Construct: ORF TRCN0000470664
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011551.1_s317c1
- Derived from:
- ccsbBroadEn_02323
- DNA Barcode:
- TGTACCGAAAAATACCTCCTATGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PDIA6 (10130)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470664
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10130 | PDIA6 | protein disulfide isomerase... | NM_005742.4 | 100% | 100% | |
| 2 | human | 10130 | PDIA6 | protein disulfide isomerase... | NM_001282707.1 | 99.1% | 98.8% | (many diffs) |
| 3 | human | 10130 | PDIA6 | protein disulfide isomerase... | NM_001282706.1 | 97.7% | 97.7% | (many diffs) |
| 4 | human | 10130 | PDIA6 | protein disulfide isomerase... | XM_011510308.2 | 91.3% | 88.4% | 1155_1156ins97;1206_1207insTAGGGAAAGATGAGTTG |
| 5 | human | 10130 | PDIA6 | protein disulfide isomerase... | NM_001282705.2 | 89.1% | 89.1% | (many diffs) |
| 6 | human | 10130 | PDIA6 | protein disulfide isomerase... | NM_001282704.1 | 88.4% | 88.4% | (many diffs) |
| 7 | mouse | 71853 | Pdia6 | protein disulfide isomerase... | NM_027959.3 | 86% | 94.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1386
- ORF length:
- 1320
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tctcctggtg ctcggtctgg tgagctgtac cttctttctg gcagtgaatg 121 gtctgtattc ctctagtgat gatgtgatcg aattaactcc atcgaatttc aaccgagaag 181 ttattcagag tgatagtttg tggcttgtag aattctatgc tccatggtgt ggtcactgtc 241 aaagattaac accagaatgg aagaaagcag caactgcatt aaaagatgtt gtcaaagttg 301 gtgcagttga tgcagataag catcattccc taggaggtca gtatggtgtt cagggatttc 361 ctaccattaa gatttttgga tccaacaaaa acagaccaga agattaccaa ggtggcagaa 421 ctggtgaagc cattgtagat gctgcgctga gtgctctgcg ccagctcgtg aaggatcgcc 481 tcgggggacg gagcggagga tacagttctg gaaaacaagg cagaagtgat agttcaagta 541 agaaggatgt gattgagctg acagacgaca gctttgataa gaatgttctg gacagtgaag 601 atgtttggat ggttgagttc tatgctcctt ggtgtggaca ctgcaaaaac ctagagccag 661 agtgggctgc cgcagcttca gaagtaaaag agcagacgaa aggaaaagtg aaactggcag 721 ctgtggatgc tacagtcaat caggttctgg cctcccgata cgggattaga ggatttccta 781 caatcaagat atttcagaaa ggcgagtctc ctgtggatta tgacggtggg cggacaagat 841 ccgacatcgt gtcccgggcc cttgatttgt tttctgataa cgccccacct cctGAGCTGC 901 TTGAGATTAT CAACGAGGAC ATTGCCAAGA GGACGTGTGA GGAGCACCAG CTCTGTGTTG 961 TGGCTGTGCT GCCCCATATC CTTGATACTG GAGCTGCAGG CAGAAATTCT TATCTGGAAG 1021 TTCTTCTGAA GTTGGCAGAC AAATACAAAA AGAAAATGTG GGGGTGGCTG TGGACAGAAG 1081 CTGGAGCCCA GTCTGAACTT GAGACCGCGT TGGGGATTGG AGGGTTTGGG TACCCCGCCA 1141 TGGCCGCCAT CAATGCACGC AAGATGAAAT TTGCTCTGCT AAAAGGCTCC TTCAGTGAGC 1201 AAGGCATCAA CGAGTTTCTC AGGGAGCTCT CTTTTGGGCG TGGCTCCACG GCACCTGTAG 1261 GAGGCGGGGC TTTCCCTACC ATCGTTGAGA GAGAGCCTTG GGACGGCAGG GATGGCGAGC 1321 TTCCCGTGGA GGATGACATT GACCTCAGTG ATGTGGAGCT TGATGACTTA GGGAAAGATG 1381 AGTTGTGCCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1441 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1501 CTTGGCTTTA TATATCTTGT GGAAAGGACG ATGTACCGAA AAATACCTCC TATGGACGCG 1561 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt