Construct: ORF TRCN0000470550
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017401.1_s317c1
- Derived from:
- ccsbBroadEn_12745
- DNA Barcode:
- AGCGCGAAGTGTATTCCTACTCGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CCM2 (83605)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470550
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 83605 | CCM2 | CCM2 scaffold protein | XM_017012671.1 | 21.7% | 18.8% | (many diffs) |
| 2 | human | 83605 | CCM2 | CCM2 scaffold protein | NM_001167935.1 | 20% | 19.2% | (many diffs) |
| 3 | human | 83605 | CCM2 | CCM2 scaffold protein | XM_006715786.3 | 18.5% | 16% | (many diffs) |
| 4 | human | 83605 | CCM2 | CCM2 scaffold protein | NM_031443.3 | 15.9% | 15.3% | (many diffs) |
| 5 | human | 83605 | CCM2 | CCM2 scaffold protein | NM_001029835.2 | 14.8% | 12.9% | (many diffs) |
| 6 | human | 83605 | CCM2 | CCM2 scaffold protein | NM_001363458.2 | 14.5% | 14% | (many diffs) |
| 7 | human | 83605 | CCM2 | CCM2 scaffold protein | XM_011515561.2 | 13.6% | 11.9% | (many diffs) |
| 8 | human | 83605 | CCM2 | CCM2 scaffold protein | XR_428088.3 | 11.7% | (many diffs) | |
| 9 | human | 83605 | CCM2 | CCM2 scaffold protein | NR_030770.2 | 11.1% | (many diffs) | |
| 10 | mouse | 216527 | Ccm2 | cerebral cavernous malforma... | NM_146014.3 | 15% | 14.7% | (many diffs) |
| 11 | mouse | 216527 | Ccm2 | cerebral cavernous malforma... | XM_006514622.1 | 13.7% | 13.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 194
- ORF end:
- 407
- ORF length:
- 213
- Sequence:
-
1 CACACTGAGT GGGTGGAGAC TGAAGTTAGG CCAGCTTGGC ACTTGATGTA ATTCTCCTTG 61 GAATTTGCCC TTTTTGAGTT TGGATCTTGG TTCATTCTCA AGCCTCAGAC AGTGGTTCAA 121 AGTTTTTTTC TTCCATTTCA GGTGTCGTGA GGCTAGCATC GATTGATCAA CAAGTTTGTA 181 CAAAAAAGTT GGCATGGAAG AGGAGGGCAA GAAGGGCAAG AAGCCTGGAA TTGTCTCGCC 241 ATTTAAACGA GTATTCCTAA AAGGTGAAAA GAGTAGAGAT AAGAAAGCCC ATGAGAAGGT 301 GACAGAGAGG CGCCCTCTGC ACACTGTGGT GTTGTCATTG CCTGAGCGCG TCGAGCCAGA 361 CAGACTGCTG AGCGACTATA TTGAGAAGGA GGTAAAGGGC TCCCATTACC CAACTTTCTT 421 GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC 481 GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG 541 TGGAAAGGAC GAAGCGCGAA GTGTATTCCT ACTCGGACGC GTTAAGTCga caatcaacct 601 ctggattaca aaatttgtga aagatt