Transcript: Human XM_011525174.2

PREDICTED: Homo sapiens TBC1 domain family member 3H (TBC1D3H), transcript variant X3, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
TBC1D3H (729877)
Length:
3638
CDS:
1557..3323

Additional Resources:

NCBI RefSeq record:
XM_011525174.2
NBCI Gene record:
TBC1D3H (729877)

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011525174.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000267633 AGGGCTCATGGCTGGATAATT pLKO_005 3337 3UTR 100% 15.000 7.500 Y n/a
2 TRCN0000262160 GGGCTCATGGCTGGATAATTT pLKO_005 3338 3UTR 100% 15.000 7.500 Y TBC1D3F n/a
3 TRCN0000262158 ATAACAAGAATCGCCTTTAAG pLKO_005 2571 CDS 100% 13.200 6.600 Y TBC1D3F n/a
4 TRCN0000282133 CGGGACATAAGCGGGACATTA pLKO_005 2178 CDS 100% 13.200 6.600 Y TBC1D3F n/a
5 TRCN0000262161 GAGCGAGAGGACATCATTATG pLKO_005 1596 CDS 100% 13.200 6.600 Y TBC1D3F n/a
6 TRCN0000265682 GATAACAAGAATCGCCTTTAA pLKO_005 2570 CDS 100% 13.200 6.600 Y TBC1D3 n/a
7 TRCN0000282350 GGCTCATGGCTGGATAATTTC pLKO_005 3339 3UTR 100% 13.200 6.600 Y TBC1D3H n/a
8 TRCN0000443064 GGGACATAAGCGGGACATTAA pLKO_005 2179 CDS 100% 13.200 6.600 Y TBC1D3B n/a
9 TRCN0000262159 TTGGTTCCGCCATTATGATTT pLKO_005 3074 CDS 100% 13.200 6.600 Y TBC1D3F n/a
10 TRCN0000256846 ACAGGCGTTGATGCCGATAAC pLKO_005 2555 CDS 100% 10.800 5.400 Y n/a
11 TRCN0000421126 CACTGTGCTCAAGCATCTTAG pLKO_005 2684 CDS 100% 10.800 5.400 Y TBC1D3F n/a
12 TRCN0000118545 CAGGGATTTCACAGCCCAAAT pLKO.1 2403 CDS 100% 10.800 5.400 Y TBC1D3C n/a
13 TRCN0000118542 CGATAACAAGAATCGCCTTTA pLKO.1 2569 CDS 100% 10.800 5.400 Y TBC1D3C n/a
14 TRCN0000052405 CGGGACATTAAGGAAGCATAT pLKO.1 2189 CDS 100% 10.800 5.400 Y NPEPPSP1 n/a
15 TRCN0000414626 GATAATTTCCCTAGGCTTAAC pLKO_005 3351 3UTR 100% 10.800 5.400 Y TBC1D3F n/a
16 TRCN0000255682 GGATAATTTCCCTAGGCTTAA pLKO_005 3350 3UTR 100% 10.800 5.400 Y TBC1D3 n/a
17 TRCN0000262630 TGGTTCCGCCATTATGATTTC pLKO_005 3075 CDS 100% 10.800 5.400 Y TBC1D3H n/a
18 TRCN0000452941 TTGATGCCGATAACAAGAATC pLKO_005 2562 CDS 100% 10.800 5.400 Y TBC1D3B n/a
19 TRCN0000255680 TTGCAACCGGTTCGTTGATAC pLKO_005 2645 CDS 100% 10.800 5.400 Y TBC1D3 n/a
20 TRCN0000262628 AGGCGTTGATGCCGATAACAA pLKO_005 2557 CDS 100% 5.625 2.813 Y TBC1D3H n/a
21 TRCN0000118239 CGAGAGGACATCATTATGAAA pLKO.1 1599 CDS 100% 5.625 2.813 Y TBC1D3F n/a
22 TRCN0000118540 GCCTTGTTCCTCCTCTATCTT pLKO.1 2322 CDS 100% 5.625 2.813 Y TBC1D3B n/a
23 TRCN0000118541 CAAGCATCTTAGGGCCTCTAT pLKO.1 2693 CDS 100% 4.950 2.475 Y TBC1D3B n/a
24 TRCN0000118241 CCACTTGGAAAGTTCTCAGTT pLKO.1 3287 CDS 100% 4.950 2.475 Y TBC1D3F n/a
25 TRCN0000118546 GCTCATAGATCGAGCGTACAA pLKO.1 2018 CDS 100% 4.950 2.475 Y TBC1D3C n/a
26 TRCN0000118240 CCGATAACAAGAATCGCCTTT pLKO.1 2568 CDS 100% 4.050 2.025 Y TBC1D3F n/a
27 TRCN0000118237 CCAGATCACAAAGCCAACCAT pLKO.1 3479 3UTR 100% 3.000 1.500 Y TBC1D3F n/a
28 TRCN0000052404 GCGTTATTCCTAAAGTGGCTT pLKO.1 1020 5UTR 100% 2.640 1.320 Y NPEPPSP1 n/a
29 TRCN0000118238 GCCGATAACAAGAATCGCCTT pLKO.1 2567 CDS 100% 2.160 1.080 Y TBC1D3F n/a
30 TRCN0000118544 CCTGGCATATGAGGAGTATAA pLKO.1 2261 CDS 100% 0.000 0.000 Y TBC1D3C n/a
31 TRCN0000255681 GGACATTAAGGAAGCATATAT pLKO_005 2191 CDS 100% 15.000 7.500 Y TBC1D3 n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011525174.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_10145 pDONR223 100% 86.3% 86% None 159_341del;533C>T;944_945ins66 n/a
2 ccsbBroad304_10145 pLX_304 0% 86.3% 86% V5 159_341del;533C>T;944_945ins66 n/a
3 ccsbBroadEn_13681 pDONR223 100% 56% 44.5% None (many diffs) n/a
4 ccsbBroad304_13681 pLX_304 0% 56% 44.5% V5 (many diffs) n/a
5 TRCN0000467436 TGGATCCTATGCCTCATTGTTTAG pLX_317 29% 56% 44.5% V5 (many diffs) n/a
6 ccsbBroadEn_13692 pDONR223 100% 50.9% 40% None (many diffs) n/a
7 ccsbBroad304_13692 pLX_304 0% 50.9% 40% V5 (many diffs) n/a
8 TRCN0000471618 GCCCATAACCCTACGTGGCCAATT pLX_317 40.7% 50.9% 40% V5 (many diffs) n/a
9 ccsbBroadEn_12794 pDONR223 100% 49.3% 42.2% None (many diffs) n/a
10 ccsbBroad304_12794 pLX_304 0% 49.3% 42.2% V5 (many diffs) n/a
11 ccsbBroadEn_11333 pDONR223 100% 33.1% 29.5% None (many diffs) n/a
12 ccsbBroad304_11333 pLX_304 0% 33.1% 29.5% V5 (many diffs) n/a
13 TRCN0000471012 TAGTACGAGTCAGATGCACTGGCC pLX_317 54.4% 33.1% 29.5% V5 (many diffs) n/a
Download CSV

GPP Web Portal Terms of Service

Effective Date: December 8, 2025
By using this site, you agree to our terms and conditions below.

Overview of Terms

The data made available on this website were generated for research purposes and are not intended for clinical or commercial uses. Commercial use (or other use for profit-making purposes) of the GPP Web Portal and its tools, is not permitted under these terms and may require a separate license agreement from Broad or its contributors. For more information, please contact partnering@broadinstitute.org.

The original data may be subject to rights claimed by third parties, including but not limited to, patent, copyright, other intellectual property rights, biodiversity-related access and benefit-sharing rights. It is the responsibility of users of Broad Institute services to ensure that their use of the data does not infringe any of the rights of such third parties.

Any questions or comments concerning these Terms of Use can be addressed to: legal@broadinstitute.org.

By accessing and viewing this GPP Web Portal, you agree to the following terms and conditions:

Attribution

You agree to acknowledge the Broad Institute (e.g., in publications, services or products) for any of your use of its online services, databases or software in accordance with good scientific practice. You agree to use the acknowledgment wording provided for the relevant tools as indicated on the FAQ for each tool.

Updating the Terms of Use

We reserve the right to update these Terms of Use at any time. When alterations are inevitable, we will attempt to give reasonable notice of any changes by placing a notice on our website, but you may wish to check each time you use the website. The date of the most recent revision will appear on this, the "GPP Web Portal Terms of Use" page. If you do not agree to these changes, please do not continue to use our online services. We will also make available an archived copy of the previous Terms of Use for comparison.

Indemnification and Disclaimer of Warranties

You are using this GPP Web Portal at your own risk, and you hereby agree to hold Broad and its contributors and their trustees, directors, officers, employees, and affiliated investigators harmless for any third party claims which may arise from your use of the GPP Web Portal, the tools available therein, or any portion thereof. Further, you agree to indemnify Broad, its contributors, and its and their trustees, directors, officers, employees, affiliated investigators, students, and affiliates for any loss, costs, claims, damages, or other liabilities arising from any unpermitted commercial or profit-making use you make of the GPP Web Portal. The GPP Web Portal is a research tool and is provided "as is". Broad does not represent that the GPP Web Portal is free of errors or bugs or suitable for any particular tasks.

ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE, NONINFRINGEMENT, OR THE ABSENCE OF LATENT OR OTHER DEFECTS ARE DISCLAIMED. IN NO EVENT SHALL BROAD OR ITS CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THE GPP WEB PORTAL, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE.

Governing Law

The terms and conditions herein shall be construed, governed, interpreted, and applied in accordance with the internal laws of the Commonwealth of Massachusetts, U.S.A. Furthermore, by accessing, downloading, or using the Database, You consent to the personal jurisdiction of, and venue in, the state and federal courts within Massachusetts with respect to Your download or use of the Database.