggtgtgggcc 1981 taaagcccag tgccaagaaa agacgccagt ttctcattaa agcccctgag ggtcctcCAA 2041 GAAAAGATAA GAATTTGCCA AATGTGATTA TCAGTGAGAA GCGCAACATC CACGCAGCAG 2101 CTCATCAGGT ACAAGTGCTT CCATATCCAT TTACCCACCA TCGGCAATTT GAAAGGACCA 2161 TCCAGACCCC TATAGGATCC ACATGGAACA CCCAGAGGGC TTTCCAAAAG CTGACTACTC 2221 CCAAGGTCGT CACCAAGCCA GGCCATATCA TTAAGCCCAT AAAAGCAGAG GATGTGGGCT 2281 ACCAGTCTTC CTCAAGGTCA GACCTGCCTG TCATACAGAG GAATCCAAAA CGAATCACCA 2341 CACGTCACAA TAAAGAAGAA AAACTGTTGC CAACTTTCTT GTACAAAGTG GTTGATATCG 2401 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 2461 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GACGCCTCAA 2521 GTTATAAGAC GTAATGACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 2581 aagatt