Construct: ORF TRCN0000470575
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013139.1_s317c1
- Derived from:
- ccsbBroadEn_06073
- DNA Barcode:
- CTAAAATCTCGCTGGTGGCATAGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CYP3A7 (1551)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470575
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1551 | CYP3A7 | cytochrome P450 family 3 su... | NM_000765.5 | 99.9% | 100% | 945C>T |
| 2 | human | 1576 | CYP3A4 | cytochrome P450 family 3 su... | NM_017460.6 | 94.3% | 88.4% | (many diffs) |
| 3 | human | 1576 | CYP3A4 | cytochrome P450 family 3 su... | NM_001202855.3 | 94.2% | 88.2% | (many diffs) |
| 4 | human | 100861540 | CYP3A7-CYP3A51P | CYP3A7-CYP3A51P readthrough | NM_001256497.3 | 93.8% | 93.2% | (many diffs) |
| 5 | human | 1577 | CYP3A5 | cytochrome P450 family 3 su... | NM_000777.5 | 88.4% | 81.7% | (many diffs) |
| 6 | human | 1577 | CYP3A5 | cytochrome P450 family 3 su... | NM_001291830.2 | 85.7% | 77.9% | (many diffs) |
| 7 | human | 1577 | CYP3A5 | cytochrome P450 family 3 su... | NM_001291829.2 | 68.8% | 63.2% | (many diffs) |
| 8 | human | 1577 | CYP3A5 | cytochrome P450 family 3 su... | NR_033807.3 | 40.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1575
- ORF length:
- 1509
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga tctcatccca aacttggccg tggaaacctg gcttctcctg gctgtcagcc 121 tgatactcct ctatctatat ggaacccgta cacatggact ttttaagaag cttggaattc 181 cagggcccac acctctgcct tttttgggaa atgctttgtc cttccgtaag ggctattgga 241 cgtttgacat ggaatgttat aaaaagtata gaaaagtctg gggtatttat gactgtcaac 301 agcctatgct ggctatcaca gatcccgaca tgatcaaaac agtgctagtg aaagaatgtt 361 attctgtctt cacaaaccgg aggcctttcg ggccagtggg atttatgaaa aatgccatct 421 ctatagctga ggatgaagaa tggaagagaa tacgatcatt gctgtctcca acattcacca 481 gcggaaaact caaggagatg gtccctatca ttgcccagta tggagatgtg ttggtgagaa 541 atctgaggcg ggaagcagag acaggcaagc ctgtcacctt gaaacacgtc tttggggcct 601 acagcatgga tgtgatcact agcacatcat ttggagtgag catcgactct ctcaacaatc 661 cacaagaccc ctttgtggaa aacaccaaga agcttttaag atttaatcca ttagatccat 721 tcgttctctc aataaaagtc tttccattcc ttaccccaat tcttgaagca ttaaatatca 781 ctgtgtttcc aagaaaagtt ataagttttc taacaaaatc tgtaaaacag ataaaagaag 841 gtcgcctcaa agagacacaa aagcaccgag tggatttcct tcagctgatg attgactctc 901 agaattcaaa agactctgag acccacaaag ctctgtctga tctggagctc atggcccaat 961 caattatctt tatttttgct ggctatgaaa ccacgagcag tgttctctct ttcattatat 1021 atgaactggc cactcaccct gatgtccagc agaaagtgca gaaggaaatt gatacagttt 1081 tacccaataa ggcaccaccc acctatgata ctgtgctaca gttGGAGTAT CTTGACATGG 1141 TGGTGAATGA AACACTCAGA TTATTCCCAG TTGCTATGAG ACTTGAGAGG GTCTGCAAAA 1201 AAGATGTTGA AATCAATGGG ATGTTTATTC CCAAAGGGGT GGTGGTGATG ATTCCAAGCT 1261 ATGTTCTTCA TCATGACCCA AAGTACTGGA CAGAGCCTGA GAAGTTCCTC CCTGAAAGGT 1321 TCAGTAAAAA GAACAAGGAC AACATAGATC CTTACATATA CACACCCTTT GGAAGTGGAC 1381 CCAGAAACTG CATTGGCATG AGGTTTGCTC TCGTGAACAT GAAACTTGCT CTAGTCAGAG 1441 TCCTTCAGAA CTTCTCCTTC AAACCTTGTA AAGAAACACA GATCCCCCTG AAATTACGCT 1501 TTGGAGGACT TCTTCTAACA GAAAAACCCA TTGTTCTAAA GGCTGAGTCA AGGGATGAGA 1561 CCGTAAGTGG AGCCTGCCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1621 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1681 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA CTAAAATCTC GCTGGTGGCA 1741 TAGCACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt