Transcript: Human XM_024448162.1

PREDICTED: Homo sapiens DExD-box helicase 50 (DDX50), transcript variant X6, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
DDX50 (79009)
Length:
5375
CDS:
3140..5158

Additional Resources:

NCBI RefSeq record:
XM_024448162.1
NBCI Gene record:
DDX50 (79009)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_024448162.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000050991 GCCAAAGACTTCAAAGATATA pLKO.1 3626 CDS 100% 13.200 9.240 N DDX50 n/a
2 TRCN0000289417 GCCAAAGACTTCAAAGATATA pLKO_005 3626 CDS 100% 13.200 9.240 N DDX50 n/a
3 TRCN0000050989 CGGATGAGAAATCAGAAACAA pLKO.1 3066 5UTR 100% 5.625 3.938 N DDX50 n/a
4 TRCN0000289415 CGGATGAGAAATCAGAAACAA pLKO_005 3066 5UTR 100% 5.625 3.938 N DDX50 n/a
5 TRCN0000050990 CCAAATCTCATAAGTCAAGAA pLKO.1 3201 CDS 100% 4.950 3.465 N DDX50 n/a
6 TRCN0000289482 CCAAATCTCATAAGTCAAGAA pLKO_005 3201 CDS 100% 4.950 3.465 N DDX50 n/a
7 TRCN0000050992 CGACATGTTGTGCTTGATGAA pLKO.1 3797 CDS 100% 4.950 3.465 N DDX50 n/a
8 TRCN0000289414 CGACATGTTGTGCTTGATGAA pLKO_005 3797 CDS 100% 4.950 3.465 N DDX50 n/a
9 TRCN0000050988 CCCTTAATTGAAAGACTCCAA pLKO.1 3524 CDS 100% 2.640 1.848 N DDX50 n/a
10 TRCN0000104019 CCAGCCAAATTACCTGAAATT pLKO.1 4916 CDS 100% 13.200 7.920 N Ddx50 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_024448162.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_04024 pDONR223 100% 91.1% 91.1% None 0_1ins195;1422C>T n/a
2 ccsbBroad304_04024 pLX_304 0% 91.1% 91.1% V5 0_1ins195;1422C>T n/a
3 TRCN0000470566 CATCAGCTGAAGCTCCTCATATCG pLX_317 20.8% 91.1% 91.1% V5 0_1ins195;1422C>T n/a
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