Construct: ORF TRCN0000471016
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008756.1_s317c1
- Derived from:
- ccsbBroadEn_03160
- DNA Barcode:
- TTACCCTGTGGCAGGCATAGTCTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- STMN3 (50861)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471016
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 50861 | STMN3 | stathmin 3 | NM_015894.4 | 100% | 100% | |
| 2 | human | 50861 | STMN3 | stathmin 3 | NM_001276310.1 | 93.8% | 93.8% | 0_1ins33 |
| 3 | human | 50861 | STMN3 | stathmin 3 | NR_075070.1 | 21.2% | 1_265del;556_652del;903_2538del | |
| 4 | mouse | 20262 | Stmn3 | stathmin-like 3 | NM_009133.3 | 89% | 96.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 606
- ORF length:
- 540
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cagcaccatt tccgcctaca aggagaagat gaaggagctg tcggtgctgt 121 cgctcatctg ctcctgcttc tacacacagc cgcaccccaa taccgtctac cagtacgggg 181 acatggaggt gaagcagctg gacaagcggg cctcaggcca gagcttcgag gtcatcctca 241 agTCCCCTTC TGACCTGTCC CCAGAGAGCC CTATGCTCTC CTCCCCACCC AAGAAGAAGG 301 ACACCTCCCT GGAGGAGCTG CAAAAGCGGC TGGAGGCAGC CGAGGAGCGG AGGAAGACGC 361 AGGAGGCGCA GGTGCTGAAG CAGCTGGCGG AGCGGCGCGA GCACGAGCGC GAGGTGCTGC 421 ACAAGGCGCT GGAGGAGAAT AACAACTTCA GCCGCCAGGC GGAGGAGAAG CTCAACTACA 481 AGATGGAGCT CAGCAAGGAG ATCCGCGAGG CACACCTGGC CGCACTGCGC GAGCGGCTGC 541 GCGAGAAGGA GCTGCACGCG GCCGAGGTGC GCAGGAACAA GGAGCAGCGA GAAGAGATGT 601 CGGGCTACCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 661 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 721 CTTGGCTTTA TATATCTTGT GGAAAGGACG ATTACCCTGT GGCAGGCATA GTCTGACGCG 781 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt