Construct: ORF TRCN0000470774

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF008047.1_s317c1
Derived from:
ccsbBroadEn_00300
DNA Barcode:
TGGTAATAGCTAAACAGCCAAAAC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
RCC1 (1104)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000470774
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 1104 RCC1 regulator of chromosome con... NM_001048199.2 100% 100%
2 human 1104 RCC1 regulator of chromosome con... NM_001269.5 100% 100%
3 human 1104 RCC1 regulator of chromosome con... NM_001048195.3 96.1% 96.1% 73_123del
4 human 1104 RCC1 regulator of chromosome con... NM_001048194.3 93.1% 93.1% 73_165del
5 human 1104 RCC1 regulator of chromosome con... NR_030726.2 44.1% 1_299del;1563_2858del
6 human 1104 RCC1 regulator of chromosome con... NR_030725.2 44.1% 1_303del;1567_2862del
7 mouse 100088 Rcc1 regulator of chromosome con... NM_133878.3 89.2% 93.8% (many diffs)
8 mouse 100088 Rcc1 regulator of chromosome con... NM_001197082.1 86.5% 91% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1329
ORF length:
1263
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgtc acccaagcgc atagctaaaa gaaggtcccc cccagcagat gccatcccca
121aaagcaagaa ggtgaaggtc tcacacaggt cccacagcac agaacccggc ttggtgctga
181cactaggcca gggcgacgtg ggccagctgg ggctgggtga gaatgtgatg gagaggaaga
241agccggccct ggtatccatt ccggaggatg ttgtgcaggc tgaggctggg ggcatgcaca
301ccgtgtgtct aagcaaaagt ggccaggtct attccttcgg ctgcaatgat gagggtgccc
361tgggaaggga cacatcagtg gagggctcgg agatggtccc tgggaaagtg gagctgcaag
421agaaggtggt acaggtgtca gcaggagaca gtcacacagc agccctcacc gatgatggcc
481gtgtcttcct ctggggctcc ttccgggaca ataacggtgt gattggactg ttggagccca
541tgaagaagag catggtgcct gtgcaggtgc agctggatgt gcctgtggta aaggtggcct
601caggaaacga ccacttggtg atgctgacag ctgatggtga cctctacacc ttgggctgcg
661gggaacaggg ccagctaggc cgtgtgcctg agttatttgc caaccgtggt ggccggcaag
721gcctcgaacg actcctggtc cccaagtgtg tgatgctgaa atccagggga agccggggcc
781acgtgagatt ccaggatgcc ttttgtggtg cctatttcac ctttgccatc tcccatgagg
841gccacgtgta cggcttcggc ctctccaact accatcagct tGGAACTCCG GGCACAGAAT
901CTTGCTTCAT ACCCCAGAAC CTAACATCCT TCAAGAATTC CACCAAGTCC TGGGTGGGCT
961TCTCTGGTGG CCAGCACCAT ACAGTCTGCA TGGATTCGGA AGGAAAAGCA TACAGCCTGG
1021GCCGGGCTGA GTATGGGCGG CTGGGCCTTG GAGAGGGTGC TGAGGAGAAG AGCATACCCA
1081CCCTCATCTC CAGGCTGCCT GCTGTCTCCT CGGTGGCTTG TGGGGCCTCT GTGGGGTATG
1141CTGTGACCAA GGATGGTCGT GTTTTCGCCT GGGGCATGGG CACCAACTAC CAGCTGGGCA
1201CAGGGCAGGA TGAGGACGCC TGGAGCCCTG TGGAGATGAT GGGCAAACAG CTGGAGAACC
1261GTGTGGTCTT ATCTGTGTCC AGCGGGGGCC AGCATACAGT CTTATTAGTC AAGGACAAAG
1321AACAGAGCTG CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC
1381CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA
1441TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGATGGTAA TAGCTAAACA GCCAAAACAC
1501GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt

Download FASTA (ORF) (Full)