Construct: ORF TRCN0000470447
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016404.1_s317c1
- Derived from:
- ccsbBroadEn_08272
- DNA Barcode:
- AATGCACCAAATTCCTAAGTCAGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RASL12 (51285)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470447
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51285 | RASL12 | RAS like family 12 | NM_016563.4 | 99.8% | 100% | 633C>T |
| 2 | human | 51285 | RASL12 | RAS like family 12 | NM_001307930.2 | 92.7% | 92.8% | 102_103ins57;576C>T |
| 3 | human | 51285 | RASL12 | RAS like family 12 | XM_011521660.3 | 91.5% | 87.5% | (many diffs) |
| 4 | human | 51285 | RASL12 | RAS like family 12 | XM_017022296.1 | 60.1% | 53% | (many diffs) |
| 5 | human | 51285 | RASL12 | RAS like family 12 | XM_005254434.4 | 54.3% | 54.5% | (many diffs) |
| 6 | mouse | 70784 | Rasl12 | RAS-like, family 12 | NM_001033158.2 | 88.5% | 93.6% | (many diffs) |
| 7 | mouse | 70784 | Rasl12 | RAS-like, family 12 | XM_017313587.1 | 82.4% | 82.7% | (many diffs) |
| 8 | mouse | 70784 | Rasl12 | RAS-like, family 12 | NM_001162923.1 | 54.5% | 52.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 864
- ORF length:
- 798
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc ctcggtgttt ggaaaacccc gcgcgggcag cgggcctcag agcgcgcccc 121 tcgaggtcaa cctggccatc ctggggcgcc gcggggctgg caagtctgcc ctgaccgtga 181 agtttctgac caagaggttt atcagtgaat atgaccccaa cttggaggac acctacagct 241 ccgaggagac tgtggaccac cagcctgtcc acctgagggt catggacact gcagacctgg 301 acacccccag gaactgcgag cgctacctga actgggccca tgccttcctg gtggtgtaca 361 gcgtcgacag ccgccagagc tttgatagca gcagcagcta cctggagctg cttgccTTGC 421 ACGCGAAGGA GACACAGCGC AGCATCCCTG CCCTGCTGCT GGGCAACAAG CTGGACATGG 481 CTCAGTACAG GCAAGTCACC AAGGCAGAGG GTGTGGCTTT GGCAGGCAGG TTTGGGTGCC 541 TGTTTTTCGA GGTCTCTGCC TGTCTGGACT TTGAGCACGT GCAGCATGTC TTCCACGAGG 601 CAGTGCGAGA GGCACGGCGG GAGCTGGAGA AGAGCCCCCT GACCCGGCCC CTCTTCATCT 661 CCGAGGAGAG GGCCCTGCCC CACCAGGCCC CGCTCACTGC GCGGCATGGG CTGGCCAGCT 721 GCACCTTCAA CACGCTCTCC ACCATCAACC TGAAGGAGAT GCCCACTGTG GCCCAGGCCA 781 AGCTGGTCAC CGTGAAGTCA TCCCGGGCCC AGAGCAAGCG CAAGGCGCCT ACCCTGACTC 841 TCCTGAAGGG CTTCAAGATC TTCTGCCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 901 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 961 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAA ATGCACCAAA 1021 TTCCTAAGTC AGCACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1081 att