Construct: ORF TRCN0000470999
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012926.1_s317c1
- Derived from:
- ccsbBroadEn_02444
- DNA Barcode:
- GGGTACAAGGTCCCCCCTGGCCTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZNHIT1 (10467)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470999
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10467 | ZNHIT1 | zinc finger HIT-type contai... | NM_006349.3 | 100% | 100% | |
| 2 | human | 10467 | ZNHIT1 | zinc finger HIT-type contai... | XM_011515739.2 | 89.2% | 86.9% | (many diffs) |
| 3 | mouse | 70103 | Znhit1 | zinc finger, HIT domain con... | NM_001310747.1 | 88.3% | 97.4% | (many diffs) |
| 4 | mouse | 70103 | Znhit1 | zinc finger, HIT domain con... | NM_027318.4 | 85.8% | 94.1% | (many diffs) |
| 5 | mouse | 70103 | Znhit1 | zinc finger, HIT domain con... | NM_001310748.1 | 85.2% | 91.7% | (many diffs) |
| 6 | mouse | 70103 | Znhit1 | zinc finger, HIT domain con... | XM_006504497.3 | 85.2% | 91.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 528
- ORF length:
- 462
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt ggagaagaaa acttcggttc gcTCCCAGGA CCCCGGGCAG CGGCGGGTGC 121 TGGACCGGGC TGCCCGGCAG CGTCGCATCA ACCGGCAGCT GGAGGCCCTG GAGAATGACA 181 ACTTCCAGGA TGACCCCCAC GCGGGACTCC CTCAGCTCGG CAAGAGACTG CCTCAGTTTG 241 ATGACGATGC GGACACTGGA AAGAAAAAGA AGAAAACCCG AGGTGATCAT TTTAAACTTC 301 GCTTCCGAAA AAACTTTCAG GCCCTGTTGG AGGAGCAGAA CTTGAGTGTG GCCGAGGGCC 361 CTAACTACCT GACGGCCTGT GCGGGACCCC CATCGCGGCC CCAGCGCCCC TTCTGTGCTG 421 TCTGTGGCTT CCCATCCCCC TACACCTGTG TCAGCTGCGG TGCCCGGTAC TGCACTGTGC 481 GCTGTCTGGG GACCCACCAG GAGACCAGGT GTCTGAAGTG GACTGTGTGC CCAACTTTCT 541 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 601 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 661 GTGGAAAGGA CGAGGGTACA AGGTCCCCCC TGGCCTTACG CGTTAAGTCg acaatcaacc 721 tctggattac aaaatttgtg aaagatt