Construct: ORF TRCN0000470535
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000699.1_s317c1
- Derived from:
- ccsbBroadEn_06622
- DNA Barcode:
- CTGACGTGCCCTAATTCCCTGTGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NDUFA10 (4705)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470535
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4705 | NDUFA10 | NADH:ubiquinone oxidoreduct... | NM_004544.4 | 99.8% | 100% | 105A>G;771A>G |
| 2 | human | 4705 | NDUFA10 | NADH:ubiquinone oxidoreduct... | XM_011511228.3 | 94.4% | 92% | (many diffs) |
| 3 | human | 4705 | NDUFA10 | NADH:ubiquinone oxidoreduct... | NM_001322019.1 | 89.1% | 87% | (many diffs) |
| 4 | human | 4705 | NDUFA10 | NADH:ubiquinone oxidoreduct... | NM_001322020.2 | 89.1% | 84.8% | (many diffs) |
| 5 | human | 4705 | NDUFA10 | NADH:ubiquinone oxidoreduct... | XR_001738750.2 | 62.1% | (many diffs) | |
| 6 | human | 4705 | NDUFA10 | NADH:ubiquinone oxidoreduct... | NR_136158.2 | 19.5% | (many diffs) | |
| 7 | human | 4705 | NDUFA10 | NADH:ubiquinone oxidoreduct... | NR_136155.2 | 13.4% | (many diffs) | |
| 8 | human | 4705 | NDUFA10 | NADH:ubiquinone oxidoreduct... | NR_136156.2 | 11.9% | (many diffs) | |
| 9 | human | 4705 | NDUFA10 | NADH:ubiquinone oxidoreduct... | NR_136157.2 | 11.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1131
- ORF length:
- 1065
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cttgcggctc ctgaagctgg cagcgacgtc cgcgtccgcc cgggtcgtgg 121 cggcgggcgc ccagcgcgtg agaggaattc atagcagtgt gcagtgcaag ctgcgctatg 181 gaatgtggca tttcctactt ggggataaag caagcaaaag actgacagaa cgcagcagag 241 tgataactgt agatggcaat atatgtactg gaaaaggcaa acttgcaaaa gaaatagcag 301 agaaactagg cttcaagcac tttcctgaag cggggattca ttatccagac agtaccacag 361 gagatgggaa gcccctcgcc accgactata atggcaactg tagtttggag aaattttacg 421 atgatccgag aagcaatgat ggcaacagtt accgcctgca gtcctggttg tacagcagtc 481 gcctgctgca gtactcagat gccttggagc acttgctgac cacaggacaa ggtgttgtgt 541 tggagcgctc catcttcagt gactttgtgt tcctGGAGGC GATGTACAAC CAGGGATTCA 601 TCCGAAAGCA GTGTGTGGAC CACTACAACG AGGTGAAGAG CGTCACCATC TGCGATTACC 661 TGCCCCCCCA CCTGGTGATT TACATCGATG TGCCCGTTCC AGAGGTCCAG AGGCGGATTC 721 AGAAGAAAGG AGATCCACAT GAAATGAAGA TCACCTCTGC CTATCTACAG GACATTGAGA 781 ATGCCTATAA GAAAACCTTT CTCCCTGAGA TGAGTGAAAA ATGTGAGGTT TTACAGTATT 841 CTGCAAGGGA AGCTCAAGAT TCAAAAAAGG TGGTAGAGGA CATTGAATAC CTGAAGTTCG 901 ATAAAGGGCC GTGGCTCAAG CAGGACAATC GCACTTTATA CCACCTGCGA TTACTGGTTC 961 AGGATAAGTT TGAGGTGCTG AATTACACAA GCATTCCTAT CTTTCTCCCG GAAGTCACCA 1021 TTGGAGCTCA TCAGACTGAC CGTGTCTTAC ATCAGTTCAG AGAGCTGCCG GGCCGCAAGT 1081 ACAGCCCTGG GTACAACACC GAGGTGGGAG ACAAGTGGAT CTGGCTGAAG TGCCCAACTT 1141 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1201 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1261 CTTGTGGAAA GGACGACTGA CGTGCCCTAA TTCCCTGTGC ACGCGTTAAG TCgacaatca 1321 acctctggat tacaaaattt gtgaaagatt