Construct: ORF TRCN0000470668
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002313.1_s317c1
- Derived from:
- ccsbBroadEn_15772
- DNA Barcode:
- CCACGCCACATGCTCTAAGAGTAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SULT4A1 (25830)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470668
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 25830 | SULT4A1 | sulfotransferase family 4A ... | NM_014351.4 | 99.5% | 99.2% | 163_166delAAGTinsC |
| 2 | human | 25830 | SULT4A1 | sulfotransferase family 4A ... | XM_024452212.1 | 72.4% | 72.4% | 0_1ins234 |
| 3 | human | 25830 | SULT4A1 | sulfotransferase family 4A ... | XM_011530121.1 | 60.1% | 60% | (many diffs) |
| 4 | human | 25830 | SULT4A1 | sulfotransferase family 4A ... | XM_011530120.3 | 59.4% | 59.1% | 163_166delAAGTinsC;509delA;513_513delAins341 |
| 5 | human | 25830 | SULT4A1 | sulfotransferase family 4A ... | XR_937839.3 | 58.7% | (many diffs) | |
| 6 | human | 25830 | SULT4A1 | sulfotransferase family 4A ... | XR_937838.2 | 33.5% | (many diffs) | |
| 7 | human | 25830 | SULT4A1 | sulfotransferase family 4A ... | XR_001755207.1 | 32.9% | (many diffs) | |
| 8 | mouse | 29859 | Sult4a1 | sulfotransferase family 4A,... | NM_013873.3 | 91% | 97.1% | (many diffs) |
| 9 | mouse | 29859 | Sult4a1 | sulfotransferase family 4A,... | XM_006521065.3 | 80.7% | 80.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 915
- ORF length:
- 849
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggagagcgag gccgagaccc ccagcacccc gggggagttc gagagcaagt 121 acttcgagtt ccatggcgtg cggctgccgc ccttctgccg cgggaagatg gaggagatcg 181 ccaacttccc ggtgcggccc agcgacgtgt ggatcgtcac ctaccccccc ggcaccagct 241 tgctgcagga ggtggtctac ttggtgagcc agggcgctga ccccgatgag atcggcttga 301 tgaacatcga cgagcagctc ccggtcctgg agtacccaca gccgggcctg gacatcatca 361 aggaactgac ctctccccgc ctcatcaaga gccacctgcc ctaccgcttt ctgccctctg 421 acctccacaa tggagactcc aaggtcatct atatggctcg caaccccaag gatctggtgg 481 tgtcttatta tcagttccac cgctctctgc ggaccatgag ctaccgaggc acctttcaag 541 aattctgccg gaggtttatg aatgataagc tgggctacgg ctcctggttt gagcacgtgc 601 aggagttctg ggagcaccgc atggactcga acgtgctttt tctcaagtat gaagacatgc 661 atcgggacct ggtgacgatg gtggagcagc tggccagatt cctgggggtg tcctgtgaca 721 aggcccagct ggaagccctg acggagcact gccaCCAGCT GGTGGACCAG TGCTGCAACG 781 CTGAGGCCCT GCCCGTGGGC CGGGGAAGAG TTGGGCTGTG GAAGGACATC TTCACCGTCT 841 CCATGAATGA GAAGTTTGAC TTGGTGTATA AACAGAAGAT GGGAAAGTGT GACCTCACGT 901 TTGACTTTTA TTTATACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 961 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1021 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA CCACGCCACA TGCTCTAAGA 1081 GTATACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt