Construct: ORF TRCN0000471027
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015276.1_s317c1
- Derived from:
- ccsbBroadEn_05210
- DNA Barcode:
- CTCATTGCAGACATACATACAACT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- C9orf72 (203228)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471027
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 203228 | C9orf72 | C9orf72-SMCR8 complex subunit | NM_001256054.2 | 100% | 100% | |
| 2 | human | 203228 | C9orf72 | C9orf72-SMCR8 complex subunit | NM_018325.5 | 100% | 100% | |
| 3 | human | 203228 | C9orf72 | C9orf72-SMCR8 complex subunit | NM_145005.6 | 46% | 45.9% | 666_666delGins778 |
| 4 | mouse | 73205 | 3110043O21Rik | RIKEN cDNA 3110043O21 gene | NM_001081343.1 | 90.4% | 98.1% | (many diffs) |
| 5 | mouse | 73205 | 3110043O21Rik | RIKEN cDNA 3110043O21 gene | XM_006538292.3 | 90.4% | 98.1% | (many diffs) |
| 6 | mouse | 73205 | 3110043O21Rik | RIKEN cDNA 3110043O21 gene | XM_006538293.3 | 90.4% | 98.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1509
- ORF length:
- 1443
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc gactctttgc ccaccgccat ctccagctgt tgccaagaca gagattgctt 121 taagtggcaa atcaccttta ttagcagcta cttttgctta ctgggacaat attcttggtc 181 ctagagtaag gcacatttgg gctccaaaga cagaacaggt acttctcagt gatggagaaa 241 taacttttct tgccaaccac actctaaatg gagaaatcct tcgaaatgca gagagtggtg 301 ctatagatgt aaagtttttt gtcttgtctg aaaagggagt gattattgtt tcattaatct 361 ttgatggaaa ctggaatggg gatcgcagca catatggact atcaattata cttccacaga 421 cagaacttag tttctacctc ccacttcata gagtgtgtgt tgatagatta acacatataa 481 tccggaaagg aagaatatgg atgcataagg aaagacaaga aaatgtccag aagattatct 541 tagaaggcac agagagaatg gaagatcagg gtcagagtat tattccaatg cttactggag 601 aagtgattcc tgtaatggaa ctgctttcat ctatgaaatc acacagtgtt cctgaagaaa 661 tagatatagc tgatacagta ctcaatgatg atgatattgg tgacagctgt catgaaggct 721 ttcttctcaa tgccatcagc tcacacttgc aaacctgtgg ctgttccgtt gtagtaggta 781 gcagtgcaga gaaagtaaat aagatagtca gaacattatg cctttttctg actccagcag 841 agagaaaatg ctccaggtta tgtgaagcag aatcatcatt taaatatgag tcagggctct 901 ttgtacaagg cctgctaaag gattcaactg gaagctttgt gctgcctttc cggcaagtca 961 tgtatgctcc atatcccacc acacacatag atgtggatgt caatactgtg aagcagatgc 1021 caccctgtca tgaacatatt tataatcagc gtagatacat gagatccgag ctgacagccT 1081 TCTGGAGAGC CACTTCAGAA GAAGACATGG CTCAGGATAC GATCATCTAC ACTGACGAAA 1141 GCTTTACTCC TGATTTGAAT ATTTTTCAAG ATGTCTTACA CAGAGACACT CTAGTGAAAG 1201 CCTTCCTGGA TCAGGTCTTT CAGCTGAAAC CTGGCTTATC TCTCAGAAGT ACTTTCCTTG 1261 CACAGTTTCT ACTTGTCCTT CACAGAAAAG CCTTGACACT AATAAAATAT ATAGAAGACG 1321 ATACGCAGAA GGGAAAAAAG CCCTTTAAAT CTCTTCGGAA CCTGAAGATA GACCTTGATT 1381 TAACAGCAGA GGGCGATCTT AACATAATAA TGGCTCTGGC TGAGAAAATT AAACCAGGCC 1441 TACACTCTTT TATCTTTGGA AGACCTTTCT ACACTAGTGT GCAAGAACGA GATGTTCTAA 1501 TGACTTTTTA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1561 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1621 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGACTCATT GCAGACATAC ATACAACTAC 1681 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt