Construct: ORF TRCN0000471028
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011957.1_s317c1
- Derived from:
- ccsbBroadEn_15987
- DNA Barcode:
- CCGGGAAATGGTCAAATTATAAAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- VEPH1 (79674)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471028
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79674 | VEPH1 | ventricular zone expressed ... | NM_001167915.2 | 100% | 100% | |
| 2 | human | 79674 | VEPH1 | ventricular zone expressed ... | NM_001167916.2 | 100% | 100% | |
| 3 | human | 79674 | VEPH1 | ventricular zone expressed ... | NM_001167917.1 | 82.9% | 82.6% | 530T>G;531_532ins108 |
| 4 | human | 79674 | VEPH1 | ventricular zone expressed ... | XM_024453749.1 | 82.9% | 82.6% | 530T>G;531_532ins108 |
| 5 | human | 79674 | VEPH1 | ventricular zone expressed ... | XM_024453750.1 | 82.9% | 82.6% | 530T>G;531_532ins108 |
| 6 | human | 79674 | VEPH1 | ventricular zone expressed ... | NM_001167911.2 | 24.9% | 22.4% | (many diffs) |
| 7 | human | 79674 | VEPH1 | ventricular zone expressed ... | NM_001167912.2 | 23.6% | 21.2% | (many diffs) |
| 8 | human | 79674 | VEPH1 | ventricular zone expressed ... | NM_024621.2 | 23.6% | 21.2% | (many diffs) |
| 9 | human | 79674 | VEPH1 | ventricular zone expressed ... | XM_024453746.1 | 23.6% | 21.2% | (many diffs) |
| 10 | human | 79674 | VEPH1 | ventricular zone expressed ... | XM_024453747.1 | 23.6% | 21.2% | (many diffs) |
| 11 | human | 79674 | VEPH1 | ventricular zone expressed ... | XM_024453748.1 | 23.6% | 21.2% | (many diffs) |
| 12 | human | 79674 | VEPH1 | ventricular zone expressed ... | XM_011513134.2 | 20.6% | 16% | (many diffs) |
| 13 | human | 79674 | VEPH1 | ventricular zone expressed ... | XM_011513135.2 | 18.2% | 15.8% | (many diffs) |
| 14 | mouse | 72789 | Veph1 | ventricular zone expressed ... | XM_011240244.2 | 67.8% | 64.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 705
- ORF length:
- 639
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca tcaactgttc agactggttt tgggacaaaa agatctttca cgagctgggg 121 acctcttctc cttagatgac tctgagattg aagacagcct tacagaagct ttggagcaaa 181 ttaagataat tagctcatct tcagattacc aaaccaataa caatgaccag gcagtagttg 241 aaatctgtat cacaagaatc acaacagcca TCAGAGAGAC CGAGTCCATT GAAAAGCATG 301 CAAAGGCCCT TGTGGGGCTC TGGGACTCCT GCTTGGAACA TAACCTGAGA CCCTTTGGGA 361 AAGACGAAGA CACTCCTCAT GCAAAAATCG CATCTGATAT CATGAGTTGC ATTTTACAGA 421 ATTACAACCG ACCCCCAGTG ATGGCATTAG CCATCCCCAT TGCAGTGAAA TTCCTCCACA 481 GAGGCAACAA GGAACTGTGC AGGAATATGT CTAACTACCT GTCTCTGGCT GCAATTACCA 541 AGGCAGATCT CCTGGCTGAT CACACGGAAG TTATAGTAAA GAGCATACTC CAAGGTATGG 601 TGCGAAAGTT GAGCTTGGGG ACATGTTTTG GGAGATATTT AAAAGTATTT TCATCAAGCA 661 TTTATGGTTT GTGGGAAGCA AGACCTAGAG TATTAGAAGC AAATTACCCA ACTTTCTTGT 721 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 781 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 841 GAAAGGACGA CCGGGAAATG GTCAAATTAT AAATACGCGT TAAGTCgaca atcaacctct 901 ggattacaaa atttgtgaaa gatt