Construct: ORF TRCN0000471010
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015419.1_s317c1
- Derived from:
- ccsbBroadEn_01780
- DNA Barcode:
- GACCGGAGCCCCTCAGTGTGATTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- WNT7A (7476)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471010
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7476 | WNT7A | Wnt family member 7A | NM_004625.4 | 100% | 100% | |
| 2 | human | 7476 | WNT7A | Wnt family member 7A | XM_011534091.2 | 80.8% | 80.8% | 0_1ins201 |
| 3 | mouse | 22421 | Wnt7a | wingless-type MMTV integrat... | NM_009527.4 | 92.9% | 99.4% | (many diffs) |
| 4 | mouse | 22421 | Wnt7a | wingless-type MMTV integrat... | NM_001363757.1 | 39.7% | 38.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1113
- ORF length:
- 1047
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa ccggaaagcg cggcgctgcc tgggccacct ctttctcagc ctgggcatgg 121 tctacctccg gatcggtggc ttctcctcag tggtagctct gggcgcaagc atcatctgta 181 acaagatccc aggcctggct cccagacagc gggcgatctg ccagagccgg cccgacgcca 241 tcatcgtcat aggagaaggc tcacaaatgg gcctggacga gtgtcagttt cagttccgca 301 atggccgctg gaactgctct gcactgggag agcgcaccgt cttcgggaag gagctcaaag 361 tggggagccg ggaggctgcg ttcacctacg ccatcattgc cgccggcgtg gcccacgcca 421 tcacagctgc ctgtacccag ggcaacctga gcgactgtgg ctgcgacaaa gagaagcaag 481 gccagtacca ccgggacgag ggctggaagt ggggtggctg ctctgccgac atccgctacg 541 gcatcggctt cgccaaggtc tttgtggatg cccgggagat caagcagaat gcccggactc 601 tcatgaactt gcacaacaac gaggcaggcc gaaagatcct ggaggagaac atgaagctgg 661 aatgtaagtg ccacggcgtg tcaggctcgt gcaccaccaa gacgtgctgg accacactgc 721 cacagtttcg ggagctgggc tacgtgctca aggacaagta caacgaggcc gttcacgtgg 781 agcctgtgcg tgccagccgc aacaagcggc ccaccTTCCT GAAGATCAAG AAGCCACTGT 841 CGTACCGCAA GCCCATGGAC ACGGACCTGG TGTACATCGA GAAGTCGCCC AACTACTGCG 901 AGGAGGACCC GGTGACCGGC AGTGTGGGCA CCCAGGGCCG CGCCTGCAAC AAGACGGCTC 961 CCCAGGCCAG CGGCTGTGAC CTCATGTGCT GTGGGCGTGG CTACAACACC CACCAGTACG 1021 CCCGCGTGTG GCAGTGCAAC TGTAAGTTCC ACTGGTGCTG CTATGTCAAG TGCAACACGT 1081 GCAGCGAGCG CACGGAGATG TACACGTGCA AGTGCCCAAC TTTCTTGTAC AAAGTGGTTG 1141 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1201 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAGA 1261 CCGGAGCCCC TCAGTGTGAT TGACGCGTTA AGTCgacaat caacctctgg attacaaaat 1321 ttgtgaaaga tt