Construct: ORF TRCN0000470696
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018321.1_s317c1
- Derived from:
- ccsbBroadEn_09104
- DNA Barcode:
- GCATTTATTTACTCTGACATATGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DYNLRB1 (83658)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470696
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 83658 | DYNLRB1 | dynein light chain roadbloc... | NM_014183.3 | 99.6% | 98.9% | 277A>C |
| 2 | human | 83658 | DYNLRB1 | dynein light chain roadbloc... | NM_001281729.1 | 78.5% | 63.1% | (many diffs) |
| 3 | human | 83658 | DYNLRB1 | dynein light chain roadbloc... | NM_001319157.1 | 77.1% | 65.6% | (many diffs) |
| 4 | human | 83658 | DYNLRB1 | dynein light chain roadbloc... | NM_001281727.1 | 65.2% | 64.5% | 0_1ins99;178A>C |
| 5 | human | 83658 | DYNLRB1 | dynein light chain roadbloc... | NM_001281728.1 | 65.2% | 64.5% | 0_1ins99;178A>C |
| 6 | human | 83658 | DYNLRB1 | dynein light chain roadbloc... | NM_177953.2 | 65.2% | 64.5% | 0_1ins99;178A>C |
| 7 | human | 83658 | DYNLRB1 | dynein light chain roadbloc... | NR_104032.1 | 35.9% | (many diffs) | |
| 8 | mouse | 67068 | Dynlrb1 | dynein light chain roadbloc... | NM_025947.3 | 90.9% | 95.8% | (many diffs) |
| 9 | mouse | 67068 | Dynlrb1 | dynein light chain roadbloc... | NM_001291108.1 | 83.6% | 87.5% | (many diffs) |
| 10 | mouse | 67068 | Dynlrb1 | dynein light chain roadbloc... | NR_111891.1 | 36.7% | (many diffs) | |
| 11 | mouse | 67068 | Dynlrb1 | dynein light chain roadbloc... | NR_111890.1 | 27.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 354
- ORF length:
- 288
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc agaggtggag gagacactga agcgactgca gagccagaag ggagtgcagg 121 gaatcatcgt cgtgaacaca gaaggcattc ccatcaagag caccatggac aaccccacCA 181 CCACCCAGTA TGCCAGCCTC ATGCACAGCT TCATCCTGAA GGCACGGAGC ACCGTGCGTG 241 ACATCGACCC CCAGAACGAT CTCACCTTCC TTCGAATTCG CTCCAAGAAA AATGAAATTA 301 TGGTTGCACC AGATAAAGAC TATTTCCTGA TTGTGATTCA GCATCCAACC GAATACCCAA 361 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 421 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 481 TATCTTGTGG AAAGGACGAG CATTTATTTA CTCTGACATA TGCACGCGTT AAGTCgacaa 541 tcaacctctg gattacaaaa tttgtgaaag att