Transcript: Human NM_001202855.3

Homo sapiens cytochrome P450 family 3 subfamily A member 4 (CYP3A4), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-09-29
Taxon:
Homo sapiens (human)
Gene:
CYP3A4 (1576)
Length:
2778
CDS:
104..1612

Additional Resources:

NCBI RefSeq record:
NM_001202855.3
NBCI Gene record:
CYP3A4 (1576)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001202855.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000064224 CATCCCAATTCTTGAAGTATT pLKO.1 787 CDS 100% 13.200 9.240 N CYP3A4 n/a
2 TRCN0000436334 CTGATAAGAGAATCAACATTT pLKO_005 1909 3UTR 100% 13.200 9.240 N CYP3A4 n/a
3 TRCN0000417816 TCTGCTTCTCACGGGACTATT pLKO_005 1848 3UTR 100% 13.200 9.240 N CYP3A4 n/a
4 TRCN0000064223 GTTTCCAAGAGAAGTTACAAA pLKO.1 820 CDS 100% 5.625 3.938 N CYP3A4 n/a
5 TRCN0000419180 AGTCGCCTCGAAGATACACAA pLKO_005 875 CDS 100% 4.950 3.465 N CYP3A4 n/a
6 TRCN0000064225 CCCTGAAAGATTCAGCAAGAA pLKO.1 1345 CDS 100% 4.950 3.465 N CYP3A4 n/a
7 TRCN0000064227 GAACCCATTCACATGGACTTT pLKO.1 180 CDS 100% 4.950 2.970 N CYP3A4 n/a
8 TRCN0000064020 CCTTACATATACACACCCTTT pLKO.1 1385 CDS 100% 4.050 2.025 Y CYP3A7 n/a
9 TRCN0000064226 CCCACCTATGATACTGTGCTA pLKO.1 1133 CDS 100% 2.640 1.320 Y CYP3A4 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001202855.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00411 pDONR223 100% 99.8% 99.6% None 670_671insCAG n/a
2 ccsbBroad304_00411 pLX_304 0% 99.8% 99.6% V5 670_671insCAG n/a
3 TRCN0000477588 ATCTATTATTTATGACGGCGACCT pLX_317 35.4% 99.8% 99.6% V5 670_671insCAG n/a
4 ccsbBroadEn_06073 pDONR223 100% 94.2% 88.2% None (many diffs) n/a
5 ccsbBroad304_06073 pLX_304 0% 94.2% 88.2% V5 (many diffs) n/a
6 TRCN0000470575 CTAAAATCTCGCTGGTGGCATAGC pLX_317 30.2% 94.2% 88.2% V5 (many diffs) n/a
7 ccsbBroadEn_00412 pDONR223 100% 89.2% 84.2% None (many diffs) n/a
8 ccsbBroad304_00412 pLX_304 0% 89.2% 84.2% V5 (many diffs) n/a
9 TRCN0000468230 ATCAACTGGCCTGCGCCACCCCTT pLX_317 31.3% 89.2% 84.2% V5 (many diffs) n/a
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