Construct: ORF TRCN0000470985
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018949.2_s317c1
- Derived from:
- ccsbBroadEn_15334
- DNA Barcode:
- TCTAATACTCGTCGAGCTACCTAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LOC648152 (648152)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470985
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 545 | ATR | ATR serine/threonine kinase | NM_001354579.2 | 14.6% | 14.5% | (many diffs) |
| 2 | human | 545 | ATR | ATR serine/threonine kinase | XM_011512925.1 | 14.6% | 14.5% | (many diffs) |
| 3 | human | 545 | ATR | ATR serine/threonine kinase | NM_001184.4 | 14.2% | 14.2% | (many diffs) |
| 4 | human | 545 | ATR | ATR serine/threonine kinase | XM_011512924.1 | 14.2% | 14.2% | (many diffs) |
| 5 | human | 545 | ATR | ATR serine/threonine kinase | XR_001740179.2 | 13.6% | (many diffs) | |
| 6 | human | 545 | ATR | ATR serine/threonine kinase | XR_924148.2 | 13.6% | (many diffs) | |
| 7 | mouse | 245000 | Atr | ataxia telangiectasia and R... | XM_006511188.3 | 25.5% | 26.6% | (many diffs) |
| 8 | mouse | 245000 | Atr | ataxia telangiectasia and R... | XM_006511184.3 | 13% | 13.6% | (many diffs) |
| 9 | mouse | 245000 | Atr | ataxia telangiectasia and R... | NM_019864.1 | 13% | 13.6% | (many diffs) |
| 10 | mouse | 245000 | Atr | ataxia telangiectasia and R... | XR_001778905.1 | 11.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1206
- ORF length:
- 1137
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gatacctaca cttccatcaa ttctgggtac ccatgctaac catgctagcc 121 atgaaccatt tcctggacat tgggcctata ttgcagggtt tgatgatatg gtggaaattc 181 ttgcttctct tcagaaacca aagaagattt ctttaaaagg ctcagatgga aagttctaca 241 tcatgatgtg taagccaaaa gatgacctga gaaaggattg tagactaatg gaattcaatt 301 ccttgattaa taagtgctta agaaaagatg cagagtctcg tagaagagaa cttcatattc 361 gaacatatgc agttattcca ctaaatgatg aatgtgggat tattgaatgg gtgaacnaca 421 ctgctggttt gagacctatt ctgaccaaac tatataaaga aaagggagtg tatatgacag 481 gaaaannact tcgccagtgt atgctaccaa agtcagcagc tttatctgaa aaactcaaag 541 tattccgaga atttctcctg cccaggcatc ctcctatttt tcatgagtgg tttctgagaa 601 cattccctga tcntacatca tggtacagta gtagatcagc ttactgccgt tccactgcag 661 taatgtcaat ggttggttat attctggggc ttggagaccg tcatggtgaa aatattctct 721 ttgattcttt gactggtgaa tgcgtacatg tagatttcaa ttgtcttttc aataagggag 781 aaacctttga agttccagaa attgtgccat ttcgccTGAC TCATAATATG GTTAATGGAA 841 TGGGTCCTAT GGGAACAGAG GGTCTTTTTC GAAGAGCATG TGAAGTTACA ATGAGGCTGA 901 TGCGTGATCA GCGAGAGCCT TTAATGAGTG TCTTAAAGAC TTTTCTACAT GATCCTCTTG 961 TGGAATGGAG TAAACCAGTG AAAGGGCATT CCAAAGCGCC ACTGAATGAA ACTGGAGAAG 1021 TTGTCAATGA AAAGGCCAAG ACCCATGTTC TTGACATTGA GCAGCGACTA CAAGGTGTAA 1081 TCAAGACTCG AAATAGAGTG ACAGGACTGC CGTTATCTAT TGAAGGACAT GTGCATTACC 1141 TTATACAAGA AGCTACTGAT GAAAACTTAC TATGCCAGAT GTATCTTGGT TGGACTCCAT 1201 ATATGTTGCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1261 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1321 CTTGGCTTTA TATATCTTGT GGAAAGGACG ATCTAATACT CGTCGAGCTA CCTATACGCG 1381 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt