Construct: ORF TRCN0000470835
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004461.1_s317c1
- Derived from:
- ccsbBroadEn_06126
- DNA Barcode:
- CGCGCGATGTAGTTTTCTGCACTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TSC22D3 (1831)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470835
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1831 | TSC22D3 | TSC22 domain family member 3 | NM_001015881.2 | 99.5% | 100% | 231G>C |
| 2 | human | 1831 | TSC22D3 | TSC22 domain family member 3 | NM_004089.4 | 57.2% | 57.4% | 1_171del;402G>C |
| 3 | human | 1831 | TSC22D3 | TSC22 domain family member 3 | NM_001318468.1 | 38.3% | 38.5% | 1_369del;600G>C |
| 4 | human | 1831 | TSC22D3 | TSC22 domain family member 3 | NM_001318470.1 | 38.3% | 38.5% | 1_369del;600G>C |
| 5 | human | 1831 | TSC22D3 | TSC22 domain family member 3 | NM_198057.3 | 38.3% | 38.5% | 1_369del;600G>C |
| 6 | human | 1831 | TSC22D3 | TSC22 domain family member 3 | XM_005262099.1 | 38.3% | 38.5% | 1_369del;600G>C |
| 7 | human | 1831 | TSC22D3 | TSC22 domain family member 3 | XM_005262100.1 | 38.3% | 38.5% | 1_369del;600G>C |
| 8 | human | 1831 | TSC22D3 | TSC22 domain family member 3 | XM_005262102.1 | 38.3% | 38.5% | 1_369del;600G>C |
| 9 | human | 1831 | TSC22D3 | TSC22 domain family member 3 | XM_005262103.4 | 38.3% | 38.5% | 1_369del;600G>C |
| 10 | human | 1831 | TSC22D3 | TSC22 domain family member 3 | XM_011530884.1 | 38.3% | 38.5% | 1_369del;600G>C |
| 11 | human | 1831 | TSC22D3 | TSC22 domain family member 3 | XM_017029335.1 | 38.3% | 38.5% | 1_369del;600G>C |
| 12 | mouse | 14605 | Tsc22d3 | TSC22 domain family, member 3 | XM_006528503.3 | 83% | 88.7% | (many diffs) |
| 13 | mouse | 14605 | Tsc22d3 | TSC22 domain family, member 3 | NM_010286.4 | 48.6% | 51.8% | (many diffs) |
| 14 | mouse | 14605 | Tsc22d3 | TSC22 domain family, member 3 | XM_006528501.3 | 48.6% | 51.8% | (many diffs) |
| 15 | mouse | 14605 | Tsc22d3 | TSC22 domain family, member 3 | NM_001077364.1 | 33.2% | 35.3% | (many diffs) |
| 16 | mouse | 14605 | Tsc22d3 | TSC22 domain family, member 3 | XM_006528499.3 | 33.2% | 35.3% | (many diffs) |
| 17 | mouse | 14605 | Tsc22d3 | TSC22 domain family, member 3 | XM_006528500.3 | 33.2% | 35.3% | (many diffs) |
| 18 | mouse | 14605 | Tsc22d3 | TSC22 domain family, member 3 | XM_006528504.3 | 33.2% | 35.3% | (many diffs) |
| 19 | mouse | 14605 | Tsc22d3 | TSC22 domain family, member 3 | XM_006528505.3 | 33.2% | 35.3% | (many diffs) |
| 20 | mouse | 14605 | Tsc22d3 | TSC22 domain family, member 3 | NR_122039.1 | 11.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 182
- ORF end:
- 413
- ORF length:
- 231
- Sequence:
-
1 GTGGAGACTG AAGTTAGGCC AGCTTGGCAC TTGATGTAAT TCTCCTTGGA ATTTGCCCTT 61 TTTGAGTTTG GATCTTGGTT CATTCTCAAG CCTCAGACAG TGGTTCAAAG TTTTTTTCTT 121 CCATTTCAGG TGTCGTGAGG CTAGCATCGA TTGATCAACA AGTTTGTACA AAAAAGTGGG 181 CATGGATCTG GTGAAGAATC ATCTGATGTA TGCTGTGAGA GAGGAGGTGG AGATCCTGAA 241 GGAGCAGATC CGAGAGCTGG TGGAGAAGAA CTCCCAGCTA GAGCGTGAGA ACACCCTGTT 301 GAAGACCCTG GCAAGCCCAG AGCAGCTGGA GAAGTTCCAG TCCTGTCTGA GCCCTGAAGA 361 GCCAGCTCCC GAATCCCCAC AAGTGCCCGA GGCCCCTGGT GGTTCTGCGG TCTACCCAAC 421 TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA 481 TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT 541 ATCTTGTGGA AAGGACGACG CGCGATGTAG TTTTCTGCAC TAACGCGTTA AGTCgacaat 601 caacctctgg attacaaaat ttgtgaaaga tt