Construct: ORF TRCN0000470566
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007017.1_s317c1
- Derived from:
- ccsbBroadEn_04024
- DNA Barcode:
- CATCAGCTGAAGCTCCTCATATCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DDX50 (79009)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470566
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79009 | DDX50 | DExD-box helicase 50 | NM_024045.2 | 99.9% | 100% | 1617C>T |
| 2 | human | 79009 | DDX50 | DExD-box helicase 50 | XM_017016626.1 | 99.9% | 100% | 1617C>T |
| 3 | human | 79009 | DDX50 | DExD-box helicase 50 | XM_005270148.2 | 91.1% | 91.1% | 0_1ins195;1422C>T |
| 4 | human | 79009 | DDX50 | DExD-box helicase 50 | XM_011540144.2 | 91.1% | 91.1% | 0_1ins195;1422C>T |
| 5 | human | 79009 | DDX50 | DExD-box helicase 50 | XM_017016627.1 | 91.1% | 91.1% | 0_1ins195;1422C>T |
| 6 | human | 79009 | DDX50 | DExD-box helicase 50 | XM_017016628.1 | 91.1% | 91.1% | 0_1ins195;1422C>T |
| 7 | human | 79009 | DDX50 | DExD-box helicase 50 | XM_024448162.1 | 91.1% | 91.1% | 0_1ins195;1422C>T |
| 8 | human | 79009 | DDX50 | DExD-box helicase 50 | XM_017016630.1 | 60% | 60.1% | 0_1ins882;735C>T |
| 9 | mouse | 94213 | Ddx50 | DEAD (Asp-Glu-Ala-Asp) box ... | NM_053183.2 | 92.1% | 95.6% | (many diffs) |
| 10 | mouse | 94213 | Ddx50 | DEAD (Asp-Glu-Ala-Asp) box ... | XM_006514345.3 | 85.4% | 88.3% | (many diffs) |
| 11 | mouse | 94213 | Ddx50 | DEAD (Asp-Glu-Ala-Asp) box ... | XM_006514346.3 | 84.1% | 87.9% | (many diffs) |
| 12 | mouse | 94213 | Ddx50 | DEAD (Asp-Glu-Ala-Asp) box ... | XM_011243608.2 | 56.3% | 59% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2277
- ORF length:
- 2211
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc tgggaaactc ctctgggggg acattatgga gctggaagca cccttggagg 121 agtccgagag ccagaagaag gagaggcaaa agagtgacag aaggaagtca aggcaccatt 181 atgactcgga tgagaaatca gaaacaagag aaaatggtgt tacagatgac ctggatgctc 241 ccaaggccaa aaaatctaaa atgaaagaga agctaaatgg agacactgaa gaaggattta 301 atagactttc agatgaattc tccaaatctc ataagtcaag aagaaaagat ctaccaaatg 361 gagatataga tgaatatgaa aaaaaatcaa agcgagtatc atctttagat acttctactc 421 ataaatcaag tgataataaa ctagaggaga ccttaacacg tgaacagaaa gaaggagcct 481 tctccaattt tcctatttct gaagagacta taaagcttct gaaaggtcga ggggtaacat 541 atctctttcc tattcaagtt aagacctttg gtcctgtata tgaaggaaaa gatttaatag 601 ctcaagcacg gacaggaaca ggaaagacat tctcttttgc catcccctta attgaaagac 661 tccaaagaaa tcaagaaaca attaaaaaaa gccgctcacc aaaggtactt gttttggctc 721 caacaaggga actggcaaac caagtagcca aagacttcaa agatataact aggaaactca 781 gcgtggcgtg tttttatggt ggaacatcat atcaaagcca aattaatcat attcgaaatg 841 gtattgacat cttggttgga acacctggtc gtatcaaaga ccatctgcag agtggccgat 901 tggatctttc taaactgcga catgttgtgc ttgatgaagt ggatcagatg ttagatttag 961 gtttcgctga acaagttgaa gatattattc atgaatccta caaaactgat tctgaagaca 1021 atcctcagac tttacttttt tctgcaactt gcccacagtg ggtatacaaa gttgcaaaaa 1081 aatacatgaa atccagatat gaacaggttg accttgttgg aaaaatgact caaaaggctg 1141 caactactgt ggaacatttg gccatccagt gtcattggtc tcagaggcca gcagttattg 1201 gagatgtcct tcaagtctac agtgggtctg aagggagggc tattattttc tgtgagacca 1261 agaagaatgt aactgaaatg gccatgaatc cacacataaa acagaatgcc cagtgtttac 1321 atggggacat tgcacagtca caaagagaaa ttacactaaa aggcttcaga gaaggtagtt 1381 ttaaagtttt ggtggcaacc aatgtggctg cccgtggttt ggacattcct gaagttgacc 1441 tggtgattca aagttctcct cctcaggatg ttgagtccta tatccatcgc tctggacgca 1501 caggtagagc tggacggaca gggatttgta tatgttttta tcaaccaaga gaaagaggtc 1561 aactaagata tgtggaacaa aaagcaggaa ttacttttaa acgtgtaggt gttccttcta 1621 caatggattt agttaaatct aaaagcatgg atgccatcag gtctctggct tccgtttctt 1681 atgctgctgt tgattttttc cgaccatcag ctcagagact gatagaagag aaaggtgcag 1741 tggatgcatt ggctgcagct ttagcccaca tttctggtgc atcaagcttt gaaccacgat 1801 ctttgatcac ctctgataag ggGTTTGTGA CCATGACTCT GGAAAGCCTA GAGGAAATAC 1861 AGGATGTCAG CTGTGCTTGG AAAGAACTTA ACAGAAAGCT GAGTAGTAAT GCAGTGTCTC 1921 AGATTACCAG AATGTGCCTC CTGAAAGGAA ATATGGGTGT TTGCTTTGAT GTTCCTACAA 1981 CTGAGTCAGA AAGGTTACAG GCAGAGTGGC ATGATTCCGA CTGGATACTC TCAGTGCCAG 2041 CCAAATTACC TGAAATTGAA GAATATTATG ATGGAAACAC ATCTTCTAAT TCCAGACAGA 2101 GGAGTGGCTG GTCAAGTGGT CGATCAGGCC GGTCAGGCCG GTCAGGTGGT CGATCTGGCG 2161 GCCGGTCAGG TAGACAGAGT CGACAAGGAA GTCGCTCAGG AAGTCGACAA GATGGTAGAA 2221 GACGAAGTGG GAATAGAAAT CGATCAAGAA GTGGGGGCCA CAAACGGAGT TTTGACTGCC 2281 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 2341 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 2401 ATATATCTTG TGGAAAGGAC GACATCAGCT GAAGCTCCTC ATATCGACGC GTTAAGTCga 2461 caatcaacct ctggattaca aaatttgtga aagatt