Construct: ORF TRCN0000470874
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015356.1_s317c1
- Derived from:
- ccsbBroadEn_02429
- DNA Barcode:
- TTACCTGGGGAATACAAAACAACC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RBM14 (10432)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470874
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10432 | RBM14 | RNA binding motif protein 14 | NM_006328.4 | 100% | 100% | |
| 2 | human | 10432 | RBM14 | RNA binding motif protein 14 | NM_001198836.2 | 22.9% | 22.7% | (many diffs) |
| 3 | human | 100526737 | RBM14-RBM4 | RBM14-RBM4 readthrough | NM_001198846.1 | 17.1% | 16.8% | (many diffs) |
| 4 | human | 10432 | RBM14 | RNA binding motif protein 14 | NM_001198837.2 | 17.1% | 16.7% | (many diffs) |
| 5 | mouse | 56275 | Rbm14 | RNA binding motif protein 14 | NM_019869.3 | 93.7% | 98.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2073
- ORF length:
- 2007
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa gatattcgtg ggcaacgtcg acggggcgga tacgactccg gaggagctgg 121 cagccctctt tgcgccctac ggcacggtca tgagctgcgc cgtcatgaaa cagttcgcct 181 tcgtgcacat gcgcgagaac gcgggcgcgc tgcgcgccat cgaagccctg cacggccacg 241 agctgcggcc ggggcgcgcg ctcgtggtgg agatgtcgcg cccaaggcct cttaatactt 301 ggaagatttt cgtgggcaat gtgtcggctg catgcacgag ccaggaactg cgcagcctct 361 tcgagcgccg cggacgcgtc atcgagtgtg acgtggtgaa agactacgcg tttgttcaca 421 tggagaagga agcagatgcc aaagccgcaa tcgcgcagct caacggcaaa gaagtgaagg 481 gcaagcgcat caacgtggaa ctctccacca agggtcagaa gaaggggcct ggcctggctg 541 tccagtctgg ggacaagacc aagaaaccag gggctgggga tacggccttc cctggaactg 601 gtggcttctc tgccaccttc gactaccagc aggcttttgg caacagcact ggtggctttg 661 atgggcaagc ccgtcagccc acaccaccct tctttggtcg cgaccgcagc cctctgcgcc 721 gttcacctcc ccgagcctct tatgtggctc ctctgacggc ccagccagct acctaccggg 781 cccagccgtc cgtgtcactg ggagctgcct acagggccca gccttctgcc tctttgggtg 841 ttggctatcg gactcagccc atgacagccc aggcagcctc ttaccgcgct cagccctctg 901 tctcccttgg ggcaccatac aggggccagc tggctagtcc tagctcccag tctgctgcag 961 cttcttcact cggcccatat ggtggagccc agccctcagc ctcggccctt tcctcctatg 1021 ggggtcaggc agctgcagct tcttcgctca actcctatgg ggctcagggt tcctcccttg 1081 cctcctatgg taaccagcca tcctcttacg gcgcccaggc tgcctcttcc tatggggttc 1141 gtgcagctgc ttcttcctac aacacccagg gagcagcttc ctccttaggc tcctacgggg 1201 ctcaggcagc ctcctatggg gcccagtctg cagcctcctc actagcttat ggagcccagg 1261 cagcttcata taatgcccag ccctcggcct cttacaatgc ccagtctgcc ccatatgctg 1321 cacagcaggc tgcttcctac tcttcccaac ctgctgccta tgtggcacag ccagccacag 1381 ctgctgccta tgccagccag ccagcagcct acgccgcaca agccactacc ccaatggctg 1441 gctcctatgg ggcccagccg gttgtgcaga cccagctgaa tagttacggg gcccaagcat 1501 caatgggcct ttcaggctcc tatggggctc agtcggctgc tgcggccact ggctcctatg 1561 gtgccgcagc agcctacggg gcccaacctt ctgccaccct ggcagctcct taccgcactc 1621 agtcatcagc ctcattggct gcttcctatg ctgcccagca gcatccccag gctgctgcct 1681 cctaccgcgg ccagccaggc aatgccTACG ATGGGGCAGG TCAGCCGTCT GCAGCCTACC 1741 TGTCCATGTC CCAGGGGGCC GTTGCCAACG CCAACAGCAC CCCGCCGCCC TATGAGCGTA 1801 CCCGCCTCTC CCCACCCCGG GCCAGCTACG ACGATCCCTA CAAAAAGGCT GTCGCCATGT 1861 CGAAAAGGTA TGGTTCCGAC CGGCGTTTAG CCGAGCTCTC TGATTACCGC CGTTTATCAG 1921 AGTCGCAGCT TTCGTTCCGC CGCTCGCCGA CAAAGTCCTC GCTGGATTAC CGTCGCCTGC 1981 CCGATGCCCA TTCCGATTAC GCACGCTATT CGGGCTCCTA TAATGATTAC CTGCGGGCGG 2041 CTCAGATGCA CTCTGGCTAC CAGCGCCGCA TGTACCCAAC TTTCTTGTAC AAAGTGGTTG 2101 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 2161 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATT 2221 ACCTGGGGAA TACAAAACAA CCACGCGTTA AGTCgacaat caacctctgg attacaaaat 2281 ttgtgaaaga tt