Construct: ORF TRCN0000470580
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001584.1_s317c1
- Derived from:
- ccsbBroadEn_05392
- DNA Barcode:
- ATAGAAGACTGCCCTACCCTTCTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EOGT (285203)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470580
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 285203 | EOGT | EGF domain specific O-linke... | NM_173654.3 | 99.9% | 100% | 465A>G |
| 2 | human | 285203 | EOGT | EGF domain specific O-linke... | NM_001278689.2 | 83.9% | 84% | 465A>G;832_1083del |
| 3 | human | 285203 | EOGT | EGF domain specific O-linke... | XM_005264743.2 | 83.9% | 84% | 465A>G;832_1083del |
| 4 | human | 285203 | EOGT | EGF domain specific O-linke... | XM_017006205.1 | 83.9% | 84% | 465A>G;832_1083del |
| 5 | human | 285203 | EOGT | EGF domain specific O-linke... | XM_017006206.1 | 83.9% | 84% | 465A>G;832_1083del |
| 6 | human | 285203 | EOGT | EGF domain specific O-linke... | XM_011533603.1 | 81.3% | 81.4% | 465A>G;832_1134del |
| 7 | human | 285203 | EOGT | EGF domain specific O-linke... | XM_017006204.1 | 81.3% | 81.4% | 465A>G;832_1134del |
| 8 | human | 285203 | EOGT | EGF domain specific O-linke... | XM_011533599.1 | 81.2% | 81.2% | 465A>G;832_1137del |
| 9 | human | 285203 | EOGT | EGF domain specific O-linke... | XM_011533600.1 | 81.2% | 81.2% | 465A>G;832_1137del |
| 10 | human | 285203 | EOGT | EGF domain specific O-linke... | XM_011533602.1 | 81.2% | 81.2% | 465A>G;832_1137del |
| 11 | human | 285203 | EOGT | EGF domain specific O-linke... | XM_017006208.1 | 64.8% | 64.1% | (many diffs) |
| 12 | human | 285203 | EOGT | EGF domain specific O-linke... | XM_017006207.1 | 56.3% | 36.3% | (many diffs) |
| 13 | human | 285203 | EOGT | EGF domain specific O-linke... | XM_017006209.1 | 50.1% | 38.8% | (many diffs) |
| 14 | human | 285203 | EOGT | EGF domain specific O-linke... | NR_103826.2 | 28.8% | (many diffs) | |
| 15 | mouse | 101351 | Eogt | EGF domain-specific O-linke... | NM_175313.4 | 71.2% | 72.6% | (many diffs) |
| 16 | mouse | 101351 | Eogt | EGF domain-specific O-linke... | XM_006505258.3 | 71.2% | 72.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1395
- ORF length:
- 1329
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt aatgttgttt gtctttggag tcttacttca tgaagtctca ctgagtggtc 121 agaatgaagc tcctcctaat actcacagca ttccaggcga acctctgtat aactatgcca 181 gcatccgctt gccagaggag cacattccct tctttttgca caacaatagg catattgcca 241 ctgtctgtag gaaagactct ctttgtccat ataagaaaca cctagagaag ctaaagtact 301 gctggggtta tgagaaatcc tgcaaaccag agttcaggtt tggttaccca gtttgcagct 361 atgtcgacat gggatggacg gacactcttg agtcagctga ggacatattt tggaaacaag 421 ctgactttgg atatgccaga gagaggctgg aggagatgca tgtgctctgt cagcctaagg 481 aaacgagtga ctcaagtctg gtgtgttccc gttatcttca gtactgcagg gcaaccaatc 541 tctatcttga tttaagaaac atcaagagaa atcatgacag atttaaggag gactttttcc 601 agagtggtga aattggaggg cactgtaaac ttgacatccg tacattgacg tctgaaggtc 661 agcgcaaaag ccctctgcag tcatggtttg ctgagctaca aagctatact cagctcaact 721 tcagacctat agaagatgct aaatgtgaca ttgtcattga aaaaccaaca tatttcatga 781 aattagatgc aggtgttaac atgtatcacc acttctgtga tttcatcaat ctttatatta 841 ctcagcacgt taataactca ttcagtactg acgtgtacat cgtgatgtgg gacaccgatg 901 gaaaaattcg agtcaccatT CTTGCACGGA GCACAGAATA CCGGAAAATC CTTAACCAAA 961 ATGAGCTTGT AAATGCACTG AAAACAGTAT CTACATTTGA AGTCCAGATT GTTGATTACA 1021 AGTATAGAGA ACTTGGGTTT TTAGATCAAC TAAGGATCAC ACACAACACG GACATATTTA 1081 TTGGAATGCA TGGAGCTGGT CTGACCCATT TACTTTTCCT TCCAGACTGG GCTGCTGTAT 1141 TTGAACTGTA CAACTGTGAA GATGAACGCT GTTACTTAGA CTTGGCCAGG CTGAGAGGCG 1201 TTCACTACAT CACTTGGCGA CGGCAGAACA AAGTCTTTCC TCAGGATAAG GGCCACCATC 1261 CAACCCTGGG GGAGCACCCG AAGTTCACCA ACTACTCTTT CGATGTAGAA GAATTTATGT 1321 ATCTTGTCCT TCAGGCTGCA GACCACGTAT TGCAACACCC AAAGTGGCCA TTTAAGAAGA 1381 AACATGATGA GCTATACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1441 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1501 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA ATAGAAGACT GCCCTACCCT 1561 TCTTACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt