Construct: ORF TRCN0000470911
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001463.1_s317c1
- Derived from:
- ccsbBroadEn_02671
- DNA Barcode:
- ATCCGACAGTATGCGCCCTAGTGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ECD (11319)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470911
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11319 | ECD | ecdysoneless cell cycle reg... | NM_007265.3 | 100% | 100% | |
| 2 | human | 11319 | ECD | ecdysoneless cell cycle reg... | NM_001135752.1 | 95.1% | 95.1% | 1126_1224del |
| 3 | human | 11319 | ECD | ecdysoneless cell cycle reg... | NM_001135753.1 | 93.3% | 93.3% | 782_783ins129 |
| 4 | human | 11319 | ECD | ecdysoneless cell cycle reg... | NR_024203.1 | 77.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1998
- ORF length:
- 1932
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga agaaaccatg aagcttgcta cgatggaaga cacagtggag tactgcctgt 121 tcctgatacc agatgagtca agggactcag ataaacataa agagattctt cagaagtaca 181 ttgagagaat aatcactcgg tttgcaccta tgctggtccc ctacatctgg cagaatcagc 241 ctttcaatct taaatataaa cctgggaaag gaggtgttcc tgctcatatg tttggcgtga 301 caaagtttgg ggataacatt gaggatgaat ggtttattgt ttatgtaata aagcagatca 361 caaaggaatt tccagagtta gtagcaagga ttgaagacaa tgatggtgaa ttcttgttaa 421 tagaagctgc tgactttctc cctaaatggc tggatcctga aaatagcacc aatagggtat 481 ttttctgcca tggggaattg tgtattatcc ctgcaccaag aaaatctgga gcagaatctt 541 ggttacccac cacaccccca acaattccac aagcattgaa tataatcaca gcacattcag 601 aaaaaatact tgcttcagaa tctatacgag ctgctgtgaa taggcgcatc agagggtacc 661 cagaaaaaat tcaggcctca cttcatcgag cacactgctt ccttccagct ggcattgtgg 721 cagtgctaaa gcagcgcccc agattggtgg ctgcagcagt ccaggcattt tacctacgag 781 accctattga cctgcgagct tgtcgtgttt tcaagacatt cttgcctgaa acacgaataa 841 tgacatcggt cacattcact aaatgtctat atgcacaatt ggtgcaacaa aggtttgtgc 901 cagaccggcg gagtggatac aggctgcctc ctccatctga tccccagtac cgagcccatg 961 aattgggcat gaaattggct catggatttg agatcttatg ctccaaatgt agcccacatt 1021 tttctgactg caagaaatcc cttgtgactg cctcaccact ctgggccagt ttccttgaaa 1081 gtctgaaaaa gaatgattac tttaagggac tgatagaagg ttctgctcag taccgggaaa 1141 ggctagaaat ggcagagaat tacttccagc tctcagtaga ctggccagaa agttctcttg 1201 ctatgagccc tggtgaagaa atcttaacct tattacagac aataccattt gatatagaag 1261 accttaagaa agaagcagct aatcttcccc cagaggatga tgaccagtgg ttagatctct 1321 caccagatca gctggaccag ctgctgcagg aagctgttgg caaaaaagaa tccgagtctg 1381 tttccaagga ggagaaggag cagaactatg acttaactga agtctcagag agcatgaaag 1441 ctttcatatc caaagtctca acccacaagg gagcagagct gcctcgagaa ccttctgagg 1501 ctccaatcac ttttgatgca gattcttttc ttaattattt tgataagatt ttagggccaa 1561 ggcctaatga gtcagattct gatgatctgg atgatgaaga ctttgaatgt ttagatagtg 1621 atgatgactt ggactttgaa acacacgaac ctggcgaaga ggcttcccTG AAAGGAACAC 1681 TTGATAATCT CAAGTCATAC ATGGCCCAGA TGGACCAGGA ACTAGCACAC ACCTGCATCA 1741 GCAAAAGTTT CACCACTAGG AACCAAGTGG AACCTGTATC CCAGACTACC GATAACAATT 1801 CAGATGAGGA AGATTCTGGT ACGGGAGAAT CTGTTATGGC ACCAGTAGAT GTAGACCTGA 1861 ACCTGGTTTC AAATATATTG GAATCCTATA GCTCCCAAGC TGGACTGGCA GGACCTGCTT 1921 CCAATCTTTT ACAAAGCATG GGAGTGCAGC TGCCTGACAA CACCGATCAC AGACCAACAA 1981 GTAAGCCAAC AAAAAATTAC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 2041 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 2101 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAATCCGAC AGTATGCGCC 2161 CTAGTGAACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt