Construct: ORF TRCN0000470876
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012610.1_s317c1
- Derived from:
- ccsbBroadEn_00342
- DNA Barcode:
- GAAAACCACCGTTAAGGACGTCAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- COL8A1 (1295)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470876
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1295 | COL8A1 | collagen type VIII alpha 1 ... | NM_001850.5 | 100% | 100% | |
| 2 | human | 1295 | COL8A1 | collagen type VIII alpha 1 ... | NM_020351.4 | 100% | 100% | |
| 3 | mouse | 12837 | Col8a1 | collagen, type VIII, alpha 1 | NM_007739.2 | 87.9% | 93.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2298
- ORF length:
- 2232
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tgtgctgcct ggccctctgc agctgctggg agtgctgctt accatttccc 121 tgagttccat caggctcatt caggctggtg cctactatgg gatcaagccg ctgccacctc 181 aaattcctcc tcagatgcca ccacaaattc cacaatacca gcccctgggt cagcaagtac 241 ctcacatgcc tttggccaaa gatggccttg ccatgggcaa ggagatgccc cacttgcagt 301 atggcaaaga gtatccacac ctaccccaat atatgaagga aattcaaccg gcgccaagaa 361 tgggcaagga agccgtaccc aagaaaggca aagaaatacc attagccagt ttacgagggg 421 aacaaggtcc ccgtggagag cctggcccaa gaggaccacc tgggccccct ggtttgccag 481 gtcatgggat acctggaatt aaaggaaaac cagggccaca gggatatcca ggagttggaa 541 agccaggtat gcctggaatg ccagggaagc caggagccat gggcatgcct ggggcaaaag 601 gagaaattgg acagaaaggg gaaattgggc ctatggggat cccaggacca caaggacctc 661 cagggcctca tggacttcct ggcattggga agccaggtgg gccagggtta ccagggcaac 721 caggaccaaa gggtgatcga ggacccaaag gactaccagg acctcaaggc cttcggggtc 781 ctaaaggaga caagggcttc gggatgccag gtgcgccagg tgtaaagggg cctccaggga 841 tgcacggccc tcccggccct gttggactgc caggagtggg caaaccagga gtgacaggct 901 tccctgggcc ccagggcccc ctgggaaagc caggggctcc aggagaacct gggccacaag 961 gccctattgg ggtaccgggg gttcaaggac ctcctgggat acccggaatt ggaaagccag 1021 gccaggatgg gatcccaggc cagccaggat ttccaggtgg caaaggggag caaggactgc 1081 cagggctacc aggaccccca ggccttccag ggattgggaa accaggcttc ccaggaccca 1141 aaggtgaccg gggcatggga ggtgttcctg gggctcttgg accaagaggg gagaaaggac 1201 caataggtgc cccaggaata gggggtcctc caggagagcc aggcctgcct ggaatcccag 1261 gtcctatggg ccctccaggt gctattggtt ttcctggacc caaaggagaa ggtgggattg 1321 tagggccaca ggggccacca ggtcccaagg gtgagccagg gcttcaaggc ttcccaggaa 1381 agccaggttt ccttggtgaa gtagggcctc ctggcatgag gggtttgcca ggtcccatag 1441 ggcccaaggg ggaagctggg caaaaaggtg taccaggact ccctggtgtt ccagggcttc 1501 tcggacctaa gggagagcca ggaatcccag gggatcaggg tttacagggc cccccaggta 1561 tcccagggat tgggggccct agtggcccca ttggaccacc tgggattcca ggccccaaag 1621 gggagccggg cctcccaggg ccccctgggt tccctggtat agggaaaccc ggagtggcag 1681 gacttcatgg ccccccaggg aagcctggtg cccttggtcc tcaaggccag cctggccttc 1741 caggaccccc aggccctcca ggacctccag gacccccagc tgtgatgccc cctacaccac 1801 caccccaggg agagtatctg ccagatatgg ggctgggaat tgatggcgtg aaaccccccc 1861 atgcctacgg ggctaagaaa ggcaagaatg gagggccagc cTATGAGATG CCTGCATTTA 1921 CCGCCGAGCT AACCGCACCT TTCCCACCGG TGGGGGCCCC AGTGAAGTTT AACAAACTGC 1981 TGTATAACGG CAGACAGAAC TACAACCCGC AGACAGGCAT CTTCACCTGT GAGGTCCCTG 2041 GTGTCTACTA CTTTGCATAC CACGTTCACT GCAAGGGGGG CAACGTGTGG GTTGCTCTAT 2101 TCAAGAACAA CGAGCCCGTG ATGTACACGT ACGACGAGTA CAAAAAGGGC TTCCTGGACC 2161 AGGCATCTGG GAGTGCAGTG CTGCTGCTCA GGCCCGGAGA CCGGGTGTTC CTCCAGATGC 2221 CCTCAGAACA GGCTGCAGGA CTGTATGCCG GGCAGTATGT CCACTCCTCC TTTTCAGGAT 2281 ATTTATTGTA TCCCATGTAC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 2341 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 2401 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAGAAAACC ACCGTTAAGG 2461 ACGTCACACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt