Construct: ORF TRCN0000471012

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF011657.1_s317c1
Derived from:
ccsbBroadEn_11333
DNA Barcode:
TAGTACGAGTCAGATGCACTGGCC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
USP6 (9098)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000471012
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 101060389 TBC1D3D TBC1 domain family member 3D XM_017024010.2 66.7% 59.7% (many diffs)
2 human 414060 TBC1D3C TBC1 domain family member 3C XM_006721908.2 50.1% 44.8% (many diffs)
3 human 101060376 TBC1D3L TBC1 domain family member 3L XM_006722247.2 50.1% 44.8% (many diffs)
4 human 101060389 TBC1D3D TBC1 domain family member 3D XM_006722253.2 50.1% 44.8% (many diffs)
5 human 101060351 TBC1D3K TBC1 domain family member 3K XM_011524174.1 50.1% 44.8% (many diffs)
6 human 102724862 TBC1D3I TBC1 domain family member 3I XM_011524185.2 50% 44.6% (many diffs)
7 human 729877 TBC1D3H TBC1 domain family member 3H XM_011525176.2 50% 44.6% (many diffs)
8 human 414060 TBC1D3C TBC1 domain family member 3C XM_011547459.2 35% 31.3% (many diffs)
9 human 101060376 TBC1D3L TBC1 domain family member 3L XM_006722244.2 35% 31.3% (many diffs)
10 human 101060389 TBC1D3D TBC1 domain family member 3D XM_006722250.2 35% 31.3% (many diffs)
11 human 101060351 TBC1D3K TBC1 domain family member 3K XM_011524173.1 35% 31.3% (many diffs)
12 human 414060 TBC1D3C TBC1 domain family member 3C XM_017024646.1 35% 31.3% (many diffs)
13 human 102724862 TBC1D3I TBC1 domain family member 3I XM_011524184.2 34.9% 31.1% (many diffs)
14 human 729877 TBC1D3H TBC1 domain family member 3H XM_011525175.2 34.8% 31.1% (many diffs)
15 human 414060 TBC1D3C TBC1 domain family member 3C XM_006721905.2 33.2% 29.7% (many diffs)
16 human 101060376 TBC1D3L TBC1 domain family member 3L XM_006722243.2 33.2% 29.7% (many diffs)
17 human 101060389 TBC1D3D TBC1 domain family member 3D XM_006722249.2 33.2% 29.7% (many diffs)
18 human 101060351 TBC1D3K TBC1 domain family member 3K XM_011524172.1 33.2% 29.7% (many diffs)
19 human 102724862 TBC1D3I TBC1 domain family member 3I XM_011524183.2 33.2% 29.5% (many diffs)
20 human 729877 TBC1D3H TBC1 domain family member 3H XM_011525174.2 33.1% 29.5% (many diffs)
21 human 414059 TBC1D3B TBC1 domain family member 3B XM_005257980.4 32.1% 28.6% (many diffs)
22 human 414060 TBC1D3C TBC1 domain family member 3C XM_005257981.3 32.1% 28.6% (many diffs)
23 human 101060376 TBC1D3L TBC1 domain family member 3L NM_001369499.1 32% 28.6% (many diffs)
24 human 101060321 TBC1D3G TBC1 domain family member 3G XM_005276914.3 32% 28.6% (many diffs)
25 human 414060 TBC1D3C TBC1 domain family member 3C XM_006721904.2 32% 28.6% (many diffs)
26 human 101060351 TBC1D3K TBC1 domain family member 3K XM_006722235.2 32% 28.6% (many diffs)
27 human 101060351 TBC1D3K TBC1 domain family member 3K XM_006722236.1 32% 28.6% (many diffs)
28 human 101060389 TBC1D3D TBC1 domain family member 3D XM_006722248.2 32% 28.6% (many diffs)
29 human 101060376 TBC1D3L TBC1 domain family member 3L XM_011524175.2 32% 28.6% (many diffs)
30 human 101060389 TBC1D3D TBC1 domain family member 3D XM_011524176.2 32% 28.6% (many diffs)
31 human 414060 TBC1D3C TBC1 domain family member 3C XM_011524814.2 32% 28.6% (many diffs)
32 human 102724862 TBC1D3I TBC1 domain family member 3I XM_006722223.3 32% 28.5% (many diffs)
33 human 729877 TBC1D3H TBC1 domain family member 3H XM_011525172.2 31.9% 28.5% (many diffs)
34 human 729877 TBC1D3H TBC1 domain family member 3H XM_011525173.2 31.9% 28.5% (many diffs)
35 human 100510707 TBC1D3J TBC1 domain family member 3J XM_024450524.1 21% 16.6% (many diffs)
36 human 9098 USP6 ubiquitin specific peptidase 6 XM_011524059.2 19.2% 19.1% 1_6delATGGAC;155_156ins183;496_2358del
37 human 100510707 TBC1D3J TBC1 domain family member 3J NM_001355571.1 15.3% 13.8% (many diffs)
38 human 9098 USP6 ubiquitin specific peptidase 6 XM_011524058.2 14.9% 14.8% 1_6delATGGAC;155_156ins183;496_3093del
39 human 9098 USP6 ubiquitin specific peptidase 6 XM_011524057.2 11.2% 11.2% 1_6delATGGAC;155_156ins183;496_4152del
40 human 9098 USP6 ubiquitin specific peptidase 6 NM_001304284.2 11.1% 11% 1_6delATGGAC;155_156ins183;496_4218del
41 human 9098 USP6 ubiquitin specific peptidase 6 NM_004505.4 11.1% 11% 1_6delATGGAC;155_156ins183;496_4218del
42 human 9098 USP6 ubiquitin specific peptidase 6 XM_011524050.1 11.1% 11% 1_6delATGGAC;155_156ins183;496_4218del
43 human 9098 USP6 ubiquitin specific peptidase 6 XM_011524051.2 11.1% 11% 1_6delATGGAC;155_156ins183;496_4218del
44 human 9098 USP6 ubiquitin specific peptidase 6 XM_011524052.2 11.1% 11% 1_6delATGGAC;155_156ins183;496_4218del
45 human 9098 USP6 ubiquitin specific peptidase 6 XM_011524053.2 11.1% 11% 1_6delATGGAC;155_156ins183;496_4218del
46 human 9098 USP6 ubiquitin specific peptidase 6 XM_011524054.2 11.1% 11% 1_6delATGGAC;155_156ins183;496_4218del
47 human 9098 USP6 ubiquitin specific peptidase 6 XM_011524055.2 11.1% 11% 1_6delATGGAC;155_156ins183;496_4218del
48 human 9098 USP6 ubiquitin specific peptidase 6 XM_011524056.2 11.1% 11% 1_6delATGGAC;155_156ins183;496_4218del
49 human 9098 USP6 ubiquitin specific peptidase 6 XM_017025290.1 11.1% 11% 1_6delATGGAC;155_156ins183;496_4218del
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
738
ORF length:
672
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggt agagaatgca gatagtttgc aggcacagga gcggaaggac atacttatga
121agtatgacaa gggacaccga gctgggctgc cagaggacaa ggggcctgag cccgttggaa
181tcaacagcag cattgatcgt tttggcattt tgcagtcctg cccctcctgg gagtcagagc
241cacaggaagg cccttgtcct cccttccctg tgccttctcc cgggctgagc cctgagctgg
301atagggacag agccagtcct ttctgggggt cggctcccag gcttgggcgg ctccaggccc
361tgtgcacgtc cTCAGCTCTG CCTGGGTTGC CTTACAGTGA GACGGAGCTG CCTCCTGTGA
421CTGCACGGGA GGCGAAGAAA ATTCGGCGGG AGATGACACG AACGAGCAAG TGGATGGAAA
481TGCTGGGAGA ATGGGAGACA TATAAGCACA GTAGCAAACT CATAGATCGA GTGTACAAGG
541GAATTCCCAT GAACATCCGG GGCCCGGTGT GGTCAGTCCT CCTGAACATT CAGGAAATCA
601AGTTGAAAAA CCCCGGAAGA TACCAGATCA TGAAGGAGAG GGGCAAGAGG TCATCTGAAC
661ACATCCACCA CATCGACCTG GACGTGAGGA CGACTCTCCG GAACCATGTC TTCTTTAGGG
721ATCGATATGG AGCCAAGTAC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA
781TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA
841GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGATAGTACG AGTCAGATGC
901ACTGGCCACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt

Download FASTA (ORF) (Full)