Construct: ORF TRCN0000471000
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008970.1_s317c1
- Derived from:
- ccsbBroadEn_05375
- DNA Barcode:
- AGTCGCACCAAACGCTTGGGTGCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ENDOV (284131)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471000
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 284131 | ENDOV | endonuclease V | NM_001164638.2 | 100% | 100% | |
| 2 | human | 284131 | ENDOV | endonuclease V | XM_005257246.3 | 93.7% | 91% | (many diffs) |
| 3 | human | 284131 | ENDOV | endonuclease V | NM_001164637.3 | 88.3% | 88.6% | 704_709delCACTTT;711_712ins87 |
| 4 | human | 284131 | ENDOV | endonuclease V | NM_001352760.2 | 85.4% | 85.4% | 227_361del |
| 5 | human | 284131 | ENDOV | endonuclease V | NM_001352761.2 | 80.9% | 78.2% | (many diffs) |
| 6 | human | 284131 | ENDOV | endonuclease V | XM_006721837.3 | 80.4% | 77.8% | (many diffs) |
| 7 | human | 284131 | ENDOV | endonuclease V | XM_011524663.1 | 78.1% | 75.5% | (many diffs) |
| 8 | human | 284131 | ENDOV | endonuclease V | XM_011524662.1 | 77.7% | 75.2% | (many diffs) |
| 9 | human | 284131 | ENDOV | endonuclease V | XM_017024511.1 | 77.5% | 75% | (many diffs) |
| 10 | human | 284131 | ENDOV | endonuclease V | XM_011524661.2 | 76.5% | 74% | (many diffs) |
| 11 | human | 284131 | ENDOV | endonuclease V | NM_173627.5 | 75.5% | 75.7% | 227_361del;839_844delCACTTT;846_847ins87 |
| 12 | human | 284131 | ENDOV | endonuclease V | XM_011524660.1 | 74.1% | 71.6% | (many diffs) |
| 13 | human | 284131 | ENDOV | endonuclease V | XM_011524669.2 | 72.9% | 73.1% | (many diffs) |
| 14 | human | 284131 | ENDOV | endonuclease V | XM_011524657.1 | 67.4% | 65.1% | (many diffs) |
| 15 | human | 284131 | ENDOV | endonuclease V | XM_011524671.2 | 66.3% | 64% | (many diffs) |
| 16 | human | 284131 | ENDOV | endonuclease V | XM_011524656.1 | 65.8% | 63.5% | (many diffs) |
| 17 | human | 284131 | ENDOV | endonuclease V | XM_011524655.1 | 65.5% | 63.2% | (many diffs) |
| 18 | human | 284131 | ENDOV | endonuclease V | XM_017024512.1 | 65.1% | 57.5% | (many diffs) |
| 19 | human | 284131 | ENDOV | endonuclease V | XM_011524670.1 | 64% | 61.8% | (many diffs) |
| 20 | human | 284131 | ENDOV | endonuclease V | XM_011524658.2 | 61.3% | 61.4% | (many diffs) |
| 21 | human | 284131 | ENDOV | endonuclease V | XM_017024510.1 | 61.1% | 60.9% | (many diffs) |
| 22 | human | 284131 | ENDOV | endonuclease V | XM_011524664.2 | 56.4% | 48% | (many diffs) |
| 23 | human | 284131 | ENDOV | endonuclease V | XM_011524666.1 | 56.4% | 48% | (many diffs) |
| 24 | human | 284131 | ENDOV | endonuclease V | XM_011524667.2 | 56.4% | 48% | (many diffs) |
| 25 | human | 284131 | ENDOV | endonuclease V | XM_017024508.1 | 56.4% | 48% | (many diffs) |
| 26 | human | 284131 | ENDOV | endonuclease V | XM_017024509.1 | 56.4% | 48% | (many diffs) |
| 27 | human | 284131 | ENDOV | endonuclease V | NR_148042.2 | 26.3% | (many diffs) | |
| 28 | human | 284131 | ENDOV | endonuclease V | NR_148043.2 | 23.9% | (many diffs) | |
| 29 | human | 284131 | ENDOV | endonuclease V | NR_148045.2 | 23.7% | (many diffs) | |
| 30 | human | 284131 | ENDOV | endonuclease V | NR_148041.2 | 22.9% | (many diffs) | |
| 31 | human | 284131 | ENDOV | endonuclease V | NR_148044.2 | 22.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 858
- ORF length:
- 792
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cctggaggcg gcgggagggc cgccggagga aacgctgtca ctgtggaaac 121 gggagcaagc tcggctgaag gcccacgtcg tagaccggga caccgaggcg tggcagcgag 181 accccgcctt ctcgggtctg cagagggtcg ggggcgttga cgtgtccttc gtgaaagggg 241 acagtgtccg cgcttgtgct tccctggtgg tgctcagctt ccctgagctc gaggtccttc 301 ttgtggatgg aaacggggta ctccaccacc gaggctttgg ggtggcctgc caccttggcg 361 tccttacaga cctgccgtgt gttggggtgg ccaagaaact tctgcaggtg gatgggctgg 421 agaacaacgc cctgcacaag gagaagatcc gactcctgca gactcgagga gactcattcc 481 ctctgctggg agactctggg actgtcctgg gaatggccct gaggagccac gaccgcagca 541 ccaggcccct ctacatcTCC GTGGGCCACA GGATGAGCCT GGAGGCCGCT GTGCGCCTGA 601 CTTGCTGCTG CTGCAGGTTC CGGATCCCAG AGCCCGTGCG CCAGGCTGAC ATCTGCTCCC 661 GAGAGCACAT CCGCAAGTCG CTGGGACTCC CCGGGCCACC CACACCGAGG AGCCCGAAGG 721 CGCAGAGGCC AGTGGCATGC CCCAAAGGAG ACTCCGGAGA GTCCTCAGGT GAGGGCCAGC 781 CCCCACAGGA CCACAGCCCA GGCCCCAGGA CAGCCCCAAG GCCAGGCTCC CAGGAGCAGG 841 CGGGCAAGGA CTGGCAGTAC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 901 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 961 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAAGTCGCA CCAAACGCTT 1021 GGGTGCGACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt