Construct: ORF TRCN0000470514
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001392.1_s317c1
- Derived from:
- ccsbBroadEn_00685
- DNA Barcode:
- CTACAGGTGGGGCTCTCTCCAGTT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- GPX1 (2876)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470514
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2876 | GPX1 | glutathione peroxidase 1 | NM_000581.4 | 100% | 100% | |
| 2 | human | 2876 | GPX1 | glutathione peroxidase 1 | NM_001329503.2 | 78.1% | 100% | 251_269del;510_511ins118 |
| 3 | human | 2876 | GPX1 | glutathione peroxidase 1 | NM_001329455.1 | 77.3% | 24.8% | 112_113ins138 |
| 4 | human | 2876 | GPX1 | glutathione peroxidase 1 | NM_001329502.2 | 48% | 100% | (many diffs) |
| 5 | human | 2876 | GPX1 | glutathione peroxidase 1 | NM_201397.3 | 47.1% | 100% | (many diffs) |
| 6 | mouse | 14775 | Gpx1 | glutathione peroxidase 1 | NM_008160.6 | 82.7% | 85.4% | (many diffs) |
| 7 | mouse | 14775 | Gpx1 | glutathione peroxidase 1 | NM_001329527.1 | 82.3% | 85.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 210
- ORF length:
- 144
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtg tgctgctcgg ctagcggcgg cggcggcggc ggcccagtcg gtgtatgcct 121 tctcggcgcg cccgctggcc ggcggggagc ctgtgagcct gggctccctg cggggcaagg 181 tactacttat cgagaatgtg gcgtccctcT GAGGCACCAC GGTCCGGGAC TACACCCAGA 241 TGAACGAGCT GCAGCGGCGC CTCGGACCCC GGGGCCTGGT GGTGCTCGGC TTCCCGTGCA 301 ACCAGTTTGG GCATCAGGAG AACGCCAAGA ACGAAGAGAT TCTGAATTCC CTCAAGTACG 361 TCCGGCCTGG TGGTGGGTTC GAGCCCAACT TCATGCTCTT CGAGAAGTGC GAGGTGAACG 421 GTGCGGGGGC GCACCCTCTC TTCGCCTTCC TGCGGGAGGC CCTGCCAGCT CCCAGCGACG 481 ACGCCACCGC GCTTATGACC GACCCCAAGC TCATCACCTG GTCTCCGGTG TGTCGCAACG 541 ATGTTGCCTG GAACTTTGAG AAGTTCCTGG TGGGCCCTGA CGGTGTGCCC CTACGCAGGT 601 ACAGCCGCCG CTTCCAGACC ATTGACATCG AGCCTGACAT CGAAGCCCTG CTGTCTCAAG 661 GGCCCAGCTG TGCCTACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 721 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 781 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA CTACAGGTGG GGCTCTCTCC 841 AGTTACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt