Construct: ORF TRCN0000470434
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015449.1_s317c1
- Derived from:
- ccsbBroadEn_09610
- DNA Barcode:
- CTCCCATCACTATCATTTATGTTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CPNE8 (144402)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470434
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 144402 | CPNE8 | copine 8 | NM_153634.3 | 99.8% | 100% | 72G>C;789C>T |
| 2 | human | 144402 | CPNE8 | copine 8 | XR_245896.4 | 79.7% | (many diffs) | |
| 3 | human | 144402 | CPNE8 | copine 8 | XM_017018852.1 | 71.3% | 71.4% | 0_1ins483;306C>T |
| 4 | human | 144402 | CPNE8 | copine 8 | XM_011537951.3 | 69.4% | 67.9% | (many diffs) |
| 5 | human | 144402 | CPNE8 | copine 8 | XM_011537952.3 | 57.3% | 56.7% | (many diffs) |
| 6 | human | 144402 | CPNE8 | copine 8 | XR_944501.3 | 47.6% | (many diffs) | |
| 7 | mouse | 66871 | Cpne8 | copine VIII | NM_025815.2 | 87% | 96.5% | (many diffs) |
| 8 | mouse | 66871 | Cpne8 | copine VIII | XM_006521285.1 | 80.3% | 89.1% | (many diffs) |
| 9 | mouse | 66871 | Cpne8 | copine VIII | XM_006521286.3 | 69% | 75.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1761
- ORF length:
- 1692
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggacagccgc tacaacagca ctgcgggcat cggggacttg aaccagctga 121 gcgctgccat cccggccacc cgggtggagg tgtccgtgtc ctgcagaaat cttcttgaca 181 gagacacatt ttctaaatct gatccaattt gtgtcttata tgtacaagga gttggaaata 241 aagaatggag agagtttgga agaactgaag taattgataa tactttaaat cctgattttg 301 taagaaagtt tattctggac tacttttttg aagaaagaga gaatcttcgt tttgacttgt 361 atgatgttga ttcaaagagc cccaacttat ccaaacatga ctttctggga caagtgtttt 421 gtacattggg agagatcgtt ggttcacagg gaagtcgcct ggaaaaacca atagtaggaa 481 ttccagggaa gaaatgtggt acaatcatac ttacagcaga ggaattaaac tgttgcaggg 541 atgccgtttt gatgcaattt tgtgcgaaca aattggacaa gaaggacttc tttggaaaat 601 cagatccttt ccttgtattt tatcgaagta atgaagatgg cagttttaca atttgtcaca 661 agacagaagt tgtcaaaaac actctaaatc cagtatggca agcattcaag atctcagtca 721 gagcattatg taatggagac tatgacagaa caatcaaagt agaggtgtat gactgggacc 781 gagatggaag tcatgatttc attggagaat ttacaacaag ctatagggaa ctttctagag 841 ggcagtcaca attcaatgta tatgaggtgg tgaatcccaa aaagaaagga aaaaagaaaa 901 aatatactaa ttctggaaca gtaactttac tctctttctt ggtagaaaca gaagtttcat 961 tccttgacta cattaaggga gggacgcaaa tcaatttcac agtggctatt gattttacag 1021 catcaaacgg caaccctgct cagcccactt ccctccacta catgaatcct taccaactga 1081 atgcctatgg tatggcacta aaagcagtgg gagaaattgt tcaagattat gacagtgata 1141 aaatgtttcc agctctagga tttggtgcaa aactgcctcc agatggaagg atatctcacg 1201 aatttgcttt gaatgggaat cctcaaaacc cctactgtga tggcattgag ggggtcatgg 1261 aggcttatta caggagtctg aaatctgtac aactatatgg gCCCACCAAC TTTGCTCCTG 1321 TAATTAATCA TGTAGCAAGA TATGCTTCTT CTGTAAAGGA TGGCTCCCAG TATTTTGTGC 1381 TTCTGATTGT TACAGATGGT GTTATCTCAG ATATGGCCCA GACTAAGGAG TCCATAGTTA 1441 ATGCCTCAAA ACTTCCAATG TCAATAATTA TAGTAGGTGT TGGACCAGCA GAATTTGATG 1501 CAATGGTCGA ATTGGATGGA GATGATGTAA GAGTCTCCTC TAGAGGAAAA TATGCTGAAA 1561 GAGACATTGT GCAGTTTGTG CCATTCAGGG ATTATATTGA CAGAAGTGGA AACCACATAC 1621 TGAGCATGGC TAGATTGGCT AAAGATGTCC TAGCTGAGAT CCCTGAGCAG TTTCTCTCCT 1681 ATATGAGAGC CCGAGGAATC AAGCCATCAC CTGCGCCTCC CCCATACACC CCACCTACAC 1741 ATGTGTTACA GACTCAAATA TTGCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1801 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1861 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGACTCC CATCACTATC 1921 ATTTATGTTA ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt