Construct: ORF TRCN0000470686
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012846.1_s317c1
- Derived from:
- ccsbBroadEn_09918
- DNA Barcode:
- TATCTAGGGATAAAATCTACCATT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HEATR9 (256957)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470686
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 256957 | HEATR9 | HEAT repeat containing 9 | NM_152781.4 | 99.9% | 99.8% | 8A>G |
| 2 | human | 256957 | HEATR9 | HEAT repeat containing 9 | XM_005278233.2 | 94% | 94% | 0_1ins102 |
| 3 | human | 256957 | HEATR9 | HEAT repeat containing 9 | XM_011524586.2 | 94% | 94% | 0_1ins102 |
| 4 | human | 256957 | HEATR9 | HEAT repeat containing 9 | NM_001321395.2 | 92.9% | 92.8% | 8A>G;332_333ins120 |
| 5 | human | 256957 | HEATR9 | HEAT repeat containing 9 | XM_005278235.1 | 87% | 87% | 0_1ins102;230_231ins120 |
| 6 | human | 256957 | HEATR9 | HEAT repeat containing 9 | NR_135630.2 | 84.8% | (many diffs) | |
| 7 | human | 256957 | HEATR9 | HEAT repeat containing 9 | XM_017024424.2 | 82.6% | 80.8% | (many diffs) |
| 8 | human | 256957 | HEATR9 | HEAT repeat containing 9 | XR_934432.3 | 81.5% | (many diffs) | |
| 9 | human | 256957 | HEATR9 | HEAT repeat containing 9 | XR_934433.1 | 81.3% | (many diffs) | |
| 10 | human | 256957 | HEATR9 | HEAT repeat containing 9 | XR_934434.1 | 76.3% | (many diffs) | |
| 11 | human | 256957 | HEATR9 | HEAT repeat containing 9 | XR_254513.3 | 76% | (many diffs) | |
| 12 | human | 256957 | HEATR9 | HEAT repeat containing 9 | XM_006721802.3 | 68% | 68% | 0_1ins546 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1779
- ORF length:
- 1710
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcctgtgaa aaatcaactg atatctctga tgtctccagg tcaatgttcc 121 tgtacccatg gctggaatat ccagacaaga ccaaagaact cagaaaagcc atggctcctg 181 ttcatctgcc cttgtcctgc taccagatgc caaaggaaga gtttccccca agtccagagt 241 gctggaggca gcatccgagc aagccaaact cagtcccgta ctgctacttc aagaaacctg 301 agatctacac gcactggcac gacctgtatg atcagcgaga ggaaagggag gctgagaaga 361 tgttgaggaa aatgagagat gactgtaggt acatcaaaga ggtacatcaa acccacatca 421 aaatgttcca tctcccaatg agcaagctga ctataaaatc tgagatgcga tccaggccct 481 tagagcctac ccaggacccc ctgaagtggc aaagattaag ggaactcaca aaaagcctgg 541 aatctcccag agaggatgag cagttctatg cagcacaggc tctgggatgc ttacgcatca 601 gtgacaagtt tgtcatggag gcactacagc aggtggccca aactggtcca gagaaagtga 661 agtacgaggc ctaccgaacc ctggccatcc tgggttgcct gaataagcat gtgatccggg 721 ctctcatcaa acagctgaag gagaaaaatg agggtcaaag gatggagact ttgacggggc 781 tacgaatggc tcttaactcc tgggctgctg tctctaaaga caagaggact caagtcgggg 841 atgagggcaa gctggtgcct gtactacaga cactgatcaa gaagtcgtcc agtgaagcat 901 ctctggaggc agccctgtgc ctgggtttcc tgaggccttg cagcaacatg gtccaagagt 961 tcttgttgca gtgcctgtgc caaggactca agacccagcg gatgaaggca cttaggatgc 1021 tggtcaaggt gatgcacgtg cactcagccc cagtcatcaa ggccatccta gaccagctgt 1081 gttcttccag tgtccttgag gaccgctttg aagccaccca aatgctcaag accattgggc 1141 tggaacagat ccaggcacag gggctagagg aactcacatt taacctgctc aggaggaaga 1201 cgcataatga acccttcctt gctgtgaggc aggctgtggc tcaaactgtg gaagagctca 1261 agttgaagcc tacgatgatg aacttggtgg aggcacaact gatgaaccca gatgccactg 1321 cacgccagga agcagtcatc tctttgggtg tcctggggat ccgcagtcca caagtgttcc 1381 acttgcTCCT GGACTTACTA GATGCAGAAA ACCACCAGGC TGTGAAGAAG AGTCTACAAG 1441 AAACATTAAT CCTTTGTGCC TCAATTGATC CCTGGATCCA AAACAAGCTG AAAAACAAGG 1501 TTCTCTCTGT ATATGAGGCA CCTAAGACCA ATGTGAAGGC AGAGCCCACA AGGTTCCAGA 1561 AAGAGCCTGA GAACCCAGAA GAGTTAACTA TTCAAGACTT TCGACTTGCA AAGCTGAACC 1621 CCTTGTTTAT TGCAAAGTCC ATCACCAAAG TAGGCCAAAA GAAAACGCCT GCTTTCCCAC 1681 CGTGCTGCTC GAAACCACGA AAACATAGGC CACAGGTCAT AGGGCCCTGG CAGCCAAGGA 1741 TCAAGAAACA GCTCCGGGTC CTTGCTGAAA TTGCCAAATT GCCAACTTTC TTGTACAAAG 1801 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1861 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1921 ACGATATCTA GGGATAAAAT CTACCATTAC GCGTTAAGTC gacaatcaac ctctggatta 1981 caaaatttgt gaaagatt