Transcript: Human XM_011525174.2

PREDICTED: Homo sapiens TBC1 domain family member 3H (TBC1D3H), transcript variant X3, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
TBC1D3H (729877)
Length:
3638
CDS:
1557..3323

Additional Resources:

NCBI RefSeq record:
XM_011525174.2
NBCI Gene record:
TBC1D3H (729877)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011525174.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000267633 AGGGCTCATGGCTGGATAATT pLKO_005 3337 3UTR 100% 15.000 7.500 Y n/a
2 TRCN0000262160 GGGCTCATGGCTGGATAATTT pLKO_005 3338 3UTR 100% 15.000 7.500 Y TBC1D3F n/a
3 TRCN0000262158 ATAACAAGAATCGCCTTTAAG pLKO_005 2571 CDS 100% 13.200 6.600 Y TBC1D3F n/a
4 TRCN0000282133 CGGGACATAAGCGGGACATTA pLKO_005 2178 CDS 100% 13.200 6.600 Y TBC1D3F n/a
5 TRCN0000262161 GAGCGAGAGGACATCATTATG pLKO_005 1596 CDS 100% 13.200 6.600 Y TBC1D3F n/a
6 TRCN0000265682 GATAACAAGAATCGCCTTTAA pLKO_005 2570 CDS 100% 13.200 6.600 Y TBC1D3 n/a
7 TRCN0000282350 GGCTCATGGCTGGATAATTTC pLKO_005 3339 3UTR 100% 13.200 6.600 Y TBC1D3H n/a
8 TRCN0000443064 GGGACATAAGCGGGACATTAA pLKO_005 2179 CDS 100% 13.200 6.600 Y TBC1D3B n/a
9 TRCN0000262159 TTGGTTCCGCCATTATGATTT pLKO_005 3074 CDS 100% 13.200 6.600 Y TBC1D3F n/a
10 TRCN0000256846 ACAGGCGTTGATGCCGATAAC pLKO_005 2555 CDS 100% 10.800 5.400 Y n/a
11 TRCN0000421126 CACTGTGCTCAAGCATCTTAG pLKO_005 2684 CDS 100% 10.800 5.400 Y TBC1D3F n/a
12 TRCN0000118545 CAGGGATTTCACAGCCCAAAT pLKO.1 2403 CDS 100% 10.800 5.400 Y TBC1D3C n/a
13 TRCN0000118542 CGATAACAAGAATCGCCTTTA pLKO.1 2569 CDS 100% 10.800 5.400 Y TBC1D3C n/a
14 TRCN0000052405 CGGGACATTAAGGAAGCATAT pLKO.1 2189 CDS 100% 10.800 5.400 Y NPEPPSP1 n/a
15 TRCN0000414626 GATAATTTCCCTAGGCTTAAC pLKO_005 3351 3UTR 100% 10.800 5.400 Y TBC1D3F n/a
16 TRCN0000255682 GGATAATTTCCCTAGGCTTAA pLKO_005 3350 3UTR 100% 10.800 5.400 Y TBC1D3 n/a
17 TRCN0000262630 TGGTTCCGCCATTATGATTTC pLKO_005 3075 CDS 100% 10.800 5.400 Y TBC1D3H n/a
18 TRCN0000452941 TTGATGCCGATAACAAGAATC pLKO_005 2562 CDS 100% 10.800 5.400 Y TBC1D3B n/a
19 TRCN0000255680 TTGCAACCGGTTCGTTGATAC pLKO_005 2645 CDS 100% 10.800 5.400 Y TBC1D3 n/a
20 TRCN0000262628 AGGCGTTGATGCCGATAACAA pLKO_005 2557 CDS 100% 5.625 2.813 Y TBC1D3H n/a
21 TRCN0000118239 CGAGAGGACATCATTATGAAA pLKO.1 1599 CDS 100% 5.625 2.813 Y TBC1D3F n/a
22 TRCN0000118540 GCCTTGTTCCTCCTCTATCTT pLKO.1 2322 CDS 100% 5.625 2.813 Y TBC1D3B n/a
23 TRCN0000118541 CAAGCATCTTAGGGCCTCTAT pLKO.1 2693 CDS 100% 4.950 2.475 Y TBC1D3B n/a
24 TRCN0000118241 CCACTTGGAAAGTTCTCAGTT pLKO.1 3287 CDS 100% 4.950 2.475 Y TBC1D3F n/a
25 TRCN0000118546 GCTCATAGATCGAGCGTACAA pLKO.1 2018 CDS 100% 4.950 2.475 Y TBC1D3C n/a
26 TRCN0000118240 CCGATAACAAGAATCGCCTTT pLKO.1 2568 CDS 100% 4.050 2.025 Y TBC1D3F n/a
27 TRCN0000118237 CCAGATCACAAAGCCAACCAT pLKO.1 3479 3UTR 100% 3.000 1.500 Y TBC1D3F n/a
28 TRCN0000052404 GCGTTATTCCTAAAGTGGCTT pLKO.1 1020 5UTR 100% 2.640 1.320 Y NPEPPSP1 n/a
29 TRCN0000118238 GCCGATAACAAGAATCGCCTT pLKO.1 2567 CDS 100% 2.160 1.080 Y TBC1D3F n/a
30 TRCN0000118544 CCTGGCATATGAGGAGTATAA pLKO.1 2261 CDS 100% 0.000 0.000 Y TBC1D3C n/a
31 TRCN0000255681 GGACATTAAGGAAGCATATAT pLKO_005 2191 CDS 100% 15.000 7.500 Y TBC1D3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011525174.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_10145 pDONR223 100% 86.3% 86% None 159_341del;533C>T;944_945ins66 n/a
2 ccsbBroad304_10145 pLX_304 0% 86.3% 86% V5 159_341del;533C>T;944_945ins66 n/a
3 ccsbBroadEn_13681 pDONR223 100% 56% 44.5% None (many diffs) n/a
4 ccsbBroad304_13681 pLX_304 0% 56% 44.5% V5 (many diffs) n/a
5 TRCN0000467436 TGGATCCTATGCCTCATTGTTTAG pLX_317 29% 56% 44.5% V5 (many diffs) n/a
6 ccsbBroadEn_13692 pDONR223 100% 50.9% 40% None (many diffs) n/a
7 ccsbBroad304_13692 pLX_304 0% 50.9% 40% V5 (many diffs) n/a
8 TRCN0000471618 GCCCATAACCCTACGTGGCCAATT pLX_317 40.7% 50.9% 40% V5 (many diffs) n/a
9 ccsbBroadEn_12794 pDONR223 100% 49.3% 42.2% None (many diffs) n/a
10 ccsbBroad304_12794 pLX_304 0% 49.3% 42.2% V5 (many diffs) n/a
11 ccsbBroadEn_11333 pDONR223 100% 33.1% 29.5% None (many diffs) n/a
12 ccsbBroad304_11333 pLX_304 0% 33.1% 29.5% V5 (many diffs) n/a
13 TRCN0000471012 TAGTACGAGTCAGATGCACTGGCC pLX_317 54.4% 33.1% 29.5% V5 (many diffs) n/a
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