Construct: ORF TRCN0000471027

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF015276.1_s317c1
Derived from:
ccsbBroadEn_05210
DNA Barcode:
CTCATTGCAGACATACATACAACT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
C9orf72 (203228)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000471027
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 203228 C9orf72 C9orf72-SMCR8 complex subunit NM_001256054.2 100% 100%
2 human 203228 C9orf72 C9orf72-SMCR8 complex subunit NM_018325.5 100% 100%
3 human 203228 C9orf72 C9orf72-SMCR8 complex subunit NM_145005.6 46% 45.9% 666_666delGins778
4 mouse 73205 3110043O21Rik RIKEN cDNA 3110043O21 gene NM_001081343.1 90.4% 98.1% (many diffs)
5 mouse 73205 3110043O21Rik RIKEN cDNA 3110043O21 gene XM_006538292.3 90.4% 98.1% (many diffs)
6 mouse 73205 3110043O21Rik RIKEN cDNA 3110043O21 gene XM_006538293.3 90.4% 98.1% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1509
ORF length:
1443
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgtc gactctttgc ccaccgccat ctccagctgt tgccaagaca gagattgctt
121taagtggcaa atcaccttta ttagcagcta cttttgctta ctgggacaat attcttggtc
181ctagagtaag gcacatttgg gctccaaaga cagaacaggt acttctcagt gatggagaaa
241taacttttct tgccaaccac actctaaatg gagaaatcct tcgaaatgca gagagtggtg
301ctatagatgt aaagtttttt gtcttgtctg aaaagggagt gattattgtt tcattaatct
361ttgatggaaa ctggaatggg gatcgcagca catatggact atcaattata cttccacaga
421cagaacttag tttctacctc ccacttcata gagtgtgtgt tgatagatta acacatataa
481tccggaaagg aagaatatgg atgcataagg aaagacaaga aaatgtccag aagattatct
541tagaaggcac agagagaatg gaagatcagg gtcagagtat tattccaatg cttactggag
601aagtgattcc tgtaatggaa ctgctttcat ctatgaaatc acacagtgtt cctgaagaaa
661tagatatagc tgatacagta ctcaatgatg atgatattgg tgacagctgt catgaaggct
721ttcttctcaa tgccatcagc tcacacttgc aaacctgtgg ctgttccgtt gtagtaggta
781gcagtgcaga gaaagtaaat aagatagtca gaacattatg cctttttctg actccagcag
841agagaaaatg ctccaggtta tgtgaagcag aatcatcatt taaatatgag tcagggctct
901ttgtacaagg cctgctaaag gattcaactg gaagctttgt gctgcctttc cggcaagtca
961tgtatgctcc atatcccacc acacacatag atgtggatgt caatactgtg aagcagatgc
1021caccctgtca tgaacatatt tataatcagc gtagatacat gagatccgag ctgacagccT
1081TCTGGAGAGC CACTTCAGAA GAAGACATGG CTCAGGATAC GATCATCTAC ACTGACGAAA
1141GCTTTACTCC TGATTTGAAT ATTTTTCAAG ATGTCTTACA CAGAGACACT CTAGTGAAAG
1201CCTTCCTGGA TCAGGTCTTT CAGCTGAAAC CTGGCTTATC TCTCAGAAGT ACTTTCCTTG
1261CACAGTTTCT ACTTGTCCTT CACAGAAAAG CCTTGACACT AATAAAATAT ATAGAAGACG
1321ATACGCAGAA GGGAAAAAAG CCCTTTAAAT CTCTTCGGAA CCTGAAGATA GACCTTGATT
1381TAACAGCAGA GGGCGATCTT AACATAATAA TGGCTCTGGC TGAGAAAATT AAACCAGGCC
1441TACACTCTTT TATCTTTGGA AGACCTTTCT ACACTAGTGT GCAAGAACGA GATGTTCTAA
1501TGACTTTTTA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC
1561CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA
1621TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGACTCATT GCAGACATAC ATACAACTAC
1681GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt

Download FASTA (ORF) (Full)